Journal of
Medicinal Plants Research

  • Abbreviation: J. Med. Plants Res.
  • Language: English
  • ISSN: 1996-0875
  • DOI: 10.5897/JMPR
  • Start Year: 2007
  • Published Articles: 3553

Full Length Research Paper

Isolation, characterization and biological activity of organic extractives from Calodendrum capense (L.f.) Thunb.(Rutaceae)

Okwemba R. I.
  • Okwemba R. I.
  • Chemistry Department, School of Pure and Applied Chemistry, Kenyatta University, P. O. Box 43844, Nairobi, Kenya.
  • Google Scholar
Tarus P. K.
  • Tarus P. K.
  • Department of Chemistry and Biochemistry, University of Eldoret, P. O. Box 1125 - 30100, Eldoret, Kenya.
  • Google Scholar
Machocho A. K.
  • Machocho A. K.
  • Chemistry Department, School of Pure and Applied Chemistry, Kenyatta University, P. O. Box 43844, Nairobi, Kenya.
  • Google Scholar
Wanyonyi A. W.
  • Wanyonyi A. W.
  • Chemistry Department, School of Pure and Applied Chemistry, Kenyatta University, P. O. Box 43844, Nairobi, Kenya.
  • Google Scholar
Waweru, I. M.
  • Waweru, I. M.
  • Chemistry Department, School of Pure and Applied Chemistry, Kenyatta University, P. O. Box 43844, Nairobi, Kenya.
  • Google Scholar
Onyancha J. M.
  • Onyancha J. M.
  • Department of Pharmacy and Complementary/Alternative medicine, School of Medicine, Kenyatta University, P. O. Box 43844, Nairobi, Kenya.
  • Google Scholar
Onani M. O.
  • Onani M. O.
  • Department of Chemistry, Faculty of Natural Science, University of the Western Cape, Modderdam Road, Private bag X1, Belleville, South Africa.
  • Google Scholar
Amuka O.
  • Amuka O.
  • Department of Applied Sciences, Maseno University, Private Bag Maseno, Kenya.
  • Google Scholar


  •  Received: 27 December 2017
  •  Accepted: 03 April 2018
  •  Published: 05 April 2018

Abstract

A novel prenylfuroquinoline alkaloid capensenin (1), an alkaloid confusameline (2), two furanocoumarins psolaren (3) and bergapten (4), were isolated from hexane and dichloromethane crude extracts of stem bark, leaves and fruit pericarp of Calodendrum capense. Limonin (5) was also isolated from the stem bark, while limonin diosphenol (6) was isolated from the seeds. Capensenin (1) showed weak antimicrobial activity against Bacillus subtilis, while the leaves, stem bark and fruit pericarp crude extracts exhibited activity against Staphylococcus aureus, B. subtilis and P. citrinum. Hexane pericarp extract showed slight cytotoxicity to Vero cell E199 in 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay. The structures of the compounds were elucidated by 1D and 2D nuclear magnetic resonance (NMR) spectroscopy, mass spectrometry and infra-red spectroscopy.

Key words: Capensenin, 4-hydroxyfuroquinoline, furanocoumarin, Calodendrum capense, alkaloid, Rutaceae.