This study has evaluated the antibacterial effect of Jojoba oil (J.O) in vivo against Staphylococcus aureus. One hundred mature male Sprague Dawley rats were divided into four groups, including the control groups and the groups treated with J.O. as part of the diet mixture (2.5% of total body weight). Our findings suggest a significant increase in body weight gain (94.0 ±5.404 g for J.O treated rats in comparison to 72.0 ±3.077 g for untreated rats), final body weight (232.0 ±7.277 g for J.O treated rats in comparison to 220.8 ±9.71 g for untreated rats), and internal organs (0.31±0.03 g, 0.802±0.017 g and 3.88±0.160 g for spleen, kidney and liver respectively in J.O treated rats in comparison to 0.268±0.052 g, 0.786±0.012 g and 3.708±0.125 g for spleen, kidney and liver respectively in untreated rats). On the other hand, rats infected with S. aureus and fed by ration mixed with J.O. showed insignificant increase in all studied parameters. Average body weight gain was 74.8±3.673 g and internal organs weight were 0.826±0.028, 0.358±0.055 and 4.124±0.275 g for kidney, spleen and liver respectively. The positive control group showed acute septicemia. Blood samples were taken from diverse groups and were used for bacteriological tests and radial immune diffusion tests. Results have revealed that S. aureus was in pure form and identified biochemically.

Keywords: Jojoba oil, antimicrobial activity, in vivo, Staphylococcus aureus.