The global interest in natural substances has increased. Three Ballerina (TB) is a natural formula that has medicinal properties. Scientific information about this formula is not found in the PubMed and this fact has stimulated investigations about its biological effects. An experimental model using a reducing agent, stannous chloride (SnCl2), was carried out. Although, SnCl2 is used in nuclear medicine to obtain technetium-99 m radiopharmaceuticals, it is capable to generate free radicals. The aim of this work was to evaluate the biological effects of an aqueous extract of TB on the survival of Escherichia coli (E. coli) AB1157 (wild type) cultures and to verify if this extract protect a E. coli culture against the action of SnCl2. E. coli AB1157 cultures (exponential growth phase) were incubated with TB extract (23.4 mg/ml) or 0.9% NaCl solution as a control. E. coli cultures were also incubated with SnCl2 (25 µg/ml) + TB extract (23.4 mg/ml) or SnCl2 (25 µg/ml) alone. Aliquots from these treatments were spread onto Petri dishes containing solidified LB medium. The colony-forming units (CFU) were counted after overnight and the survival fractions calculated. Data reveal that the TB did not alter the survival of the E. coli culture, however, it has protected the cells against the lethal effect of the SnCl2. In conclusion, the TB extract did not present cytotoxic effects, but it appears to have redox action.
Key words: Escherichia coli, three ballerina, stannous chloride, cytotoxic effect.
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