September 2008
Cell passaging rapidly affects expression, secretion and activity of MMP9 as well as mobility of HL60 leukemia cells
The HL60 cell line, derived from acute promyelocytic leukemia cells, can differentiate into neutrophil-like cell following DMSO treatment. Mobility of HL60, or DMSO-differentiated HL60 cells (≠HL60), requires surface expression of adhesion molecules and production of matrix metalloproteinases (MMPs). The aim of this study was to investigate in HL60 and ≠HL60 the effects of cell passaging (over 5 passages after...
September 2008
The effect of the lactoperoxidase (LP) system in combination with container smoking on the microbial quality of goats’ milk was assessed during storage at ambient temperature. The titratable acidity, coliform count (CC) and total bacterial count (TBC) in LP activated milk samples (T2) decreased by 0.13%, 1.6 and 1.33 log10 units, respectively as compared to their respective values in the control (T1) at 7 h of storage. Coliform count and TBC in T2 decreased by 0.33 and 0.20 log10 units, respectively at 7 h of storage as compared to the initial count. When container smoking was combined with the LP system (T4), no acid development was observed in the milk samples. It can be concluded that container smoking combined with the LP system could effectively control microbial growth and extend the shelf life of goats’ milk stored at an ambient temperature by at least 7 h. Key words: Goat milk, lactoperoxidase system, shelf life, smoking.
The effect of the lactoperoxidase (LP) system in combination with container smoking on the microbial quality of goats’ milk was assessed during storage at ambient temperature. The titratable acidity, coliform count (CC) and total bacterial count (TBC) in LP activated milk samples (T2) decreased by 0.13%, 1.6 and 1.33 log10 units, respectively as compared to their respective values in the control (T1) at 7 h...
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