June 2010

Effects of diet-induced hypercholesterolemia on the lipid profile and some enzyme activities in female Wistar rats

The effects of a high dietary soybean oil and cholesterol on serum total cholesterol, low density lipoprotein (LDL-C), high density lipoprotein (HDL-C), and triglycerides were investigated. Total protein, albumin, glucose, gamma glutamyl transferase (GGT), aspartate aminotransferase (AST) and alanine amino transferase (ALT) activities were also investigated in weanling female Wistar rats for eight weeks. Two groups of...

Author(s): Gloria A. Otunola, Oyelola B. Oloyede, Adenike T. Oladiji and Anthony A. Afolayan

June 2010

Malate dehydrogenase from blood stream Trypanosoma vivax exists in isoenzyme forms

Malate dehydrogenase was isolated and partially purified from blood streamTrypanosoma vivax. The enzyme exists in isoenzyme forms (MDH1 and MDH2). The relative molecular weights of MDH1 and MDH2 determined by Sodium dodecyl sulphate polyacrylamide gel electrophoresis were 61 and 63 kdal. respectively. MDH2 lost activity immediately after purification. It has purification fold of 15 and percentage...

Author(s): A. U. Wurochekke, A. J. Nok, H. M. Inuwa and C. E. Gimba

June 2010

Purification and characterization of the α-amylase isolated from Penicillium camemberti PL21

The amylase family of enzymes is a great significance due to its wide area of potential application. α-amylase from Penicillium camemberti PL21 obtained from I. N. A. (The technological laboratory of Agriculture National Institute Paris- France) and using orange waste as substrates was produced under optimum conditions, after 168 h of incubation and subjected for purification and characterization. The...

Author(s): Tahar Nouadri, Zahia Meraihi, Djekrif-Dakhmouche Shahrazed and Bennamoun Leila

June 2010

The distribution of cyanide detoxifying enzymes (rhodanese and 3- mercaptopyruvate sulphurtransferase) in different species of the family Cichlidae (Tilapia zillii, Sarotherodon galilaeus and Oreochromis niloticus)

The cyanide metabolising enzymes (rhodanese and mercaptopyruvate sulphurtransferase) were estimated in different tissues of three species of tilapia (Tilapia zilli, Sarotherodon galileus,and Oreochromis niloticus) from two locations (Aiba and Osinmo reservoirs, both in the South-Western region of Nigeria). The enzyme activities were carried out by measuring the amount of thiocyanate produced by the two enzymes...

Author(s): Okonji Raphael Emuebie, Popoola Michael Olaoluwa, Kuku Adenike and Aladesanmi Omolara Titilayo

June 2010

Characterization of antioxidant and antimicrobial compounds of cinnamon and ginger essential oils

Essential oils obtained from the bark of Cinnamomum zeylanicum (cinnamon) and the rhizomes of Zingiber officinale (ginger) were characterized by analytical TLC and GC/MS, and their antimicrobial and antioxidant compounds were detected by TLC-bio-autography assays. Essential oil of cinnamon bark (CEO) was found to be a unique aromatic monoterpene-rich natural source, with trans-cinnamaldehyde...

Author(s): G. S. El-Baroty, H. H Abd El-Baky, R. S. Farag and M. A. Saleh