Abstract

Yeast of the genus Candida has been recognized as important microorganisms responsible for Nosocomial fungemia. Six isolates of clinical Candida albicansisolated from patients and studied by electrophoretic karyotyping of chromosomal DNA by pulsed field gel electrophoresis. Six chromosal DNA profiles were more or less identical. Natural fungicide nystatin is more effective on C. albicans growth than the other synthesitic miconazole nitrate and clotrimazole. The minimum inhibitory concentration for ≈ 90% growth of C. albicans (MIC90) of Nystatin is1500 unit/ml. The antimicrobial activities of alternative and traditional medicine in Saudi Arabia were evaluated against C. albicans growth. Natural acetic acid, plant extract, charcoal and Elshab are used as natural drug for Candidiasis. The percent of 36% Concentration of acetic acid in media causes maximum inhibition of C. albicans growth. There are direct relation between acetic acid concentrations and inhibition zones. Increase or decrease of pH inhibits the growth of C. albicans. The ideal pH for inhibiting C. albicans growth is pH 10. The combination of Salvadora persica extract (Meswak) and Elshab (KAl(SO₄)₂) in concentration of (28.5 + 12.75 mg/ml, respectively), have an antimicrobial effect on C. albicans growth (0.01 g/10ml of media). Charcoal in 20 mg/ml concentration or Lawsonia alba extract (Henna) in 250 mg/ml is the ideal concentration used for stop growth of C. albicans. The susceptibility of yeast to the previous alternative medicine shows that, it could be used in treating candidiasis resulting from different strains of C. albicans instead of commercial antibiotics used, without any adverse side effects. Also, it is an attempt to stop development of multidrug resistance organism.Aspergillus fumigatus performed a good tool as biocontrol for C. albicans which caused infections mainly candidiasis. Both are isolated as clinical isolates ofnosocomial of infected patients.

Key words: Candida albicans, chromosomal DNA, Lawsonia alba, Salvadora persica, antibiotics, biocontrol.