Abstract

Our study focused on assessing frequency of Klebsiella oxytoca, to study incidence ofK. oxytoca producing ESBLs, to investigate frequency of blaTEM, blaSHV and blaCTX-M and to determine resistance of K. oxytoca producing ESBLs toward non-beta-lactam antibiotics, in different seasons in Ilam hospital. Twelve clinical isolates of K. oxytoca were found during March 2007 to April 2008 in Ilam hospital in Ilam city in west of Iran. The strains were isolated from admitted patients in surgery wards, lesion and respiratory tract infection. ESBLs identified by phenotypic and genotypic methods. PCR had done for detection of blaSHV, TEM and CTX-M. K. oxytoca producing ESBLS were evaluated against non-beta-lactam antibiotics of twelve K. oxytoca collected in Ilam hospitals, 16.67% (n=2), 16.67% (n=2) and 66.66% (n=8) were from the surgery wards, lesion, and respiratory tract infections (RTIs), respectively. 25% of K. oxytocawere ESBLs positive. blaSHV were found as responsible for ESBLs production. All theK. oxytoca producing ESBLs were susceptible to non-beta-lactam antibiotics. The highest frequency of K. oxytoca were found from patients with RTI (66.7%) and the lowest frequency of K. oxytoca had observed in admitted patients in surgery ward and patients with lesion infections, as an equal (16.6%). Our finding showed that resistant to ceftazidime was more than the others antibiotics (41.66%). We were found the most ESBLs production occurred in winter in K. oxytoca isolated in surgery ward (50%).

Key word: K. oxytoca, ESBLs, Ilam Hospitals, Iran..