July 2013
Bacterial biodegradation and decolourization of toxic textile azo dyes
Textile industry is considered as one of the largest generators of toxic chemical waste water in India. Dyes released by the textile industries pose a threat to environmental safety. Recently, dye decolourization through biological means has gained momentum as these are cheap and can be applied to wide range of dyes. This review paper focuses on the bacterial biodegradation of toxic textile dyes. Along with...
July 2013
Rift Valley fever outbreaks: Possible implication of Hyalomma truncatum (Acari: Ixodidae)
Rift Valley fever (RVF) is a viral zoonosis that primarily affects livestock. However, the virus can also infect humans and may even cause human fatalities. Although the vast majority of human infections result from direct or indirect contact with the blood or organs of infected animals, human infections have also resulted from the bites of infected Aedesmosquitoes. Due to the increasing...
July 2013
Microorganisms isolated from surgical wounds infection and treatment with different natural products and antibiotics
Surgical site infections (SSIs) are common nosocomial infections in surgical patients resulting in significant increases in postoperative morbidity and mortality. This study aimed to isolate and identify bacteria that cause SSIs in Medical Research Institute, Alexandria University, Egypt, and compare their sensitivities to selected group of antibiotics and natural products. Isolates from 20 patients with SSIs...
July 2013
Evaluation of anti-Candida activity and cytoxicity of Jatropha dioica Cerv. extracts
The current study evaluates the antifungal activity and cytotoxicity of aqueous and 70% ethanol (EtOH) extracts of the leaves and roots of Jatropha dioica Cerv.. The extracts were obtained from the suspension of 18 g of the aerial (leaves) and root parts of J. dioicaCerv. in 200 ml of 70% EtOH and water for 24 h. Serial dilutions ranging from 14.06 to 450 µg/ml were made from a...
July 2013
Urinary tract infections caused by Streptococcus uberis: A pathogen of bovine mastitis-report of seven cases
Environmental Streptococci, predominantly Streptococcus uberis are the leading cause of bovine mastitis. However, S. uberis is rarely associated with human infections, although, a limited number of human infections by S. uberis have been reported. In this study, seven cases of urinary tract infections (UTIs), out of 148 culture positive urinary infections...
July 2013
A study on the sterility, safety, potency and purity of hydropericardium hepatitis syndrome (Angara disease) vaccines
The present study was conducted in order to determine the quality of commercially available Angara disease vaccines. For the experiment, 200 broiler chicks of one day old were reared at National Veterinary Laboratories, Islamabad in isolation units. Chicks were divided into three groups that is, 1, 2, 3 and having 25, 25 and 150 chicks, respectively. Group 1 was used to determine LD50 of the virus in...
July 2013
Optimisation of reverse transcription loop-mediated isothermal amplification assay for the rapid detection of pandemic (H1N1) 2009 virus
Conventional reverse transcriptase polymerase chain reaction (RT-PCR) and optimized of a closed tube reverse-transcription loop-mediated isothermal amplification (RT-LAMP) were used for detection of pandemic (H1N1) 2009 virus and the optimized of a closed tube RT-LAMP methods were compared with the conventional RT-PCR with respect to specificity and sensitivity. In this study, optimized RT-LAMP detected 2...
July 2013
A fuzzy logic based prediction method for determining Brucellosis seroprevalence
Brucellosis is an important zoonotic disease worldwide and it affects the health of animals, human and the livestock incomes negatively. Although it is an animal illness, it infects human by contact with infected animals or consuming contaminated milk or milk products. Modeling studies established to obtain seroprevalance of a disease is important in order to determine statistics and measuring strategies...
July 2013
Random amplified polymorphic DNA (RAPD) analysis of microbial community diversity in soil affected by industrial pollutants: Reference to Mandideep industrial area
The present study was aimed to investigate microbial diversity of industrially contaminated and uncontaminated agriculture field soil using random amplified polymorphic DNA (RAPD) analysis. Soil samples were collected from highly contaminated (industrial pollution) and uncontaminated agriculture fields away from polluted area. RAPD analysis was carried out using four ten-mer primers namely RBa-3, 4, 5 and 6 to find out...
July 2013
Heavy metal and sulfur concentrations and mycorrhizal colonizing status of plants from abandoned lead/zinc mine land in Gejiu, Southwest China
A field survey of heavy metals and sulfur concentrations and mycorrhizal status of plants grown in abandoned lead/zinc mine land was conducted in Gejiu City, Yunnan Province, Southwest China. Low or no root arbuscular mycorrhizal fungi (AMF) infection was detected in most of the plants for 21 species of 13 families collected from the abandoned Pb/Zn mine land. The maximum concentrations of Pb, Zn, Cd and...
July 2013
Comparative behavior of terminal heat tolerant (WH 730) and intolerant (Raj 4014) hexaploid wheat genotypes at germination and growth at early stage under varying temperature regimes
Wheat (Triticum aestivum L.) is a winter season crop and India holds second rank in production globally. Several reports have supported the facts that heat and cold stress adversely affect the germination, crop growth and development. Terminal heat stress is a major problem for wheat production which severely affects the grain yield. Two wheat genotype viz. WH 730 (heat tolerant) and Raj 4014...
July 2013
Varietal response of African yam bean, Sphenostylis stenocarpa (Hochst Ex. A. Rich) Harms to infection with Meloidogyne incognita (Kofoid & White) Chitwood under field conditions
A pot study demonstrated that four and eight African yam bean (Sphenostylis stenocarpa) accessions were tolerant and susceptible to the root-knot nematode (Meloidogyne incognita), respectively. A study was, therefore, conducted to confirm the pot results under field conditions. Experimental design was randomized complete block design with 3 replications. A total of 750 nematode juveniles per 500 g soil sample...
July 2013
Induction of systemic resistance in green gram against leaf blight caused by Macrophomina phaseolina (Tassi.) Goid
Studies were conducted to develop the sustainable plant protection strategies against leaf blight of green gram incited by Macrophomina phaseolina by using plant extracts and fungicides under in vitro and in vivo conditions. In vitro studies indicated that plant leaf extracts of Adenocalymma alliaceum (77.20%) and Allium sp. (74.71%) demonstrated the...
July 2013
Culture-dependent and culture-independent techniques to identify lactic acid bacteria in fermented products
Ten commercially available probiotic products were collected from the local market of Lahore, Pakistan; including dairy, freeze dried products and fruit drinks. These products were examined by using culture dependent methods, SDS-PAGE as well as by visualization of denaturing gradient gel electrophoresis (DGGE) band profiles under UV light. Identical results of the DGGE and culture-dependent analysis of...
July 2013
Physiological and molecular identification of polyhydroxybutyrates (PHB) producing micro-organisms isolated from root nodules of leguminous plants
Polyhydroxybutyrates (PHBs) are synthesized and deposited as cytoplasmic inclusions in various bacteria like Rhizobium sp., Rhodobacter sphaeroides, etc. This work was focused on isolating a PHB-producing bacterium capable of growing in conventional media used for industrial biopolymer production, its physiological and molecular identification and characterization. Thus, a bacterial isolation process was...
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