African Journal of Pharmacy and Pharmacology
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Article Number - 4B78EBE55367


Vol.9(36), pp. 900-914 , September 2015
DOI: 10.5897/AJPP2015.4385
ISSN: 1996-0816



Review

Toxicity of food colours and additives: A review



Olusegun E. Thomas
  • Olusegun E. Thomas
  • Department of Pharmaceutical Chemistry, Faculty of Pharmacy,University of Ibadan, Orita UI, Ibadan, Nigeria.
  • Google Scholar
Olajire A. Adegoke*
  • Olajire A. Adegoke*
  • Department of Pharmaceutical Chemistry, Faculty of Pharmacy,University of Ibadan, Orita UI, Ibadan, Nigeria.
  • Google Scholar







 Received: 18 June 2015  Accepted: 10 August 2015  Published: 29 September 2015

Copyright © 2015 Author(s) retain the copyright of this article.
This article is published under the terms of the Creative Commons Attribution License 4.0


Majority of consumer goods are required to be presented with good aesthetics in order to improve acceptability in terms of colours and in some instances taste. When related to food, beverages and drug products, additives are usually added to mask un-inviting colours, obscure offensive odours and increase taste. Food additives therefore include colourants, sweeteners, preservatives and anti-caking agents. Admissible daily intake limits are often recommended for these additives. Being food products, the amount consumed over time may be subject to individual preferences and thus negating the desire to regulate and control the amount consumed cumulatively. There have been several concerns about the safety of food additives and several batteries of tests, and reports are available in literature. This review attempted to give an update on reports that have surfaced in literature over recent past on the use and safety of food colours and other additives. Some safety concerns have been related to three determinations; cytotoxicity, genotoxicity and induction or potential of inducing mutagenicity. In order to accomplish these targeted evaluations, several tests have been prescribed by International conference on harmonization (ICH), organization for economic co-operation and development (OECD) and European food safety authority (EFSA). It is observed that no single test can give a full proof of safety of these food colours and additives, hence minimal tests are recommended to be carried out in order to guarantee safety of these products. Survey of literature, revealed that once some approved additives or colours become a subject of safety concerns, comprehensive evaluations are carried out by researchers and these have often led to the de-classification of some hitherto reported agents as being non-genotoxic or non-carcinogenic. The declassifications of some food colors and additives as human carcinogens are regularly done following the comprehensive evaluation of results of mutagenicity and genotoxicity tests in vitro and some in vivo tests in mammalian tissues and whole animals. However, such declassifications are often done with caution and the implication is that regular and more comprehensive tests must be carried out. In addition, the requirements of testing for chronic exposures to this and other agents must be emphasized to prevent occurrence of subtle yet terrible side effects resulting from consuming sub-toxic doses of the additives over time.

 

Key words: Food colour, food additive, cytotoxicity, genotoxicity, mutagenicity, safety concerns.

Abdel-Aziz AH, Shouman SA, Attia AS, Saad SF (1997). A study on the reproductive toxicity of erythrosine in male mice. Pharmacol. Res. 35(5):457-462.
Crossref
 
Abramsson-Zetterberg L, Ilbäck NG (2013). The synthetic food colouring agent Allura Red AC (E129) is not genotoxic in a flow cytometry-based micronucleus assay in vivo. Food Chem. Toxicol. 59:86-89.
Crossref
 
Adegoke OA, Ghosh M, Mukherjee A (2012b). Spectrophotometric and thermodynamic studies of the interactions of 4-carboxyl-2,6-dinitrophenylazohydroxynaphthalenes with bovine serum albumin. Spectrochim. Acta A Mol. Biomol. Spectrosc. 96:1038-1046.
Crossref
 
Adegoke OA, Kyu JK, Mukherjee A (2012a). In vitro genotoxicity evaluation of 4-carboxyl-2,6-dinitrophenylazohydroxynaphthalenes using human lymphocytes. Food Chem. Toxicol. 50(3-4):936-941.
Crossref
 
