African Journal of
Biotechnology

  • Abbreviation: Afr. J. Biotechnol.
  • Language: English
  • ISSN: 1684-5315
  • DOI: 10.5897/AJB
  • Start Year: 2002
  • Published Articles: 12482

Full Length Research Paper

Preliminary characterization of Rhizobium strains isolated from chickpea nodules

ÇiÄŸdem Küçük1* and Merih Kıvanç2
1Harran University, Faculty of Arts and Science, Department of Biology, Åžanlıurfa, Turkey. 2Anadolu University, Faculty of Science, Department of Biology, 26470 Eskisehir, Turkey.
Email: [email protected]

  •  Published: 18 March 2008

Abstract

A total of 28 Rhizobium strains from chickpea nodules were characterized on the basis of morphological, cultural and biochemical characteristics. Most strains produced abundant extracellular polysaccharides, were tolerant to 0.5 M NaCI (53%) and a temperature of 40oC (75%). The majority of the strains showed an intrinsic resistance to the antibiotics (µg ml-1) streptomycin (100), kanamycin (50), erytromycin (30), chloramphenicol (200) and penicilin (25). In vitro antibiosis assays indicated that Rhizobium strains from chickpea nodules exercised an antagonism against Ascocyhta rabiei the agent of ascocyhta blight disease of chickpea.

 

Key words: Rhizobium, chickpea (Cicer arietinum L.), preliminary characterization.

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This article is published under the terms of the Creative Commons Attribution License 4.0

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