The objectives of this study were to determine phytochemical screening, phenolic contents, antioxidant and antifungal activities of crude ethanolic extracts from different organs of Bunium incrassatum (Boiss.) Batt. Plant samples were collected from the West of Algeria. Phytochemical tests revealed the presence of phenolic compounds and other several families of chemical constituents. Flavonoids and coumarins were some of the main secondary metabolites recognized in different organs of this plant. The highest total phenolic content was found in leaves extract with a TPC value of 51.22±0.46 mg GAE/g and in which flavonoids were the main elements. Instead, the highest coumarin content was recorded in barks extract. Antioxidant activity was estimated by DPPH scavenging method and compared to the ascorbic acid antioxidant. Leaves extract showed a good scavenging activity with an IC50 value of 0.11±0.57 mg/ml. Antifungal activities were tested against three pathogens fungi mycotoxins’ producers by using mycelial growth, sporulation spores, mycelial biomass assay and mycotoxigenic test. For all antifungal properties tested, leaves extract showed the greatest inhibitory activity against Penicillium expansum MTTC 1344 and Aspergillus flavus MTTC 2799 followed by barks extract.

Keywords: Bunium incrassatum (Boiss.) Batt., Phytochemical screening, Phenolic contents, Antioxidant activity, Antifungal activities, Mycotoxin.