African Journal of
Biochemistry Research

  • Abbreviation: Afr. J. Biochem. Res.
  • Language: English
  • ISSN: 1996-0778
  • DOI: 10.5897/AJBR
  • Start Year: 2007
  • Published Articles: 425

Full Length Research Paper

Thermostable, alkaline tolerant lipase from Bacillus licheniformis using peanut oil cake as a substrate

N. Annamalai*, S. Elayaraja, S. Vijayalakshmi and T. Balasubramanian
Marine Sciences, CAS in Marine Biology, Annamalai University, Parangipettai 608502, Tamil Nadu, India.
Email: [email protected]

  •  Accepted: 10 February 2011
  •  Published: 30 June 2011

Abstract

Thermostable alkaline tolerant lipase producing Bacillus licheniformis isolated from marine environment. Peanut oil cake was used as substrate for enzyme production and purified to 3.6 fold with a specific activity of 148.4 U/mg of protein with molecular weight of 35 kDa. The enzyme was 100% stable at 60°C, it retains more than 80% activity after 1 h incubation at 70°C and pH 9-11.5. Metals like Ca2+, Ba2+ and Mg2+ enhanced whereas Ni2+ and Fe3+ inhibited the enzyme activity. No effect on enzyme activity found when treated with Tween 80 and sodium deoxycholate, but completely inhibited with SDS.

 

Key words: Bacillus licheniformis, lipase, peanut oil cake, enzyme activity, thermostable, metals.

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This article is published under the terms of the Creative Commons Attribution License 4.0

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