March 2012

Growth arrest induction of 3T3-L1 preadipocytes by serum starvation and their differentiation by the hormonal adipogenic cocktail

The current methods for differentiation of 3T3-L1 preadipocytes start with growth arrest by contact inhibition. However, on day 6, 3T3-L1 preadipocytes reliably detach and differentiation could no longer proceed. In the present study, we used serum starvation to induce growth arrest in 3T3-L1 preadipocytes and investigated their differentiation by the modified hormonal adipogenic cocktail.  About 85% of 3T3-L1...

Author(s): Zhenhui Cao#, Hui Yang#, Lingfu Kong, Dahai Gu, Zhanlong He, Zhiqiang Xu, Junjing Jia, Changrong Ge and Qiuye Lin

March 2012

Evaluation of mice, sheep and human IgG and IgE antibody responses against the mice Crude Hydatid Cyst Fluid (HCF) antigens

Hydatidosis is a dangerous parasitic disease which commonly occurs in humans and animals. It annually causes many economic and health problems throughout the world. The existing diagnostic kit uses sheep cystic fluid antigen as an available antigen to evaluate the human IgG antibody. The current study was designed to evaluate an alternative antigen which is the mice HCF antigen for such diagnosing kit. For this purpose...

Author(s): Afra Khosravi, Morteza Shamsi, Noorkhoda Sadeghifard and Ghafourian Sobhan