Scientific Research and Essays

  • Abbreviation: Sci. Res. Essays
  • Language: English
  • ISSN: 1992-2248
  • DOI: 10.5897/SRE
  • Start Year: 2006
  • Published Articles: 2768

Full Length Research Paper

Microsatellite gene diversity within Philippines dwarf coconut palm (Cocos nucifera L.) resources at Port-Bouët, Côte d’Ivoire

Konan K. Jean Noël1*, Koffi K. Edmond2, Konan K. J. L.3 and Konan K.Eugene1
  1Station de recherche de La Mé, Centre National de Recherche Agronomique (CNRA), 01 BP 989 Abidjan 01, Côte d’Ivoire. 2Laboratoire Central de Biotechnologies (LCB). Centre National de Recherche Agronomique (CNRA), 01 BP 1740 Abidjan 01, Côte d'Ivoire. 3Station de Recherche de Port Bouët Marc Delorme, Centre National de Recherche Agronomique (CNRA),  07 BP 13 Abidjan 07, Côte d’Ivoire.
Email: [email protected], [email protected]

  •  Accepted: 18 October 2011
  •  Published: 23 November 2011

Abstract

 

Description and assessment of genetic resources are common preoccupations for their better management and utilization in breeding program. Progress made to date is still limited in view of assessment of coconut resources in the International Coconut Genebank for Africa and Indian Ocean (ICG-AIO) at Marc Delorme Port-bouët experimental station in Côte d’Ivoire. Using twelve microsatellite markers, weanalyzed genetic diversity and genetic relationship among 25 palms representing five Philippines coconut green dwarf accessions. The 12 primers yielded 40 alleles, with an average of 3.33 alleles per primer. The within-accession genetic diversity (He) ranged from 0.067 ± 0.159 to 0.325 ± 0.205. In spite of the low level of polymorphism observed at microsatellite loci, factorial analysis of correspondence and hierarchical cluster analysis showed a clear distinction of aromatic green dwarf accession from others Philippines green dwarf accessions, which were not clearly separated. Indeed, there is no apparent partition between Kinabalan green dwarf and Catigan green dwarf individuals and between Pilipog green dwarf and Tacunan green dwarf accessions. Nevertheless, genetic differentiation indices ranged from 0.165 to 0.47 were obtained between these last accessions. The opportunity to consider these accessions as distinct each other relatively to their low genetic differentiation indices was discussed.

 

Keys words: Assessment, dwarf coconut accessions, microsatellites, Côte d’Ivoire.