May 2009

The Hsp40-Hsp70 chaperone machinery of Plasmodium falciparum

  Plasmodium falciparum is the protozoan parasite responsible for the most virulent form of malaria. The majority of the asexual stages of its life cycle occur in the human erythrocyte. Since the infected erythrocytes undergo dramatic structural and functional changes upon parasite infection, malaria research has been focusing on investigating the proteins potentially involved in host cell...

Author(s): Eva-Rachele Pesce and Gregory L. Blatch

May 2009

The use of circular dichroism spectroscopy to study protein folding, form and function

Circular Dichroism (CD) is a spectroscopic technique widely used for the evaluation of the conformation and stability of proteins in several environmental conditions like temperature, ionic strength, and presence of solutes or small molecules. Circular Dichroism spectroscopy is non-destructive, relatively easy to operate, requires small amount of sample and few data collection. Additionally, data analyses are fast....

Author(s): Daniel H. A. Corrêa,  and Carlos H. I. Ramos