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Expression of MUC2 and MUC5B in ethmoid sinus mucosa of
patients with chronic rhinosinusitis
Huang Xue-Kun*, Li Yuan, Ye Jin, Li Peng and Liu Hong
Department of Otolaryngology-Head and Neck Surgery, the
Third Affiliated Hospital of Sun Yat-Sun University,
Guangzhou 510630, China.
*Corresponding author: E-mail:
huang024326@qq.com.
Accepted 3 June, 2010 |
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This study investigated the expression of MUC2 and MUC5B in
the ethmoid sinus mucosa of patients with chronic
rhinosinusitis (CRS). Immunohistochemistry and fluorescence
quantitative reverse transcription polymerase chain reaction
(RT-PCR) were performed to detect the expression of MUC2 and
MUC5B in the ethmoid sinus mucosa of 32 patients with CRS
and 7 control individuals. Immunohistochemistry confirmed
the expression of MUC2 and MUC5B in the ethmoid sinus mucosa
of both patients and control subjects, and the expression of
both proteins in CRS patients was significantly higher than
that in control individuals (p < 0.01). The mRNA expression
of MUC2 and MUC5B was 10.55 ± 3.91 × 104 and
18.80 ± 15.68 × 105 copies/μg in CRS patients,
and 1.36 ± 0.46 × 104 copies/μg and 1.49 ± 0.46 ×
105 copies/μg in control individuals,
respectively, with significant differences between the two
groups(p < 0.01). Statistical analysis demonstrated the
markedly increased expression of MUC2 and MUC5B in the
ethmoid sinus mucosa of CRS patients, when compared with
control individuals. The expression MUC2 and MUC5B in the
ethmoid sinus mucosa of CRS patients was up-regulated, and
the up-regulated expression may result in mucus
hypersecretion and compromised mucociliary clearance in CRS
patients.
Key words:
chronic rhinosinusitis, mucin, immunohistochemistry,
fluorescence quantitative polymerase chain reaction. |
