September 2011
Comparative studies on properties of amylases extracted from kilned and unkilned malted sorghum and corn
This study investigated the activities of a-amylase, β-amylase and glucoamylase extracted from kilned and unkilned sorghum and corn. Dry grains of sorghum and corn were obtained, steeped and allowed to undergo malting at room temperature for 48 h. Part of the malted starch was kilned by taken into an oven at 50°C for 24 h while the other part was derootted by hand before further processing. a-amylase,...
September 2011
Bacteriological quality and diarrhoeagenic pathogens on River Njoro and Nakuru Municipal water, Kenya
Waterborne diarrhoeal pathogens are considered a re-emerging threat and are responsible for considerable morbidity and mortality, especially in developing countries. The objective of this study was to determine bacteriological quality, biochemical oxygen demand and presence of diarrhoeagenic pathogens in water samples collected from River Njoro and Nakuru Municipal water. A total of 432 samples were tested, 216 from...
September 2011
Molecular characterization of a gene capable of degrading trichloroethylene, an environmental pollutant
Trichloroethylene (TCE) is a widely used organic solvent and metal degreasing agent, one of the most frequently detected groundwater contaminants and a potential health hazard. Our novel isolate, Bacillus cereus strain 2479 was capable of degrading TCE efficiently. The gene for TCE degradation was PCR amplified from genomic DNA of B. cereus 2479. The amplified gene was cloned into expression vector pUC I8 in the E. coli...
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