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Article Number - 0EEE8A649607


Vol.8(48), pp. 1408-1417 , December 2014
DOI: 10.5897/JMPR2014.5688
ISSN: 1996-0875



Full Length Research Paper

In vitro antibacterial action on methicillin-susceptible (MSSA) and methicillin-resistant (MRSA) Staphylococcus aureus and antitumor potential of Mauritia flexuosa L. f.



Ezequias Pessoa de Siqueira*
  • Ezequias Pessoa de Siqueira*
  • René Rachou Research Center, Oswaldo Cruz Foundation, Av. Augusto de Lima, 1715, 30190-002, Belo Horizonte, Minas Gerais, Brazil.
  • Google Scholar
Anderson Assunção Andrade2
  • Anderson Assunção Andrade2
  • Laboratory of Microbiology, Institute of Biological and Natural Sciences, Federal University of Triangulo Mineiro, Praça Manoel Terra, 330, 38025-015, Uberaba, MG, Brazil.
  • Google Scholar
Elaine Maria de Souza-Fagundes3,
  • Elaine Maria de Souza-Fagundes3,
  • Department of Physiology and Biophysics, Institute of Biological Sciences, Federal University of Minas Gerais, Av. Antonio Carlos, 6627/3, 31270-901, Belo Horizonte, Minas Gerais, Brazil.
  • Google Scholar
Jonas Pereira Ramos
  • Jonas Pereira Ramos
  • Department of Physiology and Biophysics, Institute of Biological Sciences, Federal University of Minas Gerais, Av. Antonio Carlos, 6627/3, 31270-901, Belo Horizonte, Minas Gerais, Brazil.
  • Google Scholar
Markus Kohlhoff
  • Markus Kohlhoff
  • René Rachou Research Center, Oswaldo Cruz Foundation, Av. Augusto de Lima, 1715, 30190-002, Belo Horizonte, Minas Gerais, Brazil.
  • Google Scholar
Yule Roberta Ferreira Nunes
  • Yule Roberta Ferreira Nunes
  • Ecology Laboratory and Plant Propagation, Department of General Biology, Estadual University of Montes Claros, University Campus Prof. Darcy Ribeiro, 39401-089, Montes Claros, Minas Gerais, Brazil.
  • Google Scholar
Maria das Dores Magalhães Veloso
  • Maria das Dores Magalhães Veloso
  • Ecology Laboratory and Plant Propagation, Department of General Biology, Estadual University of Montes Claros, University Campus Prof. Darcy Ribeiro, 39401-089, Montes Claros, Minas Gerais, Brazil.
  • Google Scholar
Fernanda Fraga Campos
  • Fernanda Fraga Campos
  • Federal University of the Valleys of the Jequitinhonha and Mucuri, Department of Biological Sciences and Health, Rua da Glória, 187, 39100-000, Diamantina, Brazil.
  • Google Scholar
Susana Johann
  • Susana Johann
  • Department of Microbiology, Federal University of Minas Gerais, Av. Presidente Antonio Carlos, 6627/3, 31270-901, Belo Horizonte, Minas Gerais, Brazil.
  • Google Scholar
Tânia Maria de Almeida Alves
  • Tânia Maria de Almeida Alves
  • René Rachou Research Center, Oswaldo Cruz Foundation, Av. Augusto de Lima, 1715, 30190-002, Belo Horizonte, Minas Gerais, Brazil.
  • Google Scholar
Carlos Leomar Zani
  • Carlos Leomar Zani
  • René Rachou Research Center, Oswaldo Cruz Foundation, Av. Augusto de Lima, 1715, 30190-002, Belo Horizonte, Minas Gerais, Brazil.
  • Google Scholar
Betania Barros Cota
  • Betania Barros Cota
  • René Rachou Research Center, Oswaldo Cruz Foundation, Av. Augusto de Lima, 1715, 30190-002, Belo Horizonte, Minas Gerais, Brazil.
  • Google Scholar







 Received: 12 November 2014  Accepted: 16 December 2014  Published: 25 December 2014

Copyright © 2014 Author(s) retain the copyright of this article.
This article is published under the terms of the Creative Commons Attribution License 4.0


