January 2010

Real-time quantitative (PCR) applications to quantify and the expression profiles of heat shock protein (HSP70) genes in Nile tilapia, Oreochromis niloticus (L.) and Oreochromis mossambicus (P.)

Quantitative real-time PCR (qRT-PCR) has already been used to study the expression profiles of heat shock protein (Hsp) genes in Nile tilapia, Oreochromisniloticus (Lineaus, 1752) and Oreochromis mossambicu (Peter, 1983). Young fish were exposed to heat stress for 5.5 h followed by qRT-PCR of Hsp70 mRNA, using tubulin (tub) as a reference gene and flourogenic dyes. Expression of Hsp70 mRNA peaked at...

Author(s): J. Baby Joseph and S. S. Sujatha

January 2010

Reproductive performance, egg and larval quality and egg fatty acid composition of hatchery-reared Spotted Babylon (Babylonia areolata) broodstock fed natural and formulated diets under hatchery conditions

A 120 day feeding trial was conducted to evaluate the reproductive performance, egg and larval quality and egg fatty acid composition in Spotted Babylon (Babylonia areolata) broodstock fed with natural food (fresh meat of carangid fish, Seleroidesleptolepis,) and one of four experimental formulated diets containing 5 or 15% of dietary lipid from either tuna oil or a mixture of tuna oil and soybean oil (6:4) labeled...

Author(s): S. Sangsawangchote, N. Chaitanawisuti and S. Piyatiratitivorakul