African Journal of
Biotechnology

  • Abbreviation: Afr. J. Biotechnol.
  • Language: English
  • ISSN: 1684-5315
  • DOI: 10.5897/AJB
  • Start Year: 2002

AJB Articles in press

Article in Press

Application of molecular techniques in plant disease management: A review

Plant diseases are a major challenge in crop production. They are caused by nematodes, bacteria, fungi, viruses as well as plant nutritional factors. Diseases interfere with the normal physiological and metabolic processes of plants. This results in various effects including wilting, stunting, yellowing and death of plant tissues and organs. Crop losses due to diseases occur in form of reduced yield, poor quality produce,...

Author(s):Olivier Dayou, Maina Mwangi , Andrew Ogolla Egesa, Peter Munyao Muteti and Careen Ihazano Chumba

Article in Press

Nuclear isolation and purification using SDS/urea (NIPSU) method for efficient and rapid extraction of high-purity genomic DNAs from Jatropha curcas L: A comparative analysis of DNA isolation protocols

Jatropha curcas L. (Jatropha) has gained popularity for its potential use in biodiesel production in arid regions, and its yield improvement by molecular breeding has been anticipated. However, Jatropha is known for its recalcitrance in the extraction of pure genomic DNAs. In this study, four DNA extraction methods were comparatively examined for their efficiency and quality of genomic DNA isolations from Jatropha....

Author(s):Yoshihiko Nanasato, Ryo Uenoyama, Kaede Tomooka, Atsushi Kato, and Kinya Akashi,

Article in Press

Evaluation of Curcuma zerumbet (Zingibaraceae) rhizome extracts sub-acute toxicity on Wistar rats

Plant extracts have been lately used by the population to treat various types of diseases, and this has been notably encouraged by the World Health Organization (WHO). Curcuma zerumbet (Zingiberaceae) belonging to the family of the Zingiberaceae, is herbaceous, perennial and, utilized by the population to treat gastric disorders. However, data on the subacute toxicity of this species are scarce in the literature....

Author(s):Federal University of Amazonas/Post-graduation Program in Biotechnology, General Rodrigo Octávio Avenue, , Coroado , CEP -, Manaus-AM, Brazil.

Article in Press

Biotransformation of the residual liquid from the wet coffee benefit by Kluyveromyces marxianus

The search for biotechnological alternatives for the use of residuals generated in the agro-industrial processing of coffee is a current problem. This study evaluated the biotransformation of the liquid residual of the humid coffee benefit using the yeast Kluyveromyces marxianus CCEBI 2011. It was demonstrated that this strain is able to effectively use the reducing and neutral sugars in 24 h, for a yield of 40% in the...

Author(s):Kluyveromyces marxianus, pectinolitic yeast, coffee residuals.

Article in Press

Performance of three epigeic earthworm species, Eisenia fetida, Eisenia andrie and Dendrobanae veneta, in managing Rose and Carnation wastes in flower industries

The horticulture sector in Ethiopia is being challenged by multifaceted hitches; among which managing excess wastes produced from the farms and minimizing the cost of inorganic fertilizers are the major ones. The performance of three epigeic earthworm species, Eisenia fetida, Eisenia andrie and Dendrobanae veneta, in managing flower (Rose and Carnation) wastes through vermicomposting was evaluated. The study was done using...

Author(s):SAID MOHAMMED HASSEN

Article in Press

IN VITRO REGENERATION OF NEOCARYA MACROPHYLLA (SABINE) PRANCE, WILD FRUIT OF NIGER.

Neocarya macrophylla is a spontaneous fruit species that constitutes the dominant population of Dallol Bosso in southwestern Niger. In order to test the in vitro regeneration ability of this species, micro-propagation was applied to micro-cuttings from young almond seedlings. The MS basal medium was enriched with several concentrations of NAA (0; 0.5 and 1 mg.L-1) and BAP (0; 0.5; 1; 1.5; 2; 3 mg.L-1). The first results...

