International Journal of Biodiversity and Conservation
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Article Number - FAFA5E755531


Vol.10(2), pp. 52-91 , February 2018
https://doi.org/10.5897/IJBC2017.1146
ISSN: 2141-243X


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Full Length Research Paper

Intra and inter specific genetic divergence of termites species based on ISSR markers and 28S rDNA sequences



Ballagere Puttaraju Harini
  • Ballagere Puttaraju Harini
  • Department of Zoology, Bangalore University, Jnanabharathi Campus, Bengaluru-560 056, Karnataka, India.
  • Google Scholar
Muduvadi Krishnamurthy Pranesh
  • Muduvadi Krishnamurthy Pranesh
  • Centre for Applied Genetics, Bangalore University, Jnanabharathi Campus, Bengaluru-560 056, Karnataka, India.
  • Google Scholar







 Received: 04 September 2017  Accepted: 07 November 2017  Published: 28 February 2018

Copyright © 2018 Author(s) retain the copyright of this article.
This article is published under the terms of the Creative Commons Attribution License 4.0


The comprehensive analysis of five identified and two unknown termite species with their binary scores of three markers viz., morphological, ISSR scoring and 28s rDNA sequence has been made to reveal in unweighted pair group method with arithmetic mean (UPGMA) cluster that Trinervitermes biformis (Wasmann) is out grouped with 35.7% of dissimilarity with other species; Odontotermes obesus (Rambur) and Odontotermes redemanni (Wasmann) share same cluster with 32.7% of dissimilarity; similarly Odontotermes ceylonicus (Wasmann) and Odontotermes horni (Wasmann) share a maximum of 32.5% of dissimilarity. The UNKNOWN01 had more similarity with O. obesus (Rambur) and O. obesus (Rambur) in turn showed high similarity with that of O. redemanni (Wasmann). UNKNOWN02 was found along with O. ceylonicus (Wasmann) and O. horni (Wasmann). The study imparts cumulative or comprehensive analysis using different markers such as morphological, ISSR and nuclear genes like 28s rDNA that depict accurate measures of genetic diversity among different species of termites.

Key words: Termite diversity, Odontotermes, Trinervitermes, inter simple sequence repeat (ISSR), 28s rDNA, unweighted pair group method with arithmetic mean (UPGMA), multiple sequence analysis.

Aanen DK, Eggleton P, Lefèvre CR, Frøslev TG, Rosendahl S, Boomsma JJ (2002). The evolution of fungus-growing termites and their mutualistic fungal symbionts. Proceed. Nat. Acad. Sci. 99(23):14887-14892.
Crossref

 

Al-Otaibi SA (2008). Genetic variability in mite-resistant honey bee using ISSR molecular markers. Arab J. Biotechnol. 11(2):241-252.

 
 

Austin JW, Szalanski AL, Scheffrahn RH, Messenger MT (2005a). Genetic variation of Reticulitermes flavipes (Isoptera: Rhinotermitidae) in North America using the mitochondrial rRNA 16S gene. Ann. Entomol. Soc. Am. 98(6):980-988.
Crossref

 
 

Austin JW, Szalanski AL, Scheffrahn RH, Messenger MT, Dronnet S, Bagne`Res AG (2005b). Genetic Evidence for the Synonymy of Two Reticulitermes Species: Reticulitermes flavipes and Reticulitermes santonensis. Ann. Entomol. Soc. Am. 98(3):395-401.
Crossref

 
 

Bechly G (2007). Isoptera: termites. In (D.M. Martill, G. Bechly, and R.F. Loveridge editors), The Crato fossil beds of Brazil:window into an ancient world. New York: Cambridge University PRESS, 16:249-262.

 
 

Berezovskaia OP, OIu M, Sidorenko AP (2002). Intra- and interspecies differences in the ISSR-PCR patterns of bumble bee (Hymenoptera: Bombinae). Tsitol Genet. 36(3):28-35.

 
 

Bornet B, Muller C, Paulus F, Branchard M (2002). Highly informative nature of inter simple sequence repeat (ISSR) sequences amplified using tri- and tetra-nucleotide primers from DNA of cauliflower (Brassica oleracea var. botrytis L.). Genome 45:890-896.
Crossref

 
 

Carr DB, Young CJ, Aster RC, Zhang X (1999). Cluster analysis for CTBT Seismic Event Monitoring. Retrieved from 

View.

