Asymptomatic bacteriuria among healthy female students is a common occurrence that is frequently ignored and this is attributed to the fact that pre-menopausal, non-pregnant women with asymptomatic bacteriuria experience no adverse effects and usually will clear their bacteriuria spontaneously. However, these women are more likely to experience subsequent symptomatic urinary tract infection (UTI) than women who do not have asymptomatic bacteriuria. The aim of this study is to determine the incidence of asymptomatic bacteriuria among female students of Ahmadu Bello University (A.B.U), Zaria Main Campus. A total of 400 midstream clean-catch urine samples were analyzed using standard microbiological methods. Bacteriological analysis of the urine samples revealed a 16% (63/400) asymptomatic bacteriuria among female students in A.B.U main campus with a total of 148 bacteria isolates. The most prevalent bacteria were Klebsiella spp. and Acinetobacter spp. (19.59%), followed by Enterobacter spp. (17.57%) and Escherichia spp. (11.49%). A high incidence of resistance to Tetracycline (74%) and Ceftriaxone (78%) was observed. This study showed that there is an incidence of asymptomatic bacteriuria among healthy female students in A.B.U main campus.