Author(s): ABOUBACAR Kolafane

Article in Press

Clausenidin upregulated p53 and caused apoptosis in HT-29 tumor cell lines

Introduction: Clausenidin is a pyranocoumarin majorly found in medicinal herbs of the Rutaceae family used to treat cancer patients locally in Asia. The compound is presumed to have anti-cancer cell effect but its exact mechanism of action is still unknown. Aim: The study aimed to evaluate the effect of pure clausenidin on p53-mediated apoptosis as well as other cell death pathways in colon cancer (HT-29) cell lines....

Author(s):Peter M Waziriab, Rasedee Abdullahc, Rosita Roslia, Abdul Rahman Omard, Ahmad Bustamam Abdula, Nur Kartinee Kassime, Ibrahim Malamia, Imaobong C Ettia, Palanisamy Arulselvand and Ashwaq Shakir Al-Abboodia.

Article in Press

Comparative analysis of the chemical compositions of indigenous watermelon (Citrullus lanatus) seeds from two districts in Limpopo Province, South Africa

Seeds from different indigenous watermelons were analysed for their chemical composition such as phenolics, proteins, oils, minerals, ash and fibre content as well as antioxidant activity measured in methanol extract using 2.2-diphenyl-1-picrylhydrazyl (DPPH) assay. The morphological variations of the watermelons, which revealed diversity in terms of fruit and seed colour was also assessed. Average percentages for lipid...

Author(s):PHETOLE MANGENA,Phatlane William Mokwala,Emmanuel Alpheus Mogotlane

Article in Press

Evaluation of plant aqueous extracts as anti-diabetic agents on alloxan induced rabbits

In this study, effect of Barley (Hordeum vulgare), Black plum (Syzygium cumini) and Chinese tree leaves (Pistacia chinesis) aqueous extracts was investigated on serum glucose, cholesterol, urea levels and body weight in normal and alloxan-induced diabetic male rabbits. Rabbits were kept in fasting condition for 12 h with 150 mg/Kg dose of alloxan monohydrate was introduced. From 7th day of diabetes induction, blood serum...

Author(s):Hadia Akber Samoo ✝, Jagarwanti Maheshwari✝, Atta Muhammad Babar, Ather Hameed Sheikh ,Imrana Khushk, Muhammad Aqeel Bhutto, Jawaid Ahmad Khokhar, Abdul Sattar Qureshi

Article in Press

Protocol optimization of in vitro propagation of an endangered endemic Ethiopia medicinal plant, Echinops kebericho (Mesfin), using shoot tip

Echinops kebericho (Mesfin)isthe most important and critically endangered endemic Ethiopian medicinal plant. This plant is threatened due to over harvesting of its roots for medicinal purpose, and from seed viability and seed germination problem. The aim of this research was to build up an efficient in vitro propagation protocol for E. kebericho using shoot tip explants. Shoots excised from in vitro germinated seedlings...

Author(s):Muluken Enyew; Tileye Feyissa

Article in Press

Rapid and efficient plant regeneration from shoot apical meristems of finger millet [Eleusinecoracana (L.) Gaertn.] via direct organogenesis

A simple and efficient plant regeneration system via direct organogenesis was established in finger millet using in vitro derived shoot apical meristems. Six varieties; GBK-043128, GBK-043094, GBK-043050, GBK-043137, GBK-043122 and GBK-043124 were evaluated. MS medium was used for cotyledonary germination. Maximum number of shoots (84.33%) was observed in variety GBK-043128 while GBK-043094 had the least germination...

Author(s):Asunta Mukami, Alex Ngetich, Cecilia Mweu, Mutemi Muthangya, Richard O. Oduor Mathew Ngugi, and Wilton Mbinda

Article in Press

Development of phage mediated quartz crystal microbalance (QCM) sensor for the detection of Escherichia coli, a food-borne pathogen

This study presents the development of phage mediated quartz crystal microbalance (QCM) sensor for the detection of Escherichia coli. Bacteriophage is very specific to host bacterial cell also known as parasite for bacteria. The Electrochemical Quartz Crystal Microbalance (EQCM) is a very sensitive device that measures a mass change per unit area by measuring the change in resonant frequency of a quartz crystal. A...