 
 

Chakravorty S, Helb D, Burday M, Connell N, Alland DA (2007). Detailed analysis of 16S ribosomal RNA gene segments for the diagnosis of pathogenic bacteria. J. Microbiol. Methods 69(2):330-339.
Crossref

 
 

Chauhan T, Rajiv K (2010). Molecular markers and their applications in fisheries and aquaculture. Adv. Biosci. Biotechnol. 1:281-291.
Crossref

 
 

Chhotani OB (1997). The Fauna of India and Adjacent Countries: Isoptera (Termites) (Volume-II Family Termopsidae). Zoological Survey of India.

 
 

Dusinsky R, Kúdela M, Stloukalova V, Jedlicka L (2006). Use of inter-simple sequence repeat (ISSR) markers for discrimination between and within species of blackflies (Diptera:Simuliidae). Biologia, Bratislav 61(3):299-304.
Crossref

 
 

Eggleton P (2001). Termites and trees:a review of recent advances in termite phylogenetics. Insect Soc., Springer 42:187-193.

 
 

Eggleton P (2010). An Introduction to Termites: Biology, Taxonomy and Functional Morphology. (Biology of Termites: A Modern Synthesis 1st ed. Editors Bignell D. E., Roisin Y. and Lo N.). Dordrecht:Springer., pp. 1-26.
Crossref

 
 

Fang DQ, Roose ML (19970. Identification of closely related citrus cultivars with inter-simple sequence repeat markers. Theor. Appl. Genet. 95:408-417.
Crossref

 
 

Garriga M, Parra PA, Caligari PDS, Retamales JB, Carrasco BA, Lobos GA, Garci’a-Gonza’les R (2013). Application of inter-simple sequence repeats relative to simple sequence repeats as a molecular marker system for indexing blueberry cultivars. Can. J. Plant Sci. 93:913-921.
Crossref

 
 

Godwin ID, Aitken EA, Smith LW (1997). Application of inter simple sequence (ISSR) markers to plant genetics. Electrophoresis 18:1524-1528.
Crossref

 
 

Hassouna N, Michot B, Bachellerie JP (1984). The complete nucleotide sequence of moose 28S rRNA gene. Implications for the process of size increase of the large snbnnit rRNA in higher eukaryotes. Nucleic Acids Res. 12(8):3563-3583.
Crossref

 
 

Husseneder C, Grace JK (2001). Similarity is Relative: Hierarchy of Genetic Similarities in the Formosan Subterranean Termite (Isoptera: Rhinotermitidae) in Hawaii. Environ. Entomol.30(2):262-266.
Crossref

 
 

Inward DJG, Vogler AP, Eggleton P (2007). A comprehensive phylogenetic analysis of termites (Isoptera) illuminates key aspects of their evolutionary biology. Mol. Phylogenet. Evol. 44:953-967.
Crossref

 
 

Jarvis KJ, Haas F, Whiting MF (2004). A phylogeny of earwigs (Insecta:Dermaptera) based on molecular and morphological evidence reconsidering the classification of Dermaptera. Syst. Entomol. 30:1-12.

 
 

Javan ZS, Rahmani F, Heidari R (2012). Assessment of genetic variation of genus Salvia by RAPD and ISSR markers. Aust. J. Crop Sci. 6(6):1068-1073.

 
 

Jenkins TM, Dean RE, Verkerk R, Forschler BT (2001). Phylogenetic analysis of two mitochondrial genes and one nuclear intron region illuminate European subterranean termite (Isoptera: Rhinotermitidae) gene ßow, taxonomy, and introduction dynamics. Mol. Phylogenet. Evol.20:286-293.
Crossref

 
 

Kambhampati S, Eggleton P (2000). Phylogenetics and taxonomy, Termites: Evolution, Sociality, Symbioses, Ecology. (T Abe, D E Bignell and M Higashi eds.) Kluwer Academic Publishing Dordrecht., pp. 1-23.

 
 

Korman AK, Pashley DP, Haverty MI, La Fage JP (1991). Allozyme relationship among cuticular hydrocarbon phenotypes of Zootermopsis species (Isoptera: Termopsidae). Ann. Entomol. Soc. Am. 84(1):1-9.
Crossref

 
 

Krishna K, Grimaldi DA, Krishna V, Engel MS (2013). Treatise on the Isoptera of the world. Bull. Am. Museum Nat. Hist. 377(1-7):1-2704.

