Journal of Agricultural Biotechnology and Sustainable Development
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Article Number - 0C99BDA1770


Vol.3(9), pp. 182-188 , November 2011

ISSN: 2141-2340


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Full Length Research Paper

Modification of surface structure and crystallinity of water hyacinth (Eichhornia crassipes) following recombinant α-L-arabinofuranosidase (abfa) treatment


Anita Kurniati1, Handoko Darmokoesoemo2 and N. N. T. Puspaningsih1,2*




 

1Proteomic Laboratory, Institute of Tropical Disease, Airlangga University, Campus C Mulyorejo, Surabaya, East Java 60115, Indonesia.

2Department of Chemistry, Faculty of Science and Technology, Airlangga University, Campus C Mulyorejo, Surabaya, East Java 60115, Indonesia.


Email: [email protected]






 Accepted: 06 September 2011  Published: 30 November 2011

Copyright © 2011 Author(s) retain the copyright of this article.
This article is published under the terms of the Creative Commons Attribution License 4.0


 

This research was aimed to investigate the modification of surface structure and crystallinity of water hyacinth after recombinant α-L-arabinofuranosidase treatment. The process of water hyacinth hydrolysis was optimized first, by determination of the most optimal intracellular enzyme (P) and extracellular enzyme (S) mixture ratio, and its incubation time. The optimum of water hyacinth hydrolysis process was achieved at ratio P:S = 1:2, after 8 h incubation. The surface structure of water hyacinth after recombinant α-L-arabinofuranosidase treatment was analyzed by scanning electron microscope (SEM) and X-ray diffraction (XRD), using untreated water hyacinth as control. This research showed that enzymatic hydrolysis damaged the surface structure of water hyacinth and changed its crystallinity. The enzyme used in this research can be applied further for bio-bleaching of paper pulp and animal feed stock, while the hydrolysis product can be applied for bioethanol production.  

 

Key words: Water hyacinth, α-L-arabinofuranosidase, scanning electron microscope, X-ray diffraction.


APA (2011). Modification of surface structure and crystallinity of water hyacinth (Eichhornia crassipes) following recombinant α-L-arabinofuranosidase (abfa) treatment. Journal of Agricultural Biotechnology and Sustainable Development, 3(9), 182-188.
Chicago Anita Kurniati, Handoko Darmokoesoemo and N. N. T. Puspaningsih,. "Modification of surface structure and crystallinity of water hyacinth (Eichhornia crassipes) following recombinant α-L-arabinofuranosidase (abfa) treatment." Journal of Agricultural Biotechnology and Sustainable Development 3, no. 9 (2011): 182-188.
MLA Anita Kurniati, et al. "Modification of surface structure and crystallinity of water hyacinth (Eichhornia crassipes) following recombinant α-L-arabinofuranosidase (abfa) treatment." Journal of Agricultural Biotechnology and Sustainable Development 3.9 (2011): 182-188.
   
DOI https://doi.org/
URL http://www.academicjournals.org/journal/JABSD/article-abstract/0C99BDA1770

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