Auletta AE, Kuzava JM, Parmar AS (1977). Lack of mutagenic activity of a series of food dyes for Salmonella typhimurium. Mutat. Res. 56:203.
Crossref
 
Bandyopadhyay A, Ghoshal S, Mukherjee A (2008). Genotoxicity testing of low-calorie sweeteners: aspartame, acesulfame-K, and saccharin. Drug Chem. Toxicol. 31(4):447-457.
Crossref
 
Benz DR, Matthews EJ, Kruhlak NL, Frid AA, Minnier BL, Contrera JF (2007). Toxicological and clinical computational analysis by the Informatics and Computational Safety Analysis Staff of the US FDA/CDER. AATEX 14, Special Issue, 463-467, Proc. 6th World Congress on Alternatives & Animal Use in the Life Sciences, August 21-25, 2007, Tokyo, Japan. Available at: http://www.asas.or.jp/jsaae_old/zasshi/WC6_PC/paper463.pdf
 
Bonin AM, Baker RS (1980). Mutagenicity testing of some approved food additives with Salmonella microsome assay. Food Technol. Aust. 32(12):608-611.
 
Bonser GM, Clayson DB, Jull JW (1956). The induction of tumours of the subcutaneous tissues, liver and intestine in the mouse by certain dyestuffs and their intermediates. Br. J. Cancer 10(4):653-667.
Crossref
 
Borzelleca JF, Capen CC, Hallagan JB (1987). Lifetime toxicity/carcinogenicity study of FD & C Red No. 3 (erythrosine) in rats. Food Chem. Toxicol. 25(10):723-733.
Crossref
 
Borzelleca JF, Depukat K, Hallagan JB (1990). Lifetime toxicity/carcinogenicity studies of FD & C Blue No. 1 (brilliant blue FCF) in rats and mice. Food Chem. Toxicol, 28(4):221-234.
Crossref
 
Borzelleca JF, Hallagan JB (1987). Lifetime toxicity/carcinogenicity study of FD & C Red No. 3 (erythrosine) in mice. Food Chem. Toxicol. 25(10):735-737.
Crossref
 
Bowen DE, Whitwell JH, Lillford L, HendersonD, Kidd D, Mc Garry S, Kirkland DJ (2011). Evaluation of a multi-endpoint assay in rats, combining the bone-marrow micronucleus test, the Comet assay and the flow-cytometric peripheral blood micronucleus test. Mutat. Res. 722:7-19.
Crossref
 
Brait M, Sidransky D (2011). Cancer epigenetics: above and beyond. Toxicol. Mech. Methods 21(4):275-288.
Crossref
 
Brambilla G, Martelli A (2009). Update on genotoxicity and carcinogenicity testing of 472 marketed pharmaceuticals. Mutat. Res. 681(2-3):209-229.
Crossref
 
Brown JP, Roehm GW, Brown RJ (1978). Mutagenicity testing of certified food colors and related azo, xanthene and triphenylmethane dyes with the Salmonella/microsome system. Mutat. Res. 56(3):249-271.
Crossref
 
Burlinson B, Tice RR, Speit G, Agurell E, Brendler-Schwaab SY, Collins AR, Hartmann A (2007). Fourth International Workgroup on Genotoxicity testing:Results of the in vivo Comet assay workgroup. Mutat. Res. 627:31-35.
Crossref
 
Capitan-Vallvey LF, Valencia MC, Nicolas EA, Garcia- Jimenez JF (2006). Resolution of an intense sweetener mixture by use of a flow injection sensor with on-line solid-phase extraction. Application to saccharin and aspartame in sweets and drinks. Anal. Bioanal. Chem. 385:385-391.
Crossref
 
Chequer F, Dorta D, Palma de Oliveira D (2011). Azo Dyes and their Metabolites: Does the Discharge of the Azo Dye into Water Bodies Represent Human and Ecological Risks? Advances in Treating Textile Effluent (P. P. Hauser Ed.) Croatia: InTech.
 
