African Journal of
Biotechnology

  • Abbreviation: Afr. J. Biotechnol.
  • Language: English
  • ISSN: 1684-5315
  • DOI: 10.5897/AJB
  • Start Year: 2002
  • Published Articles: 12481

Full Length Research Paper

Effect of sodium benzoate on the growth and enzyme activity of Aspergillus niger and Penicillium citrinum in Zobo drink during storage at 30 ± 2°C

O. E. Nwafor1* and M. J. Ikenebomeh2
1Department of Microbiology, Delta State University, Abraka, Nigeria. 2University of Benin, Benin City, Nigeria.
Email: [email protected]

  •  Accepted: 13 March 2009
  •  Published: 17 June 2009

Abstract

The effect of different concentrations of sodium benzoate on the growth and enzyme elaboration potentials of Aspergillus niger and Penicillium citrinum in zobo drink packaged in glass bottles were investigated. All the tested concentrations of 0.025, 0.05 and 0.075% (w/v) caused decreases in the counts of A. niger and P. citrinum at 30 ± 2°C. Concentration of 0.05% (w/v) of the preservative hurdle showed complete inhibition of A. niger in the 4th week whereas the same effect was observed in the 5th week on P. citrinum. Enzyme activity in the control experiments was significantly higher (P < 0.05) than those of treated and fresh Zobo samples.

Key words: Sodium benzoate, enzyme activity, Zobo drink.