Full Length Research Paper
Protective effects of Ziziphus jujuba fruit extract against ethanol-induced hippocampal oxidative stress and spatial memory impairment in rats
Majid Taati1*, Masoud Alirezaei2, Mohamad Hadi Moshkatalsadat3, Bahram Rasoulian4, Mehrnoush Moghadasi4, Farzam Sheikhzadeh5 and Ali Sokhtezari6
1Department of Physiology, School of Veterinary Medicine, Lorestan University, Khoramabad, Iran.
2Division of Biochemistry, School of Veterinary Medicine, Lorestan University, Khoramabad, Iran.
3Department of Chemistry, School of Basic Sciences, Lorestan University, Khoramabad, Iran.
4Razi Herbal Medicines Research Center, Lorestan University of Medical Sciences, Khoramabad, Iran.
5Department of Biotechnology, Agriculture Faculty, Tabriz University, Iran.
6Department of Clinical Sciences, School of Veterinary Medicine, Lorestan University, Khoramabad, Iran.
Accepted: 15 February 2011 Published: 18 March 2011
The current study assessed the effects of Ziziphus jujuba fruit extract on hippocampal oxidative stress and spatial memory of rats exposed to ethanol. Male Wistar rats were randomly divided into control, Z. jujuba treated (200 mg/kg, p.o.), ethanol (4 g/kg, p.o.) andZ. jujuba plus ethanol groups. The animals were treated daily by oral gavage for 8 weeks. The learning and memory performance was assessed using Morris water maze (from day 55 to 60). At the end of experiment, rats were sacrificed by decapitation and brains were dissected out for thiobarbituric acid reactive substances, and the activities of antioxidative enzymes (glutathione peroxidase and superoxide dismutase) determinations in the hippocampus. It was found that combined Z. jujuba extract-ethanol treatment significantly decreased TBARS level and increased glutathione peroxidase activity compared to the ethanol group (p<0.05), whereas no significant difference was observed in superoxide dismutase activity. In Morris water maze, rats treated with Z. jujuba prior to ethanolsignificantly shortened total path length during the acquisition period and enhanced the spent time in the correct quadrant on probe trial performance compared to ethanol treated rats (p<0.05). The results show that Z. jujuba fruit extract improved spatial memory impairment induced by ethanol, due in part, by its antioxidant activities.
Key words: Zizyphus jujuba, ethanol, oxidative stress, memory.