African Journal of
Biotechnology

  • Abbreviation: Afr. J. Biotechnol.
  • Language: English
  • ISSN: 1684-5315
  • DOI: 10.5897/AJB
  • Start Year: 2002
  • Published Articles: 12481

Full Length Research Paper

Preliminary molecular analysis of the genetic diversity of some Atriplex species present in the Northeast of Algerian

MÂALEM Souhaïl1,3*, KHOUFI Sahari2, RAHMOUNE Chaâbane3 and BENNACEUR M’Barek2
1Department of Life Sciences and Nature, Exact Sciences and Life Sciences and Nature Faculty, Tebessa University, 12000 Tébessa, Algeria. 2Laboratory of Plant Physiology and Biotechnology, National Institute of Agronomic Research of Tunisia (INRAT) Street Hedi Karray, 2080 Ariana, Tunisia. 3Laboratory of Ecotoxicology and Abiotic Stresses, Sciences life and nature Department, Sciences Faculty, Constantine Mentouri University, Road Ain el Bey, 25000 Constantine, Algeria.
Email: [email protected]

  •  Accepted: 11 May 2012
  •  Published: 29 May 2012

Abstract

In the arid and semi arid areas, salt bush (Atriplex) represents an important forage resource. The characterization of the genetic diversity of these species is useful for their classification, their conservation and their improvement. In this context, we used the random amplified polymorphic DNA-polymerase chain reaction (RAPD-PCR) technique to test the genotypes of three different species of AtriplexAtriplex halimus,Atriplex canescens and Atriplex nummularia. The Obtained results showed 319 amplified bands which were mostly polymorphic. The analysis of the results generated four different groups where the genotypes of two introduced species (A. canescens and A. nummularia) were clustered into two independent groups. However, genotypes of the autochthonous species (A. halimus) was subdivided into two different independent groups where we noted that individuals of one of these groups are genetically the nearest between them, by report, to the existing others within the same group. In general, results reveal inter and intra high level of genetic diversity in the Atriplex studied species.

 

Key words: AtriplexChenopodiaceae, genetic diversity, molecular analysis, steppe, Algeria.