Author(s):Vipin Singh and Pranay Jain

Article in Press

Morphological diversity studies among sugarcane (Saccharum spp.) somaclones generated through callus culture

Callogenesis is one of the tools in sugarcane tissue culture for generating agronomically significant variation. Tissue culture derived variations are known as somaclonal variation. The study was carried out to investigate the effect of MS media supplemented with 1.0 mgL-1 of NAA (naphthalene acetic acid) and 2.0, 2.5, 3.0, 3.5 and 4.0 mgL-1 of 2,4-D (2,4-dichlorophenoxy acetic acid) on somaclonal variation in in vitro...

Author(s):Richard Wekesa, Justus M. Onguso, Bernard A. Nyende and Leonard S. Wamocho

Article in Press

Biodegradation of crude oil by Bacillus species isolated from different sources

The consequences of oil pollution to the environment and human health as a result of exploration activities, transportation and usage of petroleum compounds, has become a serious challenge to environmental scientists. This study evaluates the potential of using Bacillus subtilis in biodegradation of petroleum compounds. Ten Bacillus strains from non-petroleum contaminated sites were identified on the basis of microscopy...

Author(s):Mohammed Inuwa Ja’afaru, Chimezie Godwin Azuchukwuene and Aisha Sa’id Shittu

Article in Press

Analytical quantification of copper in frogs (Rana tigrina) found from various aquatic habitats

This study focused on the quantitative determination of Cu metal in various physical parts of frogs by using the Atomic Absorption Spectrometry technique). Cu was measured (2.72±0.77 µg/g) in kidney of Rana tigrina captured from industrial site and in R. tigrina captured from non-industrial site (28.10±27.02 µg/g). Similarly, 27.56±9.92 µg/g Cu was discovered in liver of R. tigrina captured from industrial site and...

Author(s):Muhammad Nasir, Tariq Mahmood Ansari, Ghulam Yasin, Aleem Ahmad Khan and Muhammad Shoaib

Article in Press

Marker-assisted pyramiding of Xa21 and Xa7 genes conferring resistance to bacterial leaf blight in indica cultivar Bacthom7

Bacterial leaf blight (BLB) of rice is one of the most destructive diseases affecting rice fields. Xanthomonas oryzae pv. oryzae (Xoo) is the causal agent of BLB. Two BLB resistance genes, Xa21 and Xa7, were transferred into the susceptible indica cultivar Bacthom7 (BT7) by using marker-assisted selection with markers pTA248 for Xa21 and ID7 for Xa7. Improved BT7 lines carrying the two resistance genes were inoculated with...

Author(s):Nguyen Thi Thu#, Vu Hong Quang#, Mai Van Tan#, Vu Đuc Lam, Nakano Toshitsugu, Nguyen Thi Hue, Nong Thi Hue, Nguyen Quoc Trung, Nguyen Viet Long, Nguyen Van Hoan and Vu Van Liet

Article in Press

Isolation and identification of bacterial endophytes from Crinum macowanii Baker

The widespread distribution of Crinum macowanii across the African continent has entrenched the plant’s medicinal usage in treating diverse diseases. While its phytochemistry is well established, its microbial symbionts and their utility have not been described. As such, five bacterial endophytes, viz. Staphylococcus species C2, Staphylococcus species C3, Bacillus species C4, Acinetobacter species C5 and Staphylococcus...

Author(s):Rebotiloe F. Morare, Eunice Ubomba-Jaswa, and Mahloro H. Serepa-Dlamini

Article in Press

Psychrotrophic proteolytic bacteria and their characterization from landfill soil under temperate climatic conditions of Kashmir Himalaya

During cold season in Kashmir valley, the decomposition of municipal solid waste slows down due to slowing down of microbial activity as a result, the waste gets piled up and its management becomes a greater challenge. Psychrotrophic proteolytic bacteria play an important role in the degradation of protenaceous materials of the waste stream under low temperatures that produces nitrogen sources for rapid functioning of...

