|
Response of Culex
quinquefasciatus to deltamethrin in Lahore district
Hafiz Muhammad Tahir1*,
Abida Butt1 and Shafaat Yar Khan2
1Department
of Zoology, University of the Punjab, Lahore, Pakistan.
2Department
of Biological Sciences, University of Sarghoda, Pakistan.
*Corresponding author. E-mail:
hafiztahirpk1@yahoo.com.
Accepted
8 October, 2009. |
|
In the
present study, both male and females of
Culex
quinquefasciatus
were
sampled from five different localities of Lahore District of
Punjab, Pakistan. (that is, Jaman, Mohlanwal, Noorpur Bhatta
A1, Yohanaabad–2 and GCU Lahore). Bioassays against 5%
deltamethrin were performed to evaluate the level of
resistance in Culex quinquefasciatus against deltamethrin. For the adult bioassays standard protocol of WHO
was adopted. Results of the bioassays showed that
individuals of all populations were susceptible to 5% of
deltamethrin. Male specimens from all populations showed
significantly higher mortality as compared to females.
Biochemical tests were performed to measure the esterase
activity, both in males and females of C.
quinquefasciatus. Significant variation was observed in
the level of esterases in C. quinquefasciatus
populations, collected from five different localities. Alpha
naphthyl acetate was hydrolyzed faster than beta naphthyl
acetate by mosquito homogenates of Yohanabad-2, GC
University Lahore and Noorpur Bhatta A1 while mosquito
homogenates of Mohlanwal and Jaman population hydrolyzed the
beta naphthyl acetate faster than alpha naphthyl acetate.
Only one band was observed in all populations for esterases
A. For esterases B band pattern was similar in Yohanabad-2
and GC University. In these two populations there were three
bands (B1, B2 & B3). In the population of Noorpur Bhatta A1,
Mohlanwal and Jaman band B2 was absent. There was no
resistance detected in C. quinquefasciatus from Jaman,
Mohlanwal populations after 1 h exposure to 5% deltamethrin
while population of GC University, Yohanabad and Noorpur
Bhatta A1 showed a high level of tolerance to deltamethrin.
Key words:
Esterases, Culex quinquefasciatus, resistance,
pyrethroids. |
