Abstract

In this research characterization and method for determination of CGA in green and roasted coffee beans have been reported by UV-Vis spectroscopy. The optical transition properties and solvent effects of CGA were measured in different polar-solvents. After characterization of the electron transition of the standard solutions, a method was developed for UV-Vis spectrophotometer determination of CGA in coffee beans. The method was resolved (deconvoluted), the overlapped spectra of CGA and caffeine using dichloromethane and a direct determination of CGA from the residue of the extraction, at peak height. The validations of the methods are limit of detection, limit of quantification, linearity, recovery study and precision calculated as coefficients of variation. The obtained results were in excellent agreement with those obtained with HPLC method, verified by the student t-test at 95% confidence level.

Key words: Coffee, CGA, UV-Vis spectroscopy, extraction, optical transition properties, solvent effects.