 
 

Kumar S, Gadagkar SR (2001). Disparity Index: A simple statistic to measure and test the homogeneity of substitution patterns between molecular sequences. Genetics 158:1321-1327.

 
 

Lander ES, Botstein D (1989). Mapping Mendelian Factors Underlying Quantitative Traits Using RFLP Linkage Maps. Genetics 121:185-199.

 
 

Liu B, Wendel JF (2001). Intersimple sequence repeat (ISSR) polymorphisms as a genetic marker system in cotton. Mol. Ecol. Notes 1:205-208.
Crossref

 
 

Long Y, Xiang H, Xie L, Yan X, Fan M, Wang Q (2009). Intra-and Interspecific Analysis of Genetic Diversity and Phylogeny of Termites (Isoptera) in East China Detected by ISSR and COII Markers. Sociobiology 53(2B):411-430.

 
 

Magurran AE (2013). Chapter one: Introduction: measurement of (biological) diversity. In Measuring Biological Diversity. Hoboken, NJ:Wiley-Blackwell. Pp. 11-14.

 
 

Marini M, Mantovani B (2002). Molecular relationships among european samples of Reticulitermes (Isoptera, Rhinotermitidae). Mol. Phylogenet. Evol. 22:454-459.
Crossref

 
 

Moulin MM, Rodrigues R, Gonçalves LS A, Sudré C P, Pereira M G (2012). A comparison of RAPD and ISSR markers reveals genetic diversity among sweet potato landraces (Ipomoea batatas (L.) Lam.). Acta Scientiarum 34(2):139-147.
Crossref

 
 

Ohkuma M, Yuzawa H, Amornsak W, Sornnuwat Y, Takematsu Y, Yamada A, Vongkaluang C, Sarnthoy O, Kirtibutr N, Noparatnaraporn N, Kudo T, Inoue T (2004). Molecular phylogeny of Asian termites (Isoptera) of the families Termitidae and Rhinotermitidae based on mitochondrial COII sequences. Mol. Phylogenet. 31:701-710.
Crossref

 
 

Okpul T, Mace ES, Godwin ID, Singh D, Wagih ME (2005). Evaluation of variability among breeding lines and cultivars of taro (Colocasia esculenta) in Papua New Guinea using ISSR fingerprinting and agro-morphological characterization. PGR Newsletter 143:8-16.

 
 

Ouvrard D, Campbell BC, Bourgoin T, Chan KL (2000). 18S rRNA Secondary structure and phylogenetic position of Peloridiidae (Insecta, Hemiptera). Mol. Phylogenet. Evol. 16:403-417.
Crossref

 
 

Pei AY, Oberdorf WE, Nossa CW, Agarwal A, Chokshi P, Gerz AE, Jin Z., Lee P, Yang L, Poles M, Brown SM, Sotero S, De Santis T, Brodie E, Nelson K, Pei Z (2010). Diversity of 16S rRNA Genes within Individual Prokaryotic Genomes. Appl. Environ. Microbiol. 76(12):3886-3897.
Crossref

 
 

Pranesh MK, Harini BP (2014). Comparative morphological divergence among few common Indian species of the family Termitidae:Isoptera. Int. J. Adv. Res. 2(4):523-531.

 
 

Reuter JS, Mathews DH (2010). RNA structure: software for RNA secondary structure prediction and analysis. BMC Bioinform. 11:129.
Crossref

 
 

Roonwal ML, Chhotani (1989). The Fauna of India and Adjacent Countries: Isoptera (Termites) (Volume-1 Introduction and Families of Termopsidae, Hodotermitidae. Saitou N, Nei M (1987). The neighbor-joining method: A new method for reconstructing phylogenetic trees. Mol. Biol. Evol. 4:406-425.

