Bovine viral diarrhea (BVD) is one of the most economically important diseases of cattle population worldwide and caused by bovine viral diarrhea virus (BVDV). A cross-sectional study was conducted from October, 2019 to April, 2020 to investigate the sero-prevalence and associated risk factors for Bovine Viral Diarrhea Virus (BVDV) infection in and around Asella town, Ethiopia. Semi structured questionnaire survey was designed to assess the different variables related to herd managements through a personal interview of the farmers. Serum samples were collected from a total of 45 non- vaccinated cattle herds (225 individual cattle) during the study period. Samples were examined for detection of bovine viral diarrhea virus (BVDV) antibodies using an indirect enzyme- linked immunosorbent assay (i-ELISA) kit (ID Screen® BVD p80 Antibody) following the manufacturer protocol. Chi-square analysis and multivariable logistic regression model were used to identify risk factors for BVDV seropositivity. From the 225 tested serum samples of cattle, 19 (8.4%) (95% CI: 5.2–12.9) were positive for antibodies against BVDV using the indirect antibody ELISA technique. The present study demonstrated that BVDV infection was less spread in dairy herds in and around Asella town, Ethiopia.

Keywords: Bovine Viral Diarrhea Virus, Seroprevalence, Indirect ELISA, Ethiopia