August 2011

An effective method for obtaining high quality messenger ribonucleic acid (mRNA) with minimal ribosomal ribonucleic acid (rRNA) contents

Plant transcriptomes are very complex in nature and includes overlapping transcripts, transcribed intergenic regions, and abundant non-coding ribonucleic acids (RNAs). With RNA-Seq approach using next generation sequencing technology, complementary deoxyribonucleic acid (cDNA) library constructed either from Messenger ribonucleic acid (mRNA) or from ribosomal ribonucleic acid (rRNA) minus total RNA can be directly...

Author(s): Vinay Kumar, Kalpesh Katudia, Manoj Kumar Vyas, Navin Srivastava, Kanak Vaidya, Spandan Chaudhary and Surendra Kumar Chikara

August 2011

Genomic DNA extraction protocols from a Moroccan medicinal and aromatic plant Artemisia herba-alba Asso for RAPD-PCR studies

This is the first report on development of protocol for high purity genomic DNA isolation from the Moroccan Artemisia herba-alba schrub leaves and optimization of conditions for RAPD-PCR analysis. Two DNA extraction protocols were specifically developed: QIAgen DNA Kit and protocol developed by Ouenzar et al. (1998). DNA yield and purity were monitored by gel electrophoresis and by determining absorbance at UV...

Author(s): Ourid Ibtissam, Ghanmi Mohamed, EL Ghadraoui Lahsen, Kerdouh Benaissa and Bakkali Yakhlef Salah Eddine

August 2011

Screening of different insect pests of mulberry and other agricultural crops for microsporidian infection

In the present study, different insect pests of mulberry and other agricultural crops were collected from mulberry gardens and agricultural crop fields in and around Mysore, Karnataka, India. The collected insects were screened for microsporidian infection and microsporidian spores were isolated from infected specimens, purified and tentatively designated as NIK- 1Pr, NIK- 1Cc, NIK- 1Cpy, NIK- 1So and NIK- 1Dp. The...

Author(s): Ifat Bashir, S. D. Sharma and Shabir A. Bhat