 
 

Salhi-Hannachi A, Chatti K, Mars M, Marrakchi M, Trifi M (2005). Comparative analysis of genetic diversity in two Tunisian collections of fig cultivars base on random amplified polymorphic DNA and inter simple sequence repeats fingerprints. Genet. Res. Crop Evol. 52:563-573.
Crossref

 
 

Salhi-Hannachi A, Trifi M, Zehdi S, Hedhfi J, Mars M, Rhouma A, Minter M (2004). Simple sequence repeat fingerprints to access genetic diversity in Tunisian fig (Ficus carica L.) germplasm. Genet. Res. Crop Evol. 51:269-275.
Crossref

 
 

Sambrook J, Russell DW (2001). Molecular Cloning: A laboratory manual 3rd ed. Cold Spring Harbor Laboratory Press, New York.

 
 

Sanchez MP, Davila JA, Loarce Y, Ferrer E (1996). Simple sequence repeat primers used in polymerase chain reaction amplifications to study genetic diversity in barley. Genome 39:112-117.
Crossref

 
 

Scharf ME, Wu-Scharf D, Zhou X, Pittendrigh BR, Bennett GW (2005). Gene expression profiles among immature and adult reproductive castes of the termite Reticulitermes flavipes. The Royal Entomological Society, Insect Molecular Biology 14(1):31-44.
Crossref

 
 

Semagn K, Bjørnstad Å, Ndjiondjop MN (2006). An overview of molecular marker methods for plants. Afr. J. Biotechnol. 5(25):2540-2568.

 
 

Shanmugavel S, Paulchamy R, Velayutham V, Sundaram J (2015). Genetic diversity based on 28S rDNA sequences among populations of Culex quinquefasciatus collected at different locations in Tamil Nadu, India. Trop. Biomed. 32:434-443.

 
 

Sheppard WS, Smith DR (2000). Identification of African-derived bees in the Americas:A survey of methods. Ann. Entomol. Soc. Am. 93:159-176.
Crossref

 
 

Sorkheh K, Dehkordi MK, Ercisli S, Hegedus A, Halász J (2017). Comparison of traditional and new generation DNA markers declares high genetic diversity and differentiated population structure of wild almond species. Scientific Reports 7, Article number: 5966.
Crossref

 
 

Tamura K, Nei M, Kumar S (2004). Prospects for inferring very large phylogenies by using the neighbor-joining method. Proceed. Natl. Acad. Sci. USA 101:11030-11035.
Crossref

 
 

Torre MP, García M, Heinz R, Escandón A (2012). Analysis of genetic variability by ISSR markers in Calibrachoa caesia. Electronic Journal of Biotechnology 15(5):8-8.

 
 

Wang C, Zhou X, Li S, Schwinghammer M, Scharf ME, Buczkowski G, Bennett GW (2009). Survey and Identification of Termites (Isoptera: Rhinotermitidae) in Indiana. Ann. Entomol. Soc. Am. 102(6):1029-1036.
Crossref

 
 

Whiting MF (2002). Mecoptera is paraphyletic:multiple genes and phylogeny of Mecoptera and Siphonaptera. Zool. Scr. 31:93-104.
Crossref

 
 

Yeap BK, Duga FM, Othman AS, Lee CY (2010). Genetic Relationship Between Coptotermes heimi and Coptotermes gestroi (Isoptera: Rhinotermitidae). Sociobiology 56(2):291-312.

 
 

Yu S, Wang Y, Rédei D, Xie Q, Bu W (2013). Secondary structure models of 18S and 28S rRNAs of the true bugs based on complete rDNA sequences of Eurydema maracandica Oshanin, 1871 (Heteroptera, Pentatomidae). ZooKeys 319:363-377.
Crossref

 

 


APA Harini, B. P., & Pranesh, M. K. (2018). Intra and inter specific genetic divergence of termites species based on ISSR markers and 28S rDNA sequences. International Journal of Biodiversity and Conservation, 10(2), 52-91.
Chicago Ballagere Puttaraju Harini and Muduvadi Krishnamurthy Pranesh. "Intra and inter specific genetic divergence of termites species based on ISSR markers and 28S rDNA sequences." International Journal of Biodiversity and Conservation 10, no. 2 (2018): 52-91.
MLA Ballagere Puttaraju Harini and Muduvadi Krishnamurthy Pranesh. "Intra and inter specific genetic divergence of termites species based on ISSR markers and 28S rDNA sequences." International Journal of Biodiversity and Conservation 10.2 (2018): 52-91.
   
DOI https://doi.org/10.5897/IJBC2017.1146
URL http://www.academicjournals.org/journal/IJBC/article-abstract/FAFA5E755531

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