Abstract

This study is planned to determine the antioxidant activity. Indian medicinal plants were selected to evaluate for free radical scavenging capacities and antioxidant activities using commonly accepted assays. They were extracted with ethanol to show the best antioxidant results. Flavonoids and phenolic were included and used as standards in this study. Each sample under assay condition showed a dose-dependent free radical scavenging effect of DPPH (1, 1-diphenyl-2-picryl hydrazyl radical) and a dose-dependent inhibitory effect of lipid peroxidation. Among plant extract of Commiphora species in family Burseraceae that isCommiphora berryi, C. caudata and C. pubescens, the antioxidant properties of three different species of Commiphora were formulated to polyherbal tablets and the in-vitrostudies are evaluated by different methods like phosphomolybdate method, nitric oxide scavenging activity, superoxide anion radical scavenging activity, scavenging of hydrogen peroxide and lipid peroxidation inhibition. A positive result was observed in the antioxidant activity potential between the total flavonoid and phenolic content assay. The total phenolic contents following order EECB > EECP > EECC and PHTF-II ^~ PHT F-I respectively. Flavonoid content and other activities can be performed by ethanolic crude extracts of poly herbal tablet formulation. The entire sample respectively when analyzed at a concentration of 1000 µg/ml.

Key words: Commiphora berryi, Commiphora caudata, Commiphora pubescens, anti oxidant acvity, in vitro studies

Abbreviation

DPPH, - 2, 2-diphenyl-1-picrylhydra-zyl; RSC, radical scavenging capacity;O₂, superoxide; H₂O₂, hydrogen peroxide; OH, hydroxyl; FeCl₃, ferric chloride; FeCl₂,ferrous chloride; Ao, absorbance of the control; A₁, absorbance of crude extracts and standards, T, sample; C, vehicle control; B, zero time control; CB, commiphora berryi; CC,commiphora caudate; CP, commiphora pubiscens; PHT, poly herbal tablet; PHF; poly herbal formulation.