Journal of
Medicinal Plants Research

  • Abbreviation: J. Med. Plants Res.
  • Language: English
  • ISSN: 1996-0875
  • DOI: 10.5897/JMPR
  • Start Year: 2007
  • Published Articles: 3831

Full Length Research Paper

High performance liquid chromatographic analysis of derivatized sapogenin of Asparagus (RP-HPLC analysis of derivatized sapogenin of Asparagus)

Jagmohan S. Negi1*, Pramod Singh2, Geeta Joshi nee Pant2 and M. S. M. Rawat2
1Herbal Research and Development Institute, Mandal-Gopeshwar (Chamoli)-246401, Uttarakhand, India. 2Department of Chemistry, HNB Garhwal University, Srinagar (Garhwal)-246 174, Uttarakhand, India.
Email: [email protected], [email protected]

  •  Accepted: 28 September 2010
  •  Published: 18 May 2011

Abstract

RP-HPLC method on C8 column was investigated for derivatized sapogenin, the marker inAsparagus species using water and acetonitrile as mobile phase in isocratic manner at a flow rate of 0.5 ml/min. The method is sensitive (LOD = 2.02 µg), accurate (average recovery was 98.97%) and precise (intra day variation of RT<0.398, inter day variation of RT<0.982). Quantification was done by UV absorption at 210 nm. Rhizome has maximum concentration of bioactive ranging in between 0.10 to 3.72.

 

Key words: Asparagus racemosus, A. curillus, RP-HPLC, sarsasapogenin, acetyl sarsasapogenin, quantification.

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