Author(s):Basharat Hamid, Arshid Jehangir and Zahoor Ahmad Baba

Article in Press

Antioxidant activity of essential oil of three cultivars of Amomum subulatum and standardization of high performance thin layer chromatography (HPTLC) method for the estimation of 1,8-cineole

Essential oils of the fruits of three cultivars of Amomum subulatum (Family-Zingiberaceae), such as varlangy, seremna and sawney were isolated. Antioxidant potential was determined using 1,1-diphenyl-2-picrylhydrazyl (DPPH) and 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulphonic acid) (ABTS) scavenging and FeCl3-reducing models and the presence of 1,8-Cineole was quantified using the developed high performance thin layer...

Author(s):Aftab Alam and Rita Singh Majumdar

Article in Press

Holistic plant regeneration protocol from callus cultures of Salvia moorcroftiana, a medicinal herb

Salvia moorcroftiana is a medicinal herb of the family Lamiaceae. The objective of this study was to develop an in vitro protocol for the rapid propagation of this species. Organogenesis by means of callus culture was carried out on Murashige and Skoog medium by the transfer of callus from an induction medium supplemented with α-naphtalenacetic acid (NAA, 1.0 mg/L) to regeneration medium containing 6-benzyladenin (BA, 1.0...

Author(s):Tour Jan, Ali Hazrat ,Rashid Azam, Arshad Ali Mohammad Nisar, Nasrullah Khan and Muhammad Asif Nawaz

Article in Press

Bioremediation potentials of Sunflower (Tithonia diversifolia) and pseudomonas species in soil contaminated with lead and zinc.

This study was conducted to assess the ability of sunflower (Tithonia diversifolia) inoculated with Pseudomonas species to remediate soils contaminated with heavy metals (lead and zinc). The contaminants were added to 20 kg soil in pots as lead nitrate (Pb (NO3)2) and zinc nitrate (Zn (NO3)2); 400 mg/l metal concentrations were added to 20 kg soil in pots. The experimental design was a randomized complete block design...

Author(s):Elizabeth Temitope Alori, Abiodun Joseph, Ojo Timothy Vincent Adebiyi, Ajibola Patrick Aluko and Chidera Onyekankeya

Article in Press

Enhancement of somaclonal variations and genetic diversity using graphite nanoparticles (GtNPs) in sweet potato plants

To assay the efficiency of graphite nanoparticles (GtNPs) in sterilizing tissues and their role in enhancing genetic diversity, sweet potato is considered an important crop; hence its explants were used. In this experiment, GtNPs of 200, 400 and 800 ppm concentrations were used for sterilization of MS callus induction and regeneration media in Abees cultivar. The results showed that GtNPs had a good potential for removing...

Author(s):Aziza A. Aboulila, Ola A. Galal and M. F. M. El-Samahy

Article in Press

Technological properties and sugar tolerance performance of palm wine yeasts isolated from parts of Nsukka, Nigeria

The ability of yeasts to survive and produce significant ethanol in high sugar medium and high ethanol environment is essential for the use of such yeasts in industrial and edible ethanol production. Four Saccharomyces spp. strains ( ) isolated from palm wine and an industrial strain were studied in high glucose medium for ethanol production and ability to survive in high sugar medium. Saccharomyces cerevisiae produced...

Author(s):Nsikak U. Stephen, J. Obeta Ugwuanyi and Ukponobong E. Antia

Article in Press

Genetic diversity among elite chickpea (Cicer arietinum L.) varieties of Ethiopia based on inter simple sequence repeat markers

The inter simple sequence repeat (ISSR) marker was used in this study for genetic fingerprinting and identification of released chickpea varieties and for the determination of the genetic relationships among these cultivars. A total of 19 released chickpea varieties were subjected to ISSR analysis with the objective of evaluating the genetic diversity among the cultivars. A total of 20 ISSR primers were initially screened...

Author(s):Zerihun Jida, Shiferaw Alemu and Destaw Mullualem

Article in Press

Generation of electricity using watermelon waste (Citrullus lanatus) through a single chamber mediator-less microbial electrochemical cell

The use of fossil fuel for the generation of electricity pollutes the environment. The noxious gases that are expelled from it result in global warming just like the careless disposal of organic waste materials that destroy the aesthetic beauty of the environment. This research therefore aims to convert such organic wastes into electricity. The waste of watermelon fruit (Citrullus lanatus) collected from a fruit garden...

