Hyper pigmentation is skin disorders affect the color of skin that may require treatment. There are many chemical agents to treatment of hyper pigmentation such as hydroquinone, retinoids and other agents but do not satisfactory results and may be highly morbid. In addition to consumer prospective natural origin is more acceptable with mush less contraindication. Soybean derived peptides have demonstrated numerous dermatological benefits such as treatment of hyper pigmentation, photo aging disorders and skin cancer. The aim of this investigation is to develop and evaluation of protective formulations [cream] containing soybean derived peptides as reducing skin pigmentation and increasing consumer compliance. Therefore the soybean derived peptides were extracted and filtered through amicon filter to cut off 30KD. The peptides extracted were characterized by SDS-PAGE methods and then determination by Bradford method. Afterward were formulated in cream dosage form and evaluated for pharmaceuticals parameters including organoleptic characteristics, pH, heat stability, stability, centrifugal test, rheological characteristic, in vitro drug release and drug content. The results of this study suggest that the formulated cream containing of soybean derived peptides were stable for the six months under the temperature and storage condition (250C). The in vitro release rate of formulation was found to obey Higuchi Kinetic model. According to Table5 drug content was changed from 98.07% to 88.8% after storage at room temperature (250C) for six months.

Keywords: Hyper pigmentation, SDS-PAGE methods, Bradford assay