May 2003
Bioinformatic tools and guideline for PCR primer design
Bioinformatics has become an essential tool not only for basic research but also for applied research in biotechnology and biomedical sciences. Optimal primer sequence and appropriate primer concentration are essential for maximal specificity and efficiency of PCR. A poorly designed primer can result in little or no product due to non-specific amplification and/or primer-dimer formation, which can become...
May 2003
Substrate Channelling and Energetics of Saccharomyces cerevisiae DSM 2155 Grown on Glucose in Fed-Batch Fermentation Process
Data collected during the high-cell-density cultivation of Saccharomyces cerevisiaeDSM 2155 on glucose in a simulated five-phase feeding strategy of fed-batch process, executed on the Universal BIoprocess CONtrol (UBICON) system using 150L bioreactor over a period of 24h have been analysed. The consistency of the data set was checked using both the available electron and carbon balances. Estimates...
May 2003
Biodegradation of Bonny light crude oil in soil microcosm by some bacterial strains isolated from crude oil flow stations saver pits in Nigeria
In an effort at developing an active indigenous bacterial consortium that could be of relevance in bioremediation of petroleum contaminated systems in Nigeria, four hydrocarbon degrading bacteria strains were isolated. Partial sequencing of the 16S rDNA of the isolates suggests that they are all strains of Pseudomonas aeruginosa. Axenic cultures of the isolates biodegraded Bonny light crude oil in...
May 2003
Genetic affinities of Fusarim spp. and their correlation with origin and pathogenicity
Random amplified polymorphic DNA (RAPD) analyses was used in combination with pathogenicity assays to study the taxonomic kinships among five Fusarium species. A total of 46 isolates of Fusarium spp. obtained from diseased cotton seedlings showing typical root rot and dampping-off symptoms were characterized. Of 10 primers tested, four primers produced polymorphic amplification...
May 2003
Four gene introduction methods affect the shoot regeneration and localization of transgene expression in greenhouse stem explants and in vitro-grown chrysanthemum stem thin cell layers
Gene introduction method (GIM) affected shoot regeneration capacity (SRC) in standard and spray-type chrysanthemums. SRC was both cultivar and GIM-dependent in both in vitro and greenhouse stem explants, the former significantly higher than the latter. Sonication had an SRC-stimulating effect on in vitro explants. Other GIMs (Agrobacterium, biolistics, Agrolistics) had an...
May 2003
Molecular distribution of gypsy-like retrotransposons in cotton Gossypium Spp.
PCR primers specific for conserved domains of the reverse transcriptase (RT) genes ofgypsy-like retrotransposons amplified their corresponding gene in two Gossypium barbadense cultivars. Analysis with the FASTA software showed a high DNA sequence homology to pine, gypsy LTR-retrotransposon. Using the PCR product as a hybridization probe, gypsy-like retrotransposons were detected in...
May 2003
DNA Sequences of RAPD Fragments in the Egyptian cotton Gossypium barbadense
Random Amplified Polymorphic DNAs (RAPDs) is a DNA polymorphism assay based on the amplification of random DNA segments with single primers of arbitrary nucleotide sequence. Despite the fact that the RAPD technique has become a very powerful tool and has found use in numerous applications, yet, the nature of molecular variation(s) uncovered by the RAPD technique is still unclear. The aim of the following...
May 2003
Effect of salt and drought stress on acid phosphatase activities in alfalfa (Medicago sativa L.) explants under in vitro culture
Acid phosphatase is wildly found in plants. This enzyme has intra and extra cellular activity. For instance, it dephosphorylase organic phosphate and change it to inorganic phosphate. However, acid phosphatase activity is increased by salt and osmotic stress. In this experiments, calluses were produced from invitro grown explants of Medicagosativa cv. Yazdi and cv. Hamedani under aseptic...
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