Author(s):Akpotayire, S. I., Kalagbor, I. A., Miikue-Yobe, T. F. B. and Nyiador, P.

Article in Press

In vitro inhibitory potentials of aqueous and ethanol extracts of Hyptis suaveolens on fungi associated with postharvest spoilage of Brassica oleracea

The inhibitory potential of Hyptis suaveolens leaf and root extracts on fungi associated with postharvest spoilage of cabbage (Brassica oleracea var. capitata) was examined. Collection of diseased cabbage was done in sterile sample bags. Isolation of fungi was carried out by inoculating small sections of diseased tissues excised from the cabbages unto Petri-dishes containing Acidified Potato Dextrose Agar (APDA). The...

Author(s):Chidiebere R. Agbawodike, Ayodele A. Sobowale, Jonathan S. Gbolagade and Ifeanyi Oscar N. Aguzie

Article in Press

Antibacterial saponins from the leaves of Polyscias fulva (Araliaceae)

Saponins are a major family of secondary metabolites that occur in a wide range of plant species. Bioassay - guided fractionation of extract of the leaves of Polyscias fulva led to the isolation of three known saponins named, 3-O-α-L-arabinopyranosyl-hederagenin (1), 3-O-[α-L-rhamnopyranosyl(1-2)-α-L-arabinopyranosyl]-hederagenin (2) and...

Author(s):Winnie Cherotich Maritim, Isabel Nyokabi Wagara and Josphat Clement Matasyoh

Article in Press

Genetic variability, heritability and genetic advance for green pod yield and yield related traits in chili (Capsicum annum L.) accessions in Ethiopia

A field experiment was conducted to study the genetic variability, heritability and genetic advance for 12 quantitative characters in chili (Capsicum annum L.) accessions in Ethiopia, during the off-season period of 2012/2013 under flood irrigation at the Adet Agricultural Research Centre field. The experiment was conducted using Randomized Complete Block Design with three replications and a total of 25 accessions and four...

Author(s):Tadesse Tilahun, Yohannes Petros and Yigzaw Dessalegn

Article in Press

Effects of temperature and time on oil extraction from some Nigerian indigenous fresh water microalgae species

This study determined the effects of temperature and time on oil extraction from indigenous freshwater microalgae: Dictyosphaerium, Chlorella, Desmodesmus and Cosmarium species, cultured in 15 2-L column photobioreactors (PBR) (three per species). Growth and specific growth rates for the mixotrophic>autotrophic cultivation of the species were determined. Oil was extracted from the dried microalgae species at temperatures...

Author(s):Felix Uzochukwu Asoiro, Wilfred Ifeanyi Okonkwo, Clement Onyeaghala Akubuo, Nkechinyere O. Nweze and Cosmas Ngozichukwu Anyanwu

Article in Press

Atomic absorption spectroscopic evaluation of some heavy metal contents in tomato (Lycopersicon esculentum Mill) fruit and Ribu River water used for irrigation at Fodugora Farm, Weliso Town, Ethiopia

The levels of some heavy metals in mature fruits of tomato (Lycopersicon esculentum Mill) grown at Fodugora Farm, Weliso Town (Ethiopia) with Ribu River effluent water, used for its irrigation, have been determined using flame atomic absorption spectroscopic (FAAS) technique. Whereas, the levels of Fe, Zn and Pb in tomato fruits were: 34.05, 4.05, and 1.32 mg/L respectively, their concentrations in the river effluent water...

Author(s):Gera Techane, O. P. Yadav and LokeshYadav

Article in Press

Microsatellites markers associated with resistance to flower bud thrips in a cowpea F2 population derived from genotypes TVU-123 and WC36

Breeding for resistance to flower bud thrips (Megalurothrips sjostedti) in cowpea has been hindered by the quantitative nature of resistance. To identify simple sequence repeat (SSR) associated with resistance to flower bud thrips that could be used for marker-assisted breeding, a F2 population was generated from a cross between genotypes TVU-123 (resistant) and WC36 (susceptible). The population was evaluated for thrips...

Author(s):Symphorien Agbahoungba Jeninah Karungi, Kassim Sadik, Paul Gibson, Richard Edema and Achille E. Assogbadjo

Article in Press

In vivo evaluation of aggressiveness, pathogenicity and patulin accumulation by Penicillium expansum strain isolated from Algerian apples

Penicillium expansum is one of the most important postharvest pathogens of apple fruit worldwide. It causes blue mold decay, one of the important economical diseases of apple fruits and it is the main producer of patulin. The main objective of this study is to evaluate the aggressiveness and patulin production of an isolated strain from apples produced in the East of Algeria. The strain was isolated, screened for its...

Author(s):Hamida KROUMA, Radia CHERFIA, Atef JAOUANI, Amor MOSBAH, Soukaina ABDELWAHAD, Mounira KARA ALI and Noreddine KACEM CHAOUCHE

Article in Press

Assessment of morphological characteristics among upland rice (Oryza sativa and Oryza glaberrima) germplasm

Rice is an important staple food crop that feeds over half of the global population and has become the cereal that provides a major source of calories for the urban and rural poor in Africa. This work aimed to evaluate the morphological of rice (Oryza sativa and Oryza glaberrima) germplasm. In the present study, 14 quantitative traits were used across 48 accessions or genotypes obtained from Central Agricultural Research...

Author(s):Zogbo Luther, Richard Akromah, David P. Tokpah and Zipporah Page

Article in Press

Molecular epidemiological studies on Trypanosoma evansi type A and type B in camels (Camelus dromedaries) from five different regions of Saudi Arabia using the ITS1 rDNA and RoTat 1.2 VSG gene

Trypanosoma evansi is the most widespread of the pathogenic salivarian trypanosomes and cause a serious disease called surra that affects domestic animals such as camels and horses in tropical and subtropical countries and often leads to reduced productivity and economic losses. Therefore, the objectives of the present study were to determine the prevalence rates of trypanosomiasis using polymerase chain reaction (PCR)...

Author(s):Abdullah D. Alanazi

Article in Press

Comparative survey of three processes used for the extraction of total phenol content and total flavonoid content of Anacardium occidentale L. and the assessment of its antioxidant activity

Three processes were used for the extraction of total phenol and total flavonoid contents of Anacardium occidentale L. and to determine its antioxidant activity. Whatever the extraction process was, the total flavonoid and total phenol contents were of the same order. With the microwave-assisted extraction (MAE), 666.07 mg gallic acid equivalent (GAE)/100 g dry matter (DM) of total phenol content was obtained from the stem...

Author(s):MADJITOLOUM BETOLOUM Salomon

Article in Press

Genetic diversity assessment of yams (Dioscorea spp.) from Ethiopia using inter simple sequence repeat (ISSR) markers

The genetic diversity and relationships of 70 accessions of yam belonging to Dioscorea cayenensis/Dioscorea rotundata complex (55), Dioscorea bulbifera (13) and Dioscorea alata (2, as a reference) were assessed using six inter simple sequence repeat (ISSR) primers. DNA was extracted from a bulk of two plants per accession using a modified cetyl trimethyl ammonium bromide (CTAB) method. Six ISSR primers amplified 77...

Author(s):Kedra Mohammed Ousmael

Article in Press

Study of secondary metabolites produced by Aspergillus flavus and evaluation of the antibacterial and antifungal activity

The objective of this study was the analysis of the secondary metabolite products and evaluation of antibacterial activity of Aspergillus flavus. Bioactives are chemical compounds often referred to as secondary metabolites. Thirty one bioactive compounds were identified in the methanolic extract of A. flavus. The identification of bioactive chemical compounds is based on the peak area, retention time, molecular...

Author(s):Ghaidaa J. Mohammed, Imad H. Hameed and Sabreen A. Kamal

Article in Press

Opaque-2 Gene and Beta Carotene Content in 20 Yellow-, Red and Purple-Kernelled Maize (Zea Mays L.) Accessions in Ghana.

