African Journal of
Biotechnology

  • Abbreviation: Afr. J. Biotechnol.
  • Language: English
  • ISSN: 1684-5315
  • DOI: 10.5897/AJB
  • Start Year: 2002
  • Published Articles: 12481

Full Length Research Paper

TEM-145 and TEM-146 β-lactamases produced by Escherichia coli isolates from state hospitals in KwaZulu-Natal, South Africa

Chunderika Mocktar1*, Usha Govinden1, Adrian Willem Sturm2 and Sabiha Essack1
1School of Pharmacy and Pharmacology, Faculty of Health Sciences, University of KwaZulu-Natal, Private Bag X54001, Durban, 4000, South Africa. 2Department of Medical Microbiology, Nelson R. Mandela Medical School, University of KwaZulu-Natal, South Africa.
Email: [email protected]

  •  Accepted: 04 January 2007
  •  Published: 05 March 2007

Abstract

Two Escherichia coli isolates which were isolated from the urine of patients in state hospitals in KwaZulu-Natal, South Africa were investigated to determine the sequence of the TEM β-lactamases responsible for their resistance to β-lactamase inhibitors. The isolates were subjected to MIC determinations, iso-electric focusing analysis, plasmid analysis, polymerase chain reaction (PCR) for the detection of β-lactamase genes and sequencing of the blaTEM. Analysis of the nucleotide sequences revealed the presence of two novel TEM β-lactamases, TEM-145 and TEM-146 which had the R244H mutation. Mutations at position 244 have been previously reported in other inhibitor-resistant TEMs (IRTs). 

 

Key words:  Escherichia coli, plasmid-mediated, TEM β-lactamase.

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This article is published under the terms of the Creative Commons Attribution License 4.0

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