The effect of bio-inoculants and PGRs on germination and seedling growth of wild Ber (Ziziphus rotundifolia Lamk.) under laboratory condition was conducted in the Department of Seed Science and Technology, CCS Haryana Agricultural University, Hisar during the session 2021-22. The seeds removed from stone were treated with five bio-inoculants viz, Azoteeka- Mac 27, Azoteeka- HT 54, Phosphoteeka- P36, Azospirillum, integrated biofertilizer by soaking for 30 min and dipped in six PGRs viz., GA3 @ 50 ppm, GA3 @ 100 ppm, NAA @ 50 ppm, NAA @ 100 ppm, IBA @ 50 ppm, IBA @ 100 ppm for 24 hrs and control. Among different treatments under laboratory conditions, maximum germination percentage (80.44%), maximum vigour indices (vigour index- I (1633), vigour index- II (2762)) and the least mean germination time (9.75 days) was recorded when seeds were treated with GA3 @ 100 ppm for 24 hrs. Among different bioinoculant treatments, the seed treated with integrated biofertilizer for 30 min led to the highest seedling length (20.33 cm) as well as the maximum seedling dry weight (35.06 mg).

Keywords: Bio-inoculants, Plant growth regulator, Seed germination, Seedling growth, Wild Ber .