Chequer FM, Venancio VP, Bianchi ML, Antunes LM (2012). Genotoxic and mutagenic effects of erythrosine B, a xanthene food dye, on HepG2 cells. Food Chem. Toxicol. 50(10):3447-3451.
Crossref
 
Chung K, Stevens S, Cerniglia C (1992). The reduction of azo dyes by the intestinal microflora. Crit. Rev. Microbiol. 18(3):175-190.
Crossref
 
Chung KT, Fulk G, Andrews A (1981). Mutagenicity testing of some commonly used dyes. Appl. Environ. Microbiol. 42(4):641-648.
 
Cohen SM, Anderson TA, de Oliveira LM, Arnold LL (1998). Tumorigenicity of Sodium Ascorbate in Male Rats. Cancer Res. 58(12):2557-2561.
 
Collins TF, Black TN, Brown LH, Bulhack P (1990). Study of the teratogenic potential of FD & C Yellow No. 5 when given by gavage to rats. Food Chem. Toxicol. 28(12):821-827.
Crossref
 
Collins TF, Black TN, O'Donnell MWJ, Bulhack P (1992). Study of the teratogenic potential of FD & C yellow No. 5 when given in drinking-water. Food Chem. Toxicol. 30(4):263-268.
Crossref
 
Collins TF, Black TN, O'Donnell MWJ, Shackelford ME, Bulhack P (1993a). Teratogenic potential of FD & C red no. 3 when given in drinking water. Food Chem. Toxicol. 31(3):161-167.
Crossref
 
Collins TF, Black TN, Ruggles DI (1993b). Teratogenic potential of FD&C Red No. 3 when given by gavage. Toxicol. Ind. Health 9(4):605-616.
 
Combes RD, Haveland-Smith RB (1982). A review of the genotoxicity of food, drug and cosmetic colours and other azo, triphenylmethane and xanthene dyes. Mutat. Res. 98(2):101-243.
Crossref
 
Contrera JF, Kruhlak NL, Matthews EJ, Benz RD (2007). Comparison of MC4PC and MDL-QSAR rodent carcinogenicity predictions and the enhancement of predictive performance by combining QSAR models. Regul. Toxicol. Pharmacol. 49(3):172-182.
Crossref
 
Das A, Mukherjee A (2004). Genotoxicity testing of food colours amaranth and tartrazine. Int. J. Hum. Gen. 4:277-280.
 
Dybing E, Holme JA, Perry GW, Søderlund EJ, Dahlin DC, Nelson SD (1984). Genotoxicity studies with paracetamol. Mutat. Res.138(1):21-32.
Crossref
 
EFSA (2009a). EFSA Panel on Food Additives and Nutrient Sources added to Food; Scientific Opinion on the re-evaluation of Tartrazine (E 102) as a food additive. EFSA 7:1331-1383.
 
EFSA (2009b). EFSA Panel on Food Additives and Nutrient Sources added to Food: Scientific Opinion on the re-evaluation of Allura Red AC (E 129) as a food additive. EFSA 7(11):1327-1366.
 
EFSA (2009c). EFSA Panel on Food Additives and Nutrient Sources added to Food (ANS): Scientific Opinion on the re-evaluation of Sunset Yellow FCF (E 110) as a food additive. EFSA 7(11):1330-1374.
 
EFSA (2010). EFSA Panel on Food Additives and Nutrient Sources added to Food:Scientific Opinion on the re-evaluation of Brilliant Blue FCF (E 133) as a food additive. EFSA 8(11):1853-1889.
 
EFSA (2011). EFSA Panel on Food Additives and Nutrient Sources added to Food; Scientific Opinion on the re-evaluation of Erythrosine (E 127) as a food additive. EFSA 9(1):1854-1900.
 
EFSA (2012). Scientific Opinion on the safety and efficacy of Allura Red AC (E 129) in feed for cats and dogs. EFSA 10(5):2675-2689.
 
EFSA (2013). EFSA Panel on Food Additives and Nutrient Sources added to Food; Scientific Opinion on the re-evaluation of aspartame (E 951) as a food additive. EFSA 11(12):3496-3759.
 
Elhkim MO, Heraud F, Bemrah N, Gauchard F, Lorino T, Lambre C, Poul JM (2007). New considerations regarding the risk assessment on Tartrazine An update toxicological assessment, intolerance reactions and maximum theoretical daily intake in France. Regul. Toxicol. Pharmacol. 47(3):308-316.
Crossref
 
EU (2015). Current EU approved additives and their E Numbers. Available at: https://www.food.gov.uk/science/additives/enumberlist
 
FDA (1993). 1993 Draft Redbook II: Toxicological Principles for the Safety Assessment of Direct Food Additives and Color Additives Used in Food. Redbook II Draft Guidance. Available at: http://www.fda.gov/Food/GuidanceRegulation/GuidanceDocumentsRegulatoryInformation/IngredientsAdditivesGRASPackaging/ucm078717.htm
 
FDA (2015). Summary of Color Additives for Use in the United States in Foods, Drugs, Cosmetics, and Medical Devices. Available at: http://www.fda.gov/ForIndustry/ColorAdditives/ColorAdditiveInventories/ucm115641.htm
 
Fraumeni Jr J, Miller R (1972). Drug-induced cancer. J. Natl. Cancer Inst. 48(5):1267.
 
Gaunt IF, Mason PL, Grasso P, Kiss IS (1974). Long-term toxicity of Sunset Yellow FCF in mice. Food Cosmet. Toxicol. 12(1):1-9.
Crossref
 
Golka K, Kopps S, Myslak Z (2004). Carcinogenicity of azo colorants: influence of solubility and bioavailability. Toxicol. lett. 151(1):203-210.
Crossref
 
Guengerich FP (2001). Forging the links between metabolism and carcinogenesis. Mutat. Res. 488:195-209.
Crossref
 
Güngörmüş C, Kılıç A (2012). The Safety Assessment of Food Additives by Reproductive and Developmental Toxicity Studies. Food Additive, Hacettepe University/Department of Biology, Ankara Turkey. Available at: http://www.intechopen.com/books/food-additive/the-safety-assessment-of-food-additives-by-reproductive-and-developmental-toxicity-studies
 
Hansen WH, Zwickey RE, Brouwer JB, Fitzhugh OG (1973). Long-term toxicity studies of erythrosine. I. Effects in rats and dogs. Food Cosmet. Toxicol. 11(4):527-534.
Crossref
 
Hasegawa M, Nishi Y, Ohkawa Y, Inui N (1984). Effects of sorbic acid and its salts on chromosome aberrations, sister chromatid exchanges and gene mutations in cultured Chinese hamster cells. Food Chem. Toxicol. 22(7):501-507.
Crossref
 
Haveland-Smith RB, Combes RD (1980). Screening of food dyes for genotoxic activity. Food Cosmet. Toxicol. 18(3):215-221.
Crossref
 
Heddle JA, Cimino MC, Hayashi M, Romagna F, Shelby MD, Tucker JD, Mac Gregor JT (1991). Micronuclei as an index of cytogenetic damage: past, present, and future. Environ. Mol. Mutagen. 18(4):277-291.
Crossref
 
Heddle JA, Dean S, Nohmi T, Boerrigter M, Casciano D, Douglas GR, Martus H (2000). In vivo transgenic mutation assays. Environ. Mol. Mutagen. 35(3):253-259.
Crossref
 
Heddle JA, Hite M, Kirkhart B, Mavournin K, MacGregor J, Newell GW, Salamone MF (1983). The induction of micronuclei as a measure of genotoxicity: a report of the US Environmental Protection Agency Gene-Tox Program. Mutat. Res.123(1):61-118.
Crossref
 
Hendry LB, Mahesh VB, Bransome Jr ED, Ewing DE (2007). Small molecule intercalation with double stranded DNA- Implications for normal gene regulation and for predicting the biological efficacy and genotoxicity of drugs and other chemic. Mutat. Res. 623:53-71.
Crossref
Himri I, Bellahcen S, Souna F, BelmakkiF, Aziz M, Bnouham M, Saalaoui E (2011). A 90-day oral toxicity study of tