Journal of Medicinal Plants Research
Subscribe to JMPR
Full Name*
Email Address*

Article Number - 21A259C45364


Vol.8(9), pp. 408-415 , March 2014
DOI: 10.5897/JMPR2013.5044
ISSN: 1996-0875



Full Length Research Paper

The influence of extraction solvents on the anticancer activities of Palestinian medicinal plants



Jawad Alzeer
  • Jawad Alzeer
  • Applied Chemistry Department, Palestine Polytechnic University, Palestine.
  • Google Scholar
Balayeshwanth R. Vummidi
  • Balayeshwanth R. Vummidi
  • Institute of Organic Chemistry, University of Zürich, Switzerland.
  • Google Scholar
Rami Arafeh
  • Rami Arafeh
  • Applied Chemistry Department, Palestine Polytechnic University, Palestine.
  • Google Scholar
Waleed Rimawi
  • Waleed Rimawi
  • Applied Chemistry Department, Palestine Polytechnic University, Palestine.
  • Google Scholar
Hatem Saleem
  • Hatem Saleem
  • Applied Chemistry Department, Palestine Polytechnic University, Palestine.
  • Google Scholar
Nathan W. Luedtke
  • Nathan W. Luedtke
  • Institute of Organic Chemistry, University of Zürich, Switzerland.
  • Google Scholar







 Accepted: 11 February 2014  Published: 03 March 2014

Copyright © 2014 Author(s) retain the copyright of this article.
This article is published under the terms of the Creative Commons Attribution License 4.0


Palestine has a rich and prestigious heritage of herbal medicines. To investigate the impact of variable extraction techniques on the cytotoxic effects of medicinal plant extracts, 5 well-known medicinal plants from Palestine were extracted with 90% ethanol, 80% methanol, acetone, coconut water, apple vinegar, grape vinegar or 5% acetic acid. The resulting 35 extracts were screened for cytotoxic activities against three different cancer cell lines (B16F10, MCF-7 and HeLa) using a standard resazurin-based cytotoxicity assay and Nile Blue A as the positive control. Highly variable toxicities and tissue sensitivity were observed, depending upon the solvent used for extraction. The acetone extract of Salvia officinalis L. exhibited the most potent cytotoxicity (IC50 = 14 to 36 µg/ml), but very little sensitivity between the three cell lines. More moderate cytotoxicity with improved tissue sensitivity was observed with coconut water extract of S. officinalis L. (IC50 = 114 µg/ml) and methanol extract of Teucrium polium L. (IC50 = 104 µg/ml). In this study, acetone consistently gave lower extraction yields but higher cytotoxicity, whereas other solvent systems gave much higher extraction yields with lower cytotoxicity. These results demonstrate how the cytotoxicity of plant extracts can be inversely proportional to the yield, and that solvent selection plays an important role in both factors.

 

Kew words: Plant extract, natural products, anticancer drug, cytotoxicity.

 

Ali-Shtayeh MS, Jamous RM (2012). Complementary and alternative medicine use amongst Palestinian diabetic patients. Complement. Ther. Clin. Pract. 18:16-21.
Crossref
 
Ali-Shtayeh MS, Yaghmour RM, Faidi YR, Salem K, Al-Nuri MA (1998). Antimicrobial activity of 20 plants used in folkloric medicine in the Palestinian area. J. Ethnopharmacol. 60:265-271.
Crossref
 
Alu'datt MH, Alli I, Ereifej K, Alhamad MN, Alsaad A, Rababeh T (2011). Optimisation and characterisation of various extraction conditions of phenolic compounds and antioxidant activity in olive seeds. Nat. Prod. Res. 25:876-889.
Crossref
 
Aron PM, Kennedy JA (2007). Compositional investigation of phenolic polymers isolated from Vitis vinifera L. Cv. Pinot Noir during fermentation. J. Agric. Food Chem. 55:5670-5680.
Crossref
 
Baluchnejadmojarad T, Roghani M, Roghani-Dehkordi F (2005). Antinociceptive effect of Teucrium polium leaf extract in the diabetic rat formalin test. J. Ethnopharmacol. 97:207-210.
Crossref
 
Chemat F, Vian MA, Cravotto G (2012). Green extraction of natural products: concept and principles. Int. J. Mol. Sci. 13:8615-8627.
Crossref
 
Conforti F, Menichini G, Zanfini L, Tundis R, Statti GA, Provenzano E, Menichini F, Somma F, Alfano C (2012). Evaluation of phototoxic potential of aerial components of the fig tree against human melanoma. Cell Prolif. 45:279-285.
Crossref
 
Daoudi A, El Yoube A, Bagrel D, Aarab L (2013). In vitro anticancer activity of some plants used in Moroccan traditional medicine. J. Med. Plants Res. 7:1182-1189.
 
Diaz-Reinoso B, Moure A, Dominguez H, Parajo JC (2006). Supercritical CO2 extraction and purification of compounds with antioxidant activity. J. Agric. Food Chem. 54:2441-2469.
Crossref
 
Efferth T (2009). Natural products pave their way in cancer therapy. Cancer Biol. Ther. 8:1869-1870.
View
 
Efferth T (2010). Cancer therapy with natural products and medicinal plants. Planta Med. 76:1035-1036.
Crossref
 
Eidi A, Eidi M, Darzi R (2009). Antidiabetic effect of Olea europaea L. in normal and diabetic rats. Phytother. Res. 23:347-350.
Crossref
 
El Hadri A, Río M, Sanz J, González Coloma A, Idaomar M, Ribas Ozonas B, Benedí González J, Isabel Sánchez Reus M (2010). Cytotoxic activity of a-humulene and transcaryophyllene from Salvia officinalis in animal and human tumor cells. An. R. Acad. Nac. Farm. 76:343-356.
 
Filip A, Daicoviciu D, Clichici S, Mocan T, Muresan A, Postescu ID (2011). Photoprotective effects of two natural products on ultraviolet B-induced oxidative stress and apoptosis in SKH-1 mouse skin. J. Med. Food 14:761-766.
Crossref
 
Fontana AR, Wuilloud RG, Martinez LD, Altamirano JC (2009). Simple approach based on ultrasound-assisted emulsification-microextraction for determination of polibrominated flame retardants in water samples by gas chromatography-mass spectrometry. J. Chromatogr. A. 1216:147-153.
Crossref
 
Hait WN (2009). Targeted cancer therapeutics. Cancer Res. 69:1263-1267; discussion 1267.
Crossref
 
Hassan MM, Muhtadi FJ, Al-Badr AA (1979). GLC-mass spectrometry of Teucrium polium oil. J. Pharm. Sci. 68:800-801.
Crossref
 
Kaileh M, Vanden Berghe W, Boone E, Essawi T, Haegeman G (2007). Screening of indigenous Palestinian medicinal plants for potential anti-inflammatory and cytotoxic activity. J. Ethnopharmacol. 113:510-516.
Crossref
 
Kontogianni VG, Gerothanassis IP (2012). Phenolic compounds and antioxidant activity of olive leaf extracts. Nat. Prod. Res. 26:186-189.
Crossref
 
Lin CW, Shulok JR, Kirley SD, Cincotta L, Foley JW (1991). Lysosomal localization and mechanism of uptake of Nile blue photosensitizers in tumor cells. Cancer Res. 51:2710-2719.
Pubmed
 
Lippert TH, Ruoff HJ, Volm M (2008). Intrinsic and acquired drug resistance in malignant tumors. The main reason for therapeutic failure. Arzneimittelforschung 58:261-264.
Pubmed
 
Loizzo MR, Tundis R, Menichini F, Saab AM, Statti GA (2007). Cytotoxic activity of essential oils from labiatae and lauraceae families against in vitro human tumor models. Anticancer Res. 27:3293-3299.
Pubmed
 
Min B, Lim J, Ko S, Lee KG, Lee SH, Lee S (2011). Environmentally friendly preparation of pectins from agricultural byproducts and their structural/rheological characterization. Bioresour. Technol. 102:3855-3860.
Crossref
 
Mukherjee AK, Basu S, Sarkar N, Ghosh AC (2001). Advances in cancer therapy with plant based natural products. Curr. Med. Chem. 8:1467-1486.
Crossref
 
O'Brien J, Wilson I, Orton T, Pognan F (2000). Investigation of the Alamar (resazurin) fluorescent dye for the assessment of mammalian cell cytotoxicity. Eur. J. Biochem. 267:5421-5426.
Crossref
 
Orhan N, Aslan M, Orhan DD, Ergun F, Yesilada E (2006). In-vivo assessment of antidiabetic and antioxidant activities of grapevine leaves (Vitis vinifera) in diabetic rats. J. Ethnopharmacol. 108:280-286.
Crossref
 
Petrelli A, Giordano S (2008). From single- to multi-target drugs in cancer therapy: when a specificity becomes an advantage. Curr. Med. Chem. 15:422-432.
Crossref
 
Raymond E (2004). Are natural products still of interest compared with molecular-targeted therapy for the treatment of cancer? Clin. Lung Cancer 5:313.
Crossref
 
Sawalha AF, Sweileh WM, Zyoud SH, Jabi SW (2008). Self-therapy practices among university students in Palestine: focus on herbal remedies. Complement. Ther. Med. 16:343-349.
Crossref
 
Siu D (2011). Natural products and their role in cancer therapy. Med. Oncol. 28:888-900.
Pubmed
 
Suffness M, Pezzuto JM (1990). Assays related to cancer drug discovery, In: Hostettmann, K. (Ed). Methods Plant Biochemistry: Assays for Bioactivity. London: Academic Press 6:71-133.
 
Suleiman MS, Abdul-Ghani AS, Al-Khalil S, Amin R (1988). Effect of Teucrium polium boiled leaf extract on intestinal motility and blood pressure. J. Ethnopharmacol. 22:111-116.
Crossref
 
Surh YJ, Chun KS, Cha HH, Han SS, Keum YS, Park KK, Lee SS (2001). Molecular mechanisms underlying chemopreventive activities of anti-inflammatory phytochemicals: down-regulation of COX-2 and iNOS through suppression of NF-kappa B activation. Mutat. Res. 480-481:243-268.
Crossref
 
Taamalli A, Arraez-Roman D, Barrajon-Catalan E, Ruiz-Torres V, Perez-Sanchez A, Herrero M, Ibanez E, Micol V, Zarrouk M, Segura-Carretero A, Fernandez-Gutierrez A (2012). Use of advanced techniques for the extraction of phenolic compounds from Tunisian olive leaves: phenolic composition and cytotoxicity against human breast cancer cells. Food Chem. Toxicol. 50:1817-1825.
Crossref
 
Waterman E, Lockwood B (2007). Active components and clinical applications of olive oil. Altern. Med. Rev. 12:331-342.
Pubmed

 


APA Alzeer, J., Vummidi, B. R., Arafeh, R., Rimawi, W., Saleem, H., & Luedtke, N. W. (2014). The influence of extraction solvents on the anticancer activities of Palestinian medicinal plants. Journal of Medicinal Plants Research, 8(9), 408-415.
Chicago Jawad Alzeer, Balayeshwanth R. Vummidi, Rami Arafeh, Waleed Rimawi, Hatem Saleem and Nathan W. Luedtke. "The influence of extraction solvents on the anticancer activities of Palestinian medicinal plants." Journal of Medicinal Plants Research 8, no. 9 (2014): 408-415.
MLA Jawad Alzeer, et al. "The influence of extraction solvents on the anticancer activities of Palestinian medicinal plants." Journal of Medicinal Plants Research 8.9 (2014): 408-415.
   
DOI 10.5897/JMPR2013.5044
URL http://www.academicjournals.org/journal/JMPR/article-abstract/21A259C45364

Subscription Form