Journal of Medicinal Plants Research
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Article Number - E7990F620400


Vol.6(4), pp. 566-573 , January 2012
DOI: 10.5897/JMPR10.613
ISSN: 1996-0875
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Full Length Research Paper

Isolation and characterization of antimicrobial activity conferring component(s) from seeds of bitter gourd (Momordica charantia)


Abid Mahmood1, Ghazala Kaukab Raja1, Tariq Mahmood2*, Muhammad Gulfraz1 and

Azra Khanum1




1Department of Biochemistry, University of Arid Agriculture Rawalpindi, Pakistan.

2Department of Plant Sciences, Quaid-i-Azam University, Islamabad-45320, Pakistan.


Email: tmahmood@qau.edu.pk




 Accepted: 22 November 2011  Published: 30 January 2012

Copyright © 2012 Author(s) retain the copyright of this article.
This article is published under the terms of the Creative Commons Attribution License 4.0


Momordica charantia, a commonly used vegetable in Pakistan, has been known for its medicinal importance to cure diabetes but its antimicrobial activity has not been explored yet. Present study was designed to investigate antimicrobial activity of M. charantia seed extracts against mammalian and poultry pathogens. Buffered saline, ethanol, n-hexane, petroleum ether based seed extracts of M. charantia were prepared to screen anti-microbial activity by disc diffusion method. The aqueous seed extract showed highest antimicrobial activity againstPasteurella multocida while ethanol, n-hexane and petroleum ether extracts were effective against Staphylococcus aureus. The protein/peptides were isolated by 80% ammonium sulphate precipitation and gel filtration chromatographyand separated on SDS-PAGE. The electrophoretic profile of dialyzed seed extracts showed seven bands of peptide/protein bands ranging from ~9 to ~54 kDa. The protein/peptides seed extract was efficient against S. aureusand Salmonella typhi with the maximum zone of inhibition (1 cm). All results were compared with chloramphenicol, an antibiotic with highest antimicrobial activity against all tested microorganisms.

 

Key words: Bitter gourd, antimicrobial peptides, chromatography.


APA (2012). Isolation and characterization of antimicrobial activity conferring component(s) from seeds of bitter gourd (Momordica charantia). Journal of Medicinal Plants Research, 6(4), 566-573.
Chicago Abid Mahmood, Ghazala Kaukab Raja, Tariq Mahmood, Muhammad Gulfraz and Azra Khanum. "Isolation and characterization of antimicrobial activity conferring component(s) from seeds of bitter gourd (Momordica charantia)." Journal of Medicinal Plants Research 6, no. 4 (2012): 566-573.
MLA Abid Mahmood, et al. "Isolation and characterization of antimicrobial activity conferring component(s) from seeds of bitter gourd (Momordica charantia)." Journal of Medicinal Plants Research 6.4 (2012): 566-573.
   
DOI 10.5897/JMPR10.613
URL http://www.academicjournals.org/journal/JMPR/article-abstract/E7990F620400

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