Full Length Research Paper
Abstract
During nucleotide sequences of 16SrDNA gene, Enterococcus faecalis was predominant in 322 samples; 11 isolates (100%) were from outpatients' vaginal swabs and 3 isolates (75%) from inpatients' blood samples. The three isolates from the blood showed two frame shift mutations "Deletion" at sequence positions 21 and 1076 bp to be 100% similar with type strain OG1RF. The isolates totally showed no resistance to gentamycin, streptomycin and erythromycin whereas, there was high resistance to amoxicillin/clavulanic acid (71.4%), methicillin and vancomycin (92.8%, for each). All isolates from the blood were completely resistant to penicillin and ampicillin. The study also detected Planomicrobium mcmeekinii from an inpatients' blood case (containing Gene or Point mutation type Transversion at position 358 bp resulting to 99% sequence identity to ATCC 700539) as first reporting and revealing it to be a new subspecies or species.
Key words: Enterococcus faecalis, Planomicrobium mcmeekinii, 16SrDNA and sequencing.
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