Mauritia flexuosa L.f. (Arecaceae), commonly known as the buriti palm, is used in traditional medicine in Brazil. Oil from fruits is used for wound healing of skin burns and as an anti rheumatic agent. The stem pith is used to treat dysentery and diarrhea. The aim of this study was to evaluate the in vitro antibacterial and cytotoxic activities of M. flexuosa L.f. extracts and fractions. Crude ethanol extracts (EtOH) from fruits, leaves, and stems were submitted to liquid-liquid partition to yield four different soluble fractions: hexane (Hex), dichloromethane (DCM), ethyl acetate (EtOAc) and aqueous (Aq). The antibacterial activity was evaluated using the broth micro dilution method against methicillin-susceptible S. aureus (MSSA - ATCC 29213) and methicillin-resistant S. aureus (MRSA – clinical sample 155). Cytotoxicity activity was determined against five human tumor cell lines (HL-60, Jurkat, THP-1, MCF-7, and HCT-116) and Vero cell as a model for toxicity control, using MTT colorimetric assay. Across all extracts and fractions assayed, only DCM fractions from leaves and stems were active against S. aureus (MSSA and MRSA) at concentrations ranging from 31.3 to 62.5 µg/ml, and against tumor cell lines with IC50 values ranging from 20.3 to 79.0 µg/ml. The DCM fraction from stems was purified by gel permeation chromatography (GPC) to yield G1 to G12 groups. Groups G7 to G12 showed antimicrobial activity against MSSA and MRSA with MIC values of 31.3 to 125.0 µg/ml and four groups (G3, G9, G10, G12) exhibited cytotoxic activities in the range of 20 µg/ml against at least one tumor cell line. Using the hyphenated methodology, liquid chromatography-mass spectrometry (LC-MS), it was possible to identify several phenolic compounds in subgroups G7 to G10 that can be associated with the medicinal properties of stems from buriti.

 

Key words: Mauritia flexuosa, antibacterial activity, antitumoral activity, phenolic compounds.

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APA de Siqueira*, E. P., Andrade, A. A., de Souza-Fagundes, E. M., Ramos, J. P., Kohlhoff, M., Nunes, Y. R. F., Veloso, M. D. M., Campos, F. F., Johann, S., Alves, T. M. A., Zani, C. L., & Cota, B. B. (2014). In vitro antibacterial action on methicillin-susceptible (MSSA) and methicillin-resistant (MRSA) Staphylococcus aureus and antitumor potential of Mauritia flexuosa L. f.. Journal of Medicinal Plants Research, 8(48), 1408-1417.
Chicago Ezequias Pessoa de Siqueira, Anderson Assunção Andrade, Elaine Maria de Souza-Fagundes, Jonas Pereira Ramos, Markus Kohlhoff, Yule Roberta Ferreira Nunes, Maria das Dores Magalhães Veloso, Fernanda Fraga Campos, Susana Johann, Tânia Maria de Almeida Alves, Carlos Leomar Zani and Betania Barros Cota. "In vitro antibacterial action on methicillin-susceptible (MSSA) and methicillin-resistant (MRSA) Staphylococcus aureus and antitumor potential of Mauritia flexuosa L. f.." Journal of Medicinal Plants Research 8, no. 48 (2014): 1408-1417.
MLA Ezequias Pessoa de Siqueira, et al. "In vitro antibacterial action on methicillin-susceptible (MSSA) and methicillin-resistant (MRSA) Staphylococcus aureus and antitumor potential of Mauritia flexuosa L. f.." Journal of Medicinal Plants Research 8.48 (2014): 1408-1417.
   
DOI 10.5897/JMPR2014.5688
URL http://www.academicjournals.org/journal/JMPR/article-abstract/0EEE8A649607

