Picralima nitida is a medicinal plant of the family Apocynaceae, frequently used in South- eastern Nigerian traditional medicine for its therapeutic properties. This study is a comparative evaluation on the chemical constituents and bioactivity of Picralima nitida seed, leaf, root and stem back methanol extracts. The phytoconstituents present were identified using GC-MS (Gas Chromatography-Mass Spectrometry) analysis. The GC-MS analysis revealed twenty eight bioactive compounds in P.nitida seed methanol extract, with Hexadecane, 2,6,10,14-tetramethyl- ( C20H42) and o-Terphenyl (C18H14) having the highest peak of 10.32 and 9.36% respectively. Twenty seven bioactive compounds in P.nitida leaf exract, with Hexadecane, 2,6,10,14-tetramethyl ( C20H42) and o-Terphenyl ( C18H14) having the highest peak of 10.48 and 8.90 respectively. Thirteen bioactive compounds in P.nitida stemback methanol extract with 3,8-dimethyl-4-(1-methylethylidene)-, (8S-cis)- and 1-(2-Methoxyphenyl)-2,5-dihydro-1H-pyrrole-2,5-dione having 82.64 and 5.92% peak and nine bioactive compounds in P.nitida root extract with Coumarin, 7-hydroxy-4-methyl-3-propyl- (C13H14O3) and β-Amyrin trimethylsilyl ether (C33H58OSi) having 65.89 and 24.02% respectively. Findings showed that P.nitida seed contains more bioactive compounds followed by the leaf, stem back and seed while the stem back and root extract has the highest percentage peak. The results concludes that these bioactive compounds may have lends credence to its ethnomedicinal uses and therefore suggests that synergistic utilization at required dose of this plant extract should be encouraged in order to holistically maximise the medicinal potentials of this plant.

Keywords: Picralima nitida, bioactive , constituents, comparative