Journal of
Medicinal Plants Research

  • Abbreviation: J. Med. Plants Res.
  • Language: English
  • ISSN: 1996-0875
  • DOI: 10.5897/JMPR
  • Start Year: 2007
  • Published Articles: 3550

Full Length Research Paper

Spermostatic activity of Eugenia brejoensis and Myroxylon peruiferum essential oils toward human spermatozoa

José Adelson Alves do Nascimento Junior
  • José Adelson Alves do Nascimento Junior
  • ASCES UNITA, Av. Portugal, 584 – Universitário, 55016-901, Caruaru – Pernambuco, Brazil.
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Clovis Macêdo Bezerra Filho
  • Clovis Macêdo Bezerra Filho
  • Departamento de Bioquímica, Universidade Federal de Pernambuco, 50670-420 Recife, Pernambuco, Brazil.
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Thiago Henrique Florencio de Oliveira
  • Thiago Henrique Florencio de Oliveira
  • ASCES UNITA, Av. Portugal, 584 – Universitário, 55016-901, Caruaru – Pernambuco, Brazil.
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Alexandre Gomes da Silva
  • Alexandre Gomes da Silva
  • Departamento de Antibióticos, Centro de Biociências, Universidade Federal de Pernambuco. Av. Prof. Moraes Rego, 1235 – Cidade Universitária, CEP: 50670-901, Recife, Brazil.
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Patricia Cristina Bezerra-Silva
  • Patricia Cristina Bezerra-Silva
  • Departamento de Química Fundamental, Universidade Federal de Pernambuco, Recife-PE, Brazil
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Patricia Maria Guedes Paiva
  • Patricia Maria Guedes Paiva
  • Departamento de Bioquímica, Universidade Federal de Pernambuco, 50670-420 Recife, Pernambuco, Brazil.
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Marcia Vanusa da Silva
  • Marcia Vanusa da Silva
  • Departamento de Bioquímica, Universidade Federal de Pernambuco, 50670-420 Recife, Pernambuco, Brazil.
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Daniela Maria do Amaral Ferraz Navarro
  • Daniela Maria do Amaral Ferraz Navarro
  • Departamento de Química Fundamental, Universidade Federal de Pernambuco, Recife-PE, Brazil
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Luis Claudio Nascimento da Silva
  • Luis Claudio Nascimento da Silva
  • ASCES UNITA, Av. Portugal, 584 – Universitário, 55016-901, Caruaru – Pernambuco, Brazil.
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Maria Tereza dos Santos Correia
  • Maria Tereza dos Santos Correia
  • Departamento de Bioquímica, Universidade Federal de Pernambuco, 50670-420 Recife, Pernambuco, Brazil.
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  •  Received: 04 June 2018
  •  Accepted: 25 June 2018
  •  Published: 10 July 2018

Abstract

In this study, the in vitro spermostatic action, hemolytic action in combination of parabens of two essential oils (EOs) from Eugenia brejoensis Mazin (Myrtaceae, EbEO) and Myroxylon peruiferum L. (Fabaceae, MpEO) is reported, in addition to the first chemical characterization of MpEO. The EOs were obtained by hydrodistillation and characterized by gas chromatography-mass spectrometry (GC-MS). Different concentrations of both EOs (25 to 400 µg/mL) were mixed with human ejaculate and sperm motility was assessed after 5, 15 and 30 min. A total of 44 compounds were identified in MpEO; the major components were spathulenol, α-pinene, caryophyllene oxide, limonene and β-elemene. Both EOs exhibited spermostatic action in a dose-dependent manner. The best results were obtained for EbEO at all treatment times, showing IC50 values of 454.75 µg/mL (5 min), 417.68 µg/mL (15 min) and 403.93 µg/mL (30 min). M. peruiferum also showed significant immobilizing potential with IC50 values of 620.39 µg/mL (5 min), 652.70 µg/mL (15 min) and 579.37 µg/mL (30 min). Both oils showed low cytotoxicity against human erythrocytes. This is the first report on human spermostatic action for EOs from E. brejoensis and M. peruiferum which has antimicrobial activity and low cytotoxicity. These results suggest the need for further research on toxicological aspects of both EOs and their components in order to develop new pharmaceutical preparations using them as leader compounds.

Key words: Eugenia brejoensis and Myroxylon peruiferum essential oils, spermostatic activity, human spermatozoa.