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21SELECT `Setting`.`id`, `Setting`.`key`, `Setting`.`value`, `Setting`.`description`, `Setting`.`created`, `Setting`.`modified` FROM `admin_academic_journals`.`settings` AS `Setting` WHERE 1 = 1660
22SELECT `Setting`.`id`, `Setting`.`key`, `Setting`.`value`, `Setting`.`description`, `Setting`.`created`, `Setting`.`modified` FROM `admin_academic_journals`.`settings` AS `Setting` WHERE 1 = 1660
23SELECT `Setting`.`id`, `Setting`.`key`, `Setting`.`value`, `Setting`.`description`, `Setting`.`created`, `Setting`.`modified` FROM `admin_academic_journals`.`settings` AS `Setting` WHERE 1 = 1660
24SELECT `JournalIssue`.`id`, `JournalIssue`.`journal_id`, `JournalIssue`.`archive_id`, `JournalIssue`.`volume_number`, `JournalIssue`.`issue_number`, `JournalIssue`.`page_range`, `JournalIssue`.`edition`, `JournalIssue`.`month`, `JournalIssue`.`picture`, `JournalIssue`.`home_issue`, `JournalIssue`.`current_issue`, `JournalIssue`.`articles_count`, `JournalIssue`.`e-book`, `JournalIssue`.`issuu_embed`, `JournalIssue`.`subject`, `JournalIssue`.`created`, `JournalIssue`.`modified` FROM `admin_academic_journals`.`journal_issues` AS `JournalIssue` WHERE `JournalIssue`.`id` = 6484 LIMIT 1110
25SELECT `Setting`.`id`, `Setting`.`key`, `Setting`.`value`, `Setting`.`description`, `Setting`.`created`, `Setting`.`modified` FROM `admin_academic_journals`.`settings` AS `Setting` WHERE 1 = 1660
26SELECT `JournalIssue`.`id`, `JournalIssue`.`journal_id`, `JournalIssue`.`archive_id`, `JournalIssue`.`volume_number`, `JournalIssue`.`issue_number`, `JournalIssue`.`page_range`, `JournalIssue`.`edition`, `JournalIssue`.`month`, `JournalIssue`.`picture`, `JournalIssue`.`home_issue`, `JournalIssue`.`current_issue`, `JournalIssue`.`articles_count`, `JournalIssue`.`e-book`, `JournalIssue`.`issuu_embed`, `JournalIssue`.`subject`, `JournalIssue`.`created`, `JournalIssue`.`modified` FROM `admin_academic_journals`.`journal_issues` AS `JournalIssue` WHERE `JournalIssue`.`id` = 6484 LIMIT 1110
27SELECT `Setting`.`id`, `Setting`.`key`, `Setting`.`value`, `Setting`.`description`, `Setting`.`created`, `Setting`.`modified` FROM `admin_academic_journals`.`settings` AS `Setting` WHERE 1 = 1660
28SELECT `Setting`.`id`, `Setting`.`key`, `Setting`.`value`, `Setting`.`description`, `Setting`.`created`, `Setting`.`modified` FROM `admin_academic_journals`.`settings` AS `Setting` WHERE 1 = 1660
29SELECT `Setting`.`id`, `Setting`.`key`, `Setting`.`value`, `Setting`.`description`, `Setting`.`created`, `Setting`.`modified` FROM `admin_academic_journals`.`settings` AS `Setting` WHERE 1 = 1660
30SELECT `Setting`.`id`, `Setting`.`key`, `Setting`.`value`, `Setting`.`description`, `Setting`.`created`, `Setting`.`modified` FROM `admin_academic_journals`.`settings` AS `Setting` WHERE 1 = 1660
31SELECT `Setting`.`id`, `Setting`.`key`, `Setting`.`value`, `Setting`.`description`, `Setting`.`created`, `Setting`.`modified` FROM `admin_academic_journals`.`settings` AS `Setting` WHERE 1 = 1660
32SELECT `Logo`.`id`, `Logo`.`name`, `Logo`.`banner`, `Logo`.`description`, `Logo`.`created`, `Logo`.`modified` FROM `admin_academic_journals`.`logos` AS `Logo` WHERE `Logo`.`id` = 1 LIMIT 1110
33SELECT `Setting`.`id`, `Setting`.`key`, `Setting`.`value`, `Setting`.`description`, `Setting`.`created`, `Setting`.`modified` FROM `admin_academic_journals`.`settings` AS `Setting` WHERE 1 = 1660
34SELECT `Journal`.`id`, `Journal`.`journal_category_id`, `Journal`.`name`, `Journal`.`issn`, `Journal`.`abbrev`, `Journal`.`pub_abbrev`, `Journal`.`banner`, `Journal`.`colour`, `Journal`.`email`, `Journal`.`password`, `Journal`.`instruction_for_author_file`, `Journal`.`reviewers_guide_file`, `Journal`.`about_text`, `Journal`.`submit_manuscript`, `Journal`.`instructions_for_authors`, `Journal`.`pub_ethics`, `Journal`.`abstracting`, `Journal`.`call_for_papers`, `Journal`.`faculty_1000`, `Journal`.`editors`, `Journal`.`journal_issue_count`, `Journal`.`journ_handling_fee`, `Journal`.`founding_editor`, `Journal`.`founding_editor_address`, `Journal`.`created`, `Journal`.`modified`, `Journal`.`subdomain` FROM `admin_academic_journals`.`journals` AS `Journal` WHERE `Journal`.`id` = 7 LIMIT 1110