April 2011

Effect of genotype, explant source and medium on in vitro regeneration of tomato

For the present investigations, five cultivars including PKM-1, Moneymaker, Microtom, Micro-MsK and White Cherry of tomato (Lycopersicon esculentum Mill.) were selected. The explants cotyledon, hypocotyl and leaf of all the cultivars of tomato were cultured on MS medium fortified with different concentrations of Benzyl amino purine (BAP) (1.0 to 3.0 mg/L)and 0.1...

Author(s): Praveen Mamidala, and Rama Swamy Nanna

April 2011

Plasmid-mediated transformation of Escherichia coli having a dependence on the heat-shock protein GroEL

When the cells of Escherichia coli mutant in heat-shock regulator protein sigma-32 were transformed with plasmid pUC19 DNA, the transformation efficiency (TRE) of the mutant strain was about 10% of the wild type (wt) strain, indicating the role of heat-shock protein(s) in the transformation process. In order to search for the protein(s), when three different E. coli mutants in three important...

Author(s): Pulakesh Aich, Arijit Kumar Chatterjee, Monobesh Patra and Tarakdas Basu