Twenty yellow-, red- and purple-kernel maize accessions were collected from three geographic regions in Ghana for the study. The objective was to determine the presence of opaque -2 gene and -carotene content of the grains as a means of assessing nutritional quality. Variability in -carotene content was determined by HPLC while presence of opaque 2-gene was determined by a light box. Significant variations were found in...

Author(s):GILBERT ANSAH and KOJO AHIAKPA

Article in Press

Microbial machinery based synthesis of nanoparticulate hydroxyapatite using Serratia marcescens and its characterization for human bone implant

Hydroxyapatite (HAp), chemically and compositionally similar to the material presents in bone and has a unique biocompatibility which makes it an attractive material for bone implant. We aimed at studying the physical and chemical properties of HAp synthesized by Serratia sp. The total of 58 strains was isolated from 354 samples obtained from clinical and environmental sources like urine, sputum, soil, water and plant...

Author(s):Rathinasamy Subashkumar, MIDHUSHA JOHNY and Nandhagopal Gayathri

Article in Press

Fatty acids profiling of the seed oils of some varieties of peanut (A.hypogaea L.) towards the development by GC-MS analysis.

Twelve fatty acids were identified during maturity in the wild Tunisian and cultivar peanut kernels. These fatty acids include C16:0 (palmitic acid), C18:0 (stearic acid), C18:1 (oleic acid), C18:2 (linoleic acid), C19:0 (nonadecanoic acid), C20:1 (gadoleic acid), C20:0 (arachidic acid), C22:1 (erucic acid), C22:0 (behenic acid), C23:0 (tricosanoic acid), C24:0 (linoceric acid), and C26:0 (cerotic acid). Three fatty acids...

Author(s):Aicha. O. CHERIF, Mhamed BEN MESSAOUDA, Habib KALLEL, Claude PEPE

Article in Press

Isolation of a novel strain Bacillus sp.in producing neutral and heat stable beta-1,4 endoglucanase activity

Neutral a β-1-4 - endoglucanase was produced by aerobic bacteria a newly strain Bacillus sp .RL. Isolated from soil, Chong Qing province, China. In the present study the bacteria produce a significant amount of enzymes after 72 hrs of fermentation CMC medium were 0.1852 U/ml of endoglucanase, 0.5814 U/ml of FPase activity and 2.9940 mg/ml of extracellular protein. These strains showed heavy growth and liquefaction of 7.0,...

Author(s):Rahman Laibi Chelab, Dan Dan Wang and Xingyong Yang

Article in Press

In vitro propagation as a viable alternative to the continuous regeneration of three Eucalyptus species (Eucalyptus camaldulensis, E. gomphocephala and E. maidenii)

The objective of this work was to propagate three species of Eucalyptus: Eucalyptus camaldulensis, Eucalyptus gomphocephala and Eucalyptus maideni on a large scale using microcutting technique. For the test of microcutting, from in vitro germination, the best rate of multiplication of 4.75 were obtained for E. camaldulensis on a medium containing 1 mg/L 6-benzylaminopurine (BAP) and 1 mg/L -aminoisobutyric acid (AIB) in...

Author(s):Hamrouni Lamia, Hanana Mohsen, Derbali Fedia, Hwass Haifa , Khouja Mohamed Larbi and Bouzid Sadok

Article in Press

Cloning and expression of recombinant human insulin gene in Pichia pastoris

The main goal of this current study was the cloning and expression of the human insulin gene in Pichia pastoris expression system, using genetic engineering techniques and its treatment application. Total RNA was purified from fresh normal human pancreatic tissue. RNA of good quality was chosen to obtain a first single strand cDNA. Human preproinsulin gene was amplified from cDNA strand using two sets of specific primers...

Author(s):Rafid A. Abdulkareem, Samah A. Jassam and Majid J. Jabber

Article in Press

Genetic diversity of antibiotic and plant extract resistant Staphylococcus aureus isolated from hospitalized patients in Ekiti State, Nigeria

This study aimed to determine the genetic diversity of antibiotics and plants extract resistant Staphylococcus aureus using molecular technique. A total of 106 human clinical samples were collected from patients in three different hospitals within Ekiti State. Microbiological and molecular analyses were performed using standard methods. Antimicrobial susceptibility test with common antibiotics and plant leaves extracts was...

