Journal of Pharmacognosy and Phytotherapy
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Article Number - C64A0525196


Vol.4(6), pp. 75 - 85 , October 2012
DOI: 10.5897/JPP12.008
ISSN: 2141-2502



Full Length Research Paper

Anti-diabetic activity of Ocimum sanctum L. roots and isolation of new phytoconstituents using two-dimensional nuclear magnetic resonance spectroscopy


M. Zaffer Ahmad*, Mohd. Ali and Showkat R. Mir




Phytochemical Research Laboratory, Department of Pharmacognosy and Phytochemistry, Faculty of Pharmacy, Jamia Hamdard, New Delhi-110062, India.


Email: muzizaffer@gmail.com






 Accepted: 23 August 2012  Published: 31 October 2012

Copyright © 2012 Author(s) retain the copyright of this article.
This article is published under the terms of the Creative Commons Attribution License 4.0


In view of alleged antidiabetic potential, effect of methanolic extract of Ocimum sanctumL. (Lamiaceae) roots on fasting blood sugar levels and serum biochemical analysis in streptozotocin-induced diabetic rats were investigated. The resulted extract had shown significant protection and lowered the blood glucose levels to normal in glucose tolerance test. In long term treatment of streptozotocin-induced diabetic rats, the degree of protection was determined by measuring blood glucose, triglycerides, total cholesterol and serum insulin levels. Phytochemical investigation of the roots resulted in the isolation of three new active constituents characterized as urs-12-en-3β,6β,20β-triol-28-oic acid (2), 1´´-menthyl-2-glucopyranosyloxybenzoate (4) and n-decanoyl-β-D-glucopyranosyl-(2a→1b)-β-D-glucopyranosyl-(2b→1c)-β-D-glucopyranosyl-(2c→1d)-β-D-glucopyranosyl-2d-2΄-hydroxybenzoate (5), along with known compounds of ursolic acid (1) and palmityl glucoside (3). The structures of the isolated compounds were established by proton nuclear magnetic resonance (1H-NMR), carbon-13 NMR (13C NMR), fast atom bombardment (FAB) mass, 1H-1H correlation spectroscopy (COSY) and heteronuclear multiple-bond correlation (HMBC) spectral techniques.

 

Key words: Ocimum sanctum L., roots, antidiabetic activity, phytoconstituents.


APA (2012). Anti-diabetic activity of Ocimum sanctum L. roots and isolation of new phytoconstituents using two-dimensional nuclear magnetic resonance spectroscopy. Journal of Pharmacognosy and Phytotherapy, 4(6), 75 - 85.
Chicago M. Zaffer Ahmad, Mohd. Ali and Showkat R. Mir. "Anti-diabetic activity of Ocimum sanctum L. roots and isolation of new phytoconstituents using two-dimensional nuclear magnetic resonance spectroscopy." Journal of Pharmacognosy and Phytotherapy 4, no. 6 (2012): 75 - 85.
MLA M. Zaffer Ahmad, Mohd. Ali and Showkat R. Mir. "Anti-diabetic activity of Ocimum sanctum L. roots and isolation of new phytoconstituents using two-dimensional nuclear magnetic resonance spectroscopy." Journal of Pharmacognosy and Phytotherapy 4.6 (2012): 75 - 85.
   
DOI 10.5897/JPP12.008
URL http://www.academicjournals.org/journal/JPP/article-abstract/C64A0525196

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