Abstract

The unsatisfactory results obtained in the treatment of severe acute pancreatitis or the "great abdominal drama"; with pancreatic and peripancreatic infected necrosis require new therapeutic methods. This paper aims to evaluate the antimicrobial effect of ozonated saline on bacterial and yeast species commonly involved in pancreatic and peripancreatic necrosis infection in severe acute pancreatitis. Tests were conducted on four bacterial species (Staphylococcus aureus ATCC 6538P, Escherichia coli ATCC 10536, Klebsiella pneumoniae and Pseudomonas aeruginosa ATCC 27853) and one Candida albicansATCC 90028 strain. Ozonated saline and Imipenem (antibiotic) was used for all microbial strains. Two types of tests were used: the first on solid medium to determine the number of colonies (CFU/mL) and the second type of tests was the liquid medium for MIC determination. The results show that ozonated saline caused a reduction in the number of bacteria and yeast colonies even in small concentrations in plates. Minimum inhibitory concentrations obtained were very small for the strains of S. aureus ATCC 6538P (0.001563 mg/mL) and E. coli ATCC 10536 (0.003125 mg/mL) and; somewhat higher forK. pneumoniae (0.05 mg/mL), P. aeruginosa ATCC 27853 (0.1 mg/ml) and C. albicans. These results are encouraging in terms of possible use by peritoneal lavage of ozonated saline for pancreatic necrosis infection in acute pancreatitis.

Key words: Ozonated saline, antimicrobial effect, acute pancreatitis.