Journal of
Medicinal Plants Research

  • Abbreviation: J. Med. Plants Res.
  • Language: English
  • ISSN: 1996-0875
  • DOI: 10.5897/JMPR
  • Start Year: 2007
  • Published Articles: 3548

Full Length Research Paper

Evaluation of Procavia capensis hyraceum used in traditional medicine for modulation of mutagen-induced genotoxicity

Asita Okorie Asita
  • Asita Okorie Asita
  • Department of Biology, National University of Lesotho, P. O. Roma 180, Maseru 100, Lesotho, Southern Africa.
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Thabang Rants’o
  • Thabang Rants’o
  • Department of Biology, National University of Lesotho, P. O. Roma 180, Maseru 100, Lesotho, Southern Africa.
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Sibusisiwe Magama
  • Sibusisiwe Magama
  • Department of Biology, National University of Lesotho, P. O. Roma 180, Maseru 100, Lesotho, Southern Africa.
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Matsepo Taole
  • Matsepo Taole
  • Department of Biology, National University of Lesotho, P. O. Roma 180, Maseru 100, Lesotho, Southern Africa.
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  •  Received: 19 April 2018
  •  Accepted: 22 May 2018
  •  Published: 10 June 2018

Abstract

Hyraceum (HM) is used in traditional medicine in Southern Africa. Three concentrations of HM (mg/ml in distilled water) (0.0156, 0.03125 and 0.0625) were assessed for cytotoxicity (CT), genotoxicity (GT) and modulation of cyclophosphamide (CP)- and ethyl methanesulfonate (EMS)-induced GT using the Allium cepa assay following 24 h treatment.  CP (1.00 mg/ml) and EMS (0.0375 mg/ml) were not cytotoxic but genotoxic. HM (0.03125, 0.0625 mg/ml) and its mixtures with CP or EMS induced significant reduction (p <0.05) of the mitotic index (MI) and were adjudged cytotoxic. HM alone and its mixtures with CP or EMS induced statistically significant genotoxicity (p < 0.05). Mixture of HM (0.016 mg/ml) with CP was not significantly more genotoxic than CP alone (ME 0.57). Each mixture of HM (0.03125, 0.0625 mg/ml) with CP was insignificantly less genotoxic than CP alone with modulatory effect (ME) of -0.14 and -0.01, respectively, which suggested no interaction between HM and CP. Mixtures of HM with EMS induced positive and significant (>2-fold) MEs and each mixture was significantly (p < 0.05) more genotoxic than HM or EMS alone which indicated a synergistic interaction. Sticky chromosomes, chromosome laggards, chromosome fragments, anaphase and telophase bridges, binucleate interphase cells were observed.

Key words: Cytotoxicity, cyclophosphamide, ethyl methanesulfonate (EMS), Genotoxicity; anti-genotoxicity.