August 2013
Inorganic phosphate solubilization by phosphate solubilizing fungi isolated from acidic soils
The experiment was conducted to evaluate the native phosphate solubilizing fungi on inorganic phosphate solubilization in the acidic soils of Jharkhand, India. Mineral phosphate solubilizing activities of three fungal isolates were tested in tricalcium phosphate and ferric phosphate medium by analyzing the possible phosphorus release and phosphatase activity from 5th, 10th and 15th day of...
August 2013
A new molecular diagnosis method combined single primer isothermal amplification with rapid isothermal detection assay in detection of group B Streptococcus
Herein, a new molecular diagnosis assay for rapid detection of bacterial DNA was established, which combined single primer isothermal amplification (SPIA) with rapid isothermal detection assay (RIDA). Using this method, we could rapidly identify Group BStreptococcus DNA with high sensitivity and specificity, the detection limit of the new method was 102copy/mL. The whole assay was processed in one tube...
August 2013
Presence of diarrheagenic Escherichia coli pathotypes, Salmonella and indicator bacteria on fresh-cut jicama [Pachyrhizus erosus (L.) Urban] from public markets in Mexico
Fecal coliforms (FC), Escherichia coli, diarrheagenic E. coli pathotypes (enterotoxigenic, enteropathogenic, enteroinvasive, enteroaggregative and non-O157 Shiga toxin-producing) and Salmonella frequencies were determined for fresh-cut jicama [Pachyrhizus erosus (L.) Urban] root. One hundred fresh-cut jicama samples were collected from public markets in Pachuca, Mexico....
August 2013
Infrared spectroscopy: Methods for investigating cellular components of phytopathogenic fungi response to temperature stress
Fourier-transform infrared (FTIR) spectroscopy is a sensitive method for the investigation of fungi biochemical composition changes in cells. FTIR spectroscopy can be used to study fungal cell biology by fingerprinting varieties of carbohydrates, proteins, and lipids at about 6 µm spatial resolution. FTIR can distinguish fungal changes during stressed...
August 2013
Cholesterol reduction by Lactococcus lactis KF147
Lactococcus lactis from yoghurt sample was confirmed based on alignment of 16S rRNA gene sequence in gene bank. The degree of acid and bile tolerance of strain L. lactissubsp. lactis KF147 was evaluated. The L. lactis KF147 was able to maintain viability for 2 h at pH 2 and growth in a medium with 0.4% (w/v) of bile acids. The maximum cholesterol reduction was 66.8% from the mMRS...
August 2013
Antibiotic potential of Lunularia cruciata (L.) Dum ex. Lindb (bryophyta) of Kumaon Himalaya
Antibiotic activity of Lunularia cruciata was tested against five pathogenic bacteria (Agrobacterium tumefaciens, Xanthomonas phoseoli, Escherichia coli, Bacillus subtilisand Erwinia chrysanthemi) and three pathogenic fungi (Alternaria alternata, Sclerotinia sclerotiorum and Pyricularia oryzae). The plant extract was prepared in various solvents (ethanol, methanol, acetone, chloroform...
August 2013
Microbiological and physico-chemical changes during fermentation of maize for masa production
Samples of traditionally fermenting maize meal popularly called masa were collected from local producers of masa in Ibadan, Oyo State, Nigeria and were analysed for pH, total titratable acidity (TTA), microbiological changes and proximate analysis. The sensory properties (colour, texture, taste, sourness, appearance and general acceptability) were also carried out on a nine point...
August 2013
Studies on the effects of microbial fermentation on bush mango (Irvingia gabonensis) seed cotyledons
Fermentation is known to improve the nutritional value of food samples. This study was conducted to assess the influence of fermentation on bush mango in order to reduce environmental pollution. Bush mango seed cotyledons were naturally fermented for five days at room temperature. The bacteria and fungi responsible for the fermentation were isolated and characterised using standard microbiological techniques. The...
August 2013
Multiplex polymerase chain reaction (PCR) for the detection of diarrheagenic Escherichia coli and Shigella directly from stool
Shigella and diarrheagenic Escherichia coli (DEC) are the most common causes of diarrheal diseases in developing countries. A multiplex polymerase chain reaction (mPCR) was developed for identification of DEC infections caused by shiga toxin producing E. coli (STEC), enteropathogenic E. coli (EPEC), enterotoxigenic E. coli(ETEC), enteroinvasive E. coli (EIEC)...
August 2013
Antibiotic resistant profiles of food (fresh raw milk) and environmental (abattoir effluents) isolates of Listeria monocytogenes from the six zones of Nigeria
The prevalence of Listeria monocytogenes in fresh raw milk and abattoir effluents in the six zones of Nigeria was determined. Antibiotic resistant profile of the isolates was examined using the Bauer- Kirby disc diffusion assay. A total of 626 food and environmental samples were cultured on selective media out of which 54 (8.6%) were positive for L. monocytogenes. Chloramphenicol was the most...
August 2013
Isolation and characterisation of aflatoxigenic Aspergillus species from maize and soil samples from selected counties of Kenya
Aflatoxin contamination of maize put the health and well-being of Kenyan people at risk, primarily children. Aflatoxigenic fungi can infect grains from pre-harvest stages in the field to post-harvest stages in the stores. The aim of this study was to isolate and characterize aflatoxigenic Aspergillus spp. from maize and soil samples from selected counties of Kenya: Makueni, Nyeri, Bungoma,...
August 2013
Algicidal activity of Ulva pertusa and Ulva prolifera on Prorocentrum donghaiense under laboratory conditions
Allelopathic effects of fresh tissue and dry powder of two bloom-forming green macroalgae Ulva pertusa and Ulva prolifera on microalga Prorocentrum donghaiensewere evaluated at different concentrations. The filtrates of macroalga culture medium were investigated for their inhibitory effect on P. donghaiense using initial or semi-continuous filtrate addition. Preliminary...
August 2013
Bioefficacy of plant extracts and biocontrol agents against Alternaria solani
Three bioagents viz., Trichoderma harzianum Rifai, Trichoderma viride Pers. andTrichoderma virens (Miller et al) von Arx and five plant extracts viz., Artimesia absinthiumL., Datura stramonium L., Urtica dioica L., Juglans regia L. and Mentha arvensis L. were evaluated in vitro against Alternaria solani (Ellis and Martin) Jones...
August 2013
Solubilization of tricalcium phosphate by temperature and salt tolerant Serratia marcescens NBRI1213 isolated from alkaline soils
A number of stress tolerant plant growth promoting phosphate solubilizing bacteria were isolated from soils collected from different geo-climatic zones of India. Maximum numbers of these bacteria were found in Aurawan soil, followed by National Botanical Garden, Mukhateshwar and Nainital. Among these bacteria, strain NBRI1213 showed maximum phosphate solubilization activity (984 µg/mL) and was...
August 2013
Molecular investigation of antibiotic resistance genes in methicillin resistant Staphylococcus aureus isolated from nasal cavity in pediatric service
Methicillin-resistant staphylococci may also be resistant to some other antibiotics as well as β-lactams. Co-existence of resistance to methicillin and macrolides was genetically investigated in Staphylococcus aureus. A total of 59 S. aureus strains isolated from nasal cavity of patients hospitalized in the pediatric service were investigated for their susceptibility to...
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