Author(s):Egbebi Adeola Oluwagbemileke, Oyama Maslhanty Obem and Fadare Olalekan Shadrach

Article in Press

Physico-chemical properties of Brazilian biomass: Potential applications as renewable energy source

Biomass thermal behavior under different atmosphere: combustion, pyrolysis (N2) and typical oxy-fuel combustion (60 and 80% CO2) were investigated by thermal analysis (TG/DTG and DTA). Other techniques such as X-Ray, Fourier Transformed Infrared (FTIR), Wettability, Scanning Electron Microscopy (SEM) and Inductive Couple Plasma - Optical Emission Spectrometer (ICP-OES) were also used to characterize the samples. Five...

Author(s):Glauber CRUZ, Carlos E. M. BRAZ, Ivonete ÁVILA and Paula M. CRNKOVIC

Article in Press

Establishment of high frequency regeneration protocol for Lycopersicon esculentum from leaf explant using seaweed extracts

The ultimate aim of this study is to standardize the conditions for in vitro organogenesis of tomato using plant growth regulators, organic elicitors, polyamines and seaweed extracts. Initially excised leaf explant was cultured on to the medium containing different concentrations of auxins. The best responded auxin was tested in combination with efficient cytokinins (Kinetin [Kin], 6-Benzylaminopurine [BAP] and Thidiazuron...

Author(s):S. Vinoth, P. Gurusaravanan, S. Sivakumar, G. Siva, G. Premkumar and N. Jayabalan

Article in Press

Characterization of lactic acid bacteria isolated from dromedary raw milk in Syria

Lactic acid bacteria (LAB) are considered a micro flora of Syrian camel milk. A total of 235 isolates of LAB were obtained from 63 camel milk samples, which have been collected from producing areas of different regions of Syria during 2009 and 2012. The isolates were cocci and rods, gram-positive and catalase-negative. 132 isolates were identified as cocci including 76 isolates belonging to Enterococcus (45 were...

Author(s):Ayman Al-Mariri, Ahed Abou Younes and Laila AL-Hallab

Article in Press

In silico analysis of antimicrobial eupalitin isolated from Ageratina adenophora using docking method

The present study evaluated the efficacy of eupalitin compounds against the target protein, dihydrofolate reductase of bacterial and fungal pathogens using in silico approach. Structure based docking studies was performed using Schrodinger (Version: 1.8) tool to retrieve potential candidates with high affinities binding against eupalitin models with several targeted proteins repositories. The specific targets were selected...

Author(s):Suganya Mani, Kavitha Shivaji, Karthik Natesan, Ashaq Hussain Bhat, Mythili Gnanamangai Balasubramanian and Ponmurugan Ponnusamy

Article in Press

In vitro screening of traditional rice varieties of Assam, India for drought tolerance

Eight traditional rice varieties viz., bora, prosad bhog, kola joha, helash bora, salihoi bao, kola amona, ronga bora and sok-bonglong along with ‘sahbhagi dhan’ (drought tolerant) and ‘IR64’ (drought sensitive) were studied invitro under two different drought stress conditions. Different parameters studied were germination percentage, shoot length and root length. Drought stress was produced by ½ Murashige and Skoog (MS)...

Author(s):Shamsun Nahar and Bhaben Tanti

Article in Press

Potential application of nutrigenomics in livestock: Lesson from developed countries

Livestock performance is affected by genotype and environment. Nutrition is one of the greatest environmental determinants. Genes function according to the metabolic signals received by the nucleus from hormones and nutrients. Currently, nutrigenomics, which encompasses the three crucial omics stages (transcriptomics, proteomics and metabolomics), is emerging in the area of livestock to develop feeds that can be...

Author(s):Bruh Asmelash Gebremikael, Mahlet Dawit Teweldebrihan and Haftom Brhane Mesfin

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