July 2013
The biocidal and phytochemical properties of leaf extract of Cassia occidentalis linn.
The antibacterial potentials of Cassia occidentalis leaf extracts were investigated against eleven Gram-positive and four Gram-negative bacterial isolates. The n-hexane and dichloromethane fractions of the plant extract exhibited appreciable antibacterial action against nine out of the fifteen bacterial isolates tested at a concentration of 20 mg/ml. The zones of inhibition exhibited by...
July 2013
Antibacterial activity of methanol and ethanol leaf extracts of Antidesma venosum and Lannea barteri
Methanol and ethanol cold percolation leaf extracts of Antidesma venosum and Lanneabarteri were screened for their in vitro antibacterial activity against Staphylococcus aureus, Escherichia coli, Proteus vulgaris, Salmonella typhi, Streptococcus lactis and Shigella sp. using the agar well diffusion method. Methanol extracts gave higher...
July 2013
Staphylococcus aureus protein A gene typing by PCR-RFLP
Staphylococcus aureus protein A coding gene (spa) is a proper gene for typing of S. aureus and used to trace in the epidemiological studies. The aim of this study was to explore the typing of S. aureus by polymerase chain reaction restriction fragment-length polymorphism (PCR-RFLP) spa gene. Hae II restriction enzyme (Fermentase Co, Germany) method was used to digest PCR...
July 2013
Variation of virulence profiles in some Staphylococcus aureus and Pseudomonas aeruginosa stains isolated from different clinical patients
Considering the great diversity and prevalence of opportunistic pathogens as well as the medical consequences arising from increasingly frequent cases of infections with resistant pathogens, the aim of this study was to analyze and compare the virulence profiles of some recent isolates of the most frequently encountered human pathogens,Staphylococcus aureus and Pseudomonas aeruginosa....
July 2013
Experimental studies on synergism between meropenem and sulbactam
Meropenem had been successfully used independently against various types of infections when it was first discovered, while sulbactum sodium being much less potent had been given to humans more frequently in combination with ampicillin. Meropenem is now less frequently applied alone in infections caused by virulent multidrug resistant Gram negative organisms. Further, potentiation of action of meropenem is...
July 2013
Antiproliferative and antimicrobial activities of kombucha tea
The present study was conducted to evaluate the antiproliferative and antimicrobial activities of kombucha tea. We investigated in vitro the efficacy of kombucha tea for its antiproliferative effects by its potential cytotoxic activity using the MTT colorimetric method against two human cancer cell lines (A549, lung cell carcinoma and Hep-2, epidermoid carcinoma). Treatment of human...
July 2013
Effect of beneficial bacteria on larval culture of Pacific whiteleg shrimp, Litopenaeus vannamei
Beneficial microorganisms isolated from the gut of adult shrimp, Litopenaeus vannameiand adult Cortez oyster, Crassostrea corteziensis was tested to determine larval survival of whiteleg shrimp L. vannamei. The isolates were characterized through tests for hemolytic and antagonism to select potential beneficial strains. Strains YC5-8, Y02-1, YC5-1 and YPD10-4 were selected from in...
July 2013
Seroprevalence of Toxoplasma gondii infection in dogs in Jinzhou City, Northeast China
To investigate the prevalence of dogs exposed to Toxoplasma gondii in Jinzhou City, Liaoning Province, northeast China, 304 dogs were screened for specific antibodies to T. gondii by enzyme-linked immunosorbent assay (ELISA) using a commercially-marketed kit. The overall prevalence of T. gondii IgG antibodies were found in 82 of 304 (26.97%) dogs with regional variations, but...
July 2013
Carbon sources and pH effect on pectinolytic activity production by Ascochyta rabiei isolated from chickpea (Cicer arietinum L.) in West Algeria
In Algeria, Ascochyta blight is a major limiting factor in chickpea production. We did not record any difference between the isolates while basing ourselves on the morphological and cultural characters, but the pectinolytic activity could differentiate the ten isolates ofAscochyta rabiei. Activity of pectin methyl esterase was baseless in the presence of glucose, but variable in the presence of the...
July 2013
Quantitative determination of vitamin B6 in dietary foods for special medical purposes by microbiological assay method
“Dietary food for special medical purposes” is defined as the foodstuffs that are specifically produced or formulated and consumed under medical supervision with the aim of establishing diets of patients. These foods labeled and marketed are generally liquid or powder products formulated to meet specific needs. The objective of this research was to quantitative determination vitamin B6 (pyridoxine)...
July 2013
Optimization of medium composition for α-galactosidase production by Antarctic bacterial isolate, Bacillus sp. LX-1 using response surface methodology
The optimization of medium composition for α-galactosidase production by an Antarctic bacterial isolate, Bacillus sp. LX-1 was investigated in flask fermentation. The Central composite design (CCD) with 3 variables (galactose, peptone and MnSO4) was employed to derive statistical model (Response surface methodology: RSM) for optimizing the medium...
July 2013
Identification and antimicrobial susceptibility of Staphylococcus aureus isolated from milk samples of dairy cows and nasal swabs of farm workers in selected dairy farms around Addis Ababa, Ethiopia
Staphylococcus aureus is a major problem of public health which causes a number of human and animal diseases. In order to isolate and identify S. aureus from milk of dairy cows and nasal swabs of farm workers of different farm settings, a cross sectional study was conducted on a total of 260 lactating dairy cows milk and 68 nasal swabs of farm workers in selected dairy farms...
July 2013
Erythritol biosynthesis by Yarrowia lipolytica yeast under various culture conditions
Erythritol is a natural compound of great interest in food production because of its very low caloric value, lack of off-taste and lack of side-effects from the gastric system even when consumed with in excess. In the present study, the effect of agitation rates from 500 to 1100 rev/min on biomass and erythritol production on glycerol media by an acetate-negative mutant of Yarrowia...
July 2013
Isolation of Lecythophora sp. YP363, a secretor of various thermostable plant cell wall-degrading enzymes with high activity
A fungal strain that produces high activities of various plant cell wall degrading enzymes such as CMCase, filter paperase, β-glucosidase, avicelase, xylanase and pectinase with high activities was isolated from soil surrounding rotten wood. The strain was identified asLecythophora sp. YP363 based on its ITS, 18S and 26S sequences. The specific activities of the various plant cell wall-degrading enzymes...
July 2013
Antimicrobial and antioxidant activities of Pimenta malagueta (Capsicum frutescens)
Pimenta malagueta (Capsicum frutescens), as it is known in Brazil, is one of the most commonly used pepper species in cooking and in Brazilian folk medicine. In this work, the total phenolic compounds and the capsaicin, dihydrocapsaicin, and chrysoeriol contents of C. frutescens were analysed. Additionally, the antioxidant and antimicrobial activities were determined. The contents of capsaicin,...
July 2013
In vitro solubilization of inorganic phosphates by phosphate solubilizing microorganisms
Bacterial strains isolated from green house soils were incubated in vitro for seven days to assess their phosphate solubilizing capacity, solubilization index, organic acid production and acid and alkaline phosphatase activity. All the tested isolates solubilized inorganic phosphates in varying degrees in NBRIP liquid culture medium. Acidification by means of producing organic acids may be the key...
July 2013
Recombination study using Radish leaf curl virus isolates
Begomovirus isolates, exhibiting identity of 89 and 93% with Radish leaf curl virus(RaLCV), were detected from the leaf curl disease affected okra (Abelmoschus esculentus) plants in Bihar, India. The detected isolates are supposed to be a new strain of RaLCV and therefore are referred to here as RaLCV-Hajipur1 and RaLCV-Hajipur2. Alphasatellite was identified as Cotton leaf curl Burewala alphasatellite...
July 2013
Growth inhibitory potential of polyene type metabolite producing Streptomyces speibonae ERI-01
A Gram positive, filamentous, spore forming antagonistic Streptomyces speibonae ERI-01derived from Western Ghats of Tamil Nadu, India, was studied for its antagonistic activities. Preliminary screening revealed that 38% of Streptomyces strains showed activity against Bacillus subtilis (34%), Staphylococcus aureus (32.4%), Staphylococcus...
July 2013
Development of polymorphic microsatellite markers from Verticillium dahliae, a pathogenic fungus of cotton
Thirteen polymorphic microsatellite loci originating from the genome sequence were developed for cotton pathogenic fungus, Verticillium dahliae. The simple sequence repeats (SSR) markers were used to characterize the genotypic diversity of 30 isolates ofV. dahliae. Thirteen loci displayed the number of alleles per locus (Na) ranged from 2 to 5 with an average of 3 and effective number of alleles...
July 2013
Mutations in the quinolone resistance determining region in isogenic mutant Acinetobacter baumannii strains
The aim of the study is to explore the change or mutation of DNA gyrase (gyrA) and topoisomerase IV (parC) in Quinolone Resistance Determining Region (QRDR) inAcinetobacter baumannii isogenic mutant strains which were insensitivity or resistant to fluoroquinolones (FQs). A total of 11 strains were studied, one is isolated from clinical sample as a wide-type parent strain and the others isogenic mutant...
July 2013
Antifungal metabolites from the fermentation broth of plant endophytic fungus Pestalotiopsis photiniae
Pestalotiopsis genus, one of the most potential resources for biological active compounds, can produce most interesting compounds with broad activity spectrum. In the course of our research on bioactive metabolites of the genus Pestalotiopsis in China, the present study was undertaken to investigate the bioactive chemical constituents of the culture broth of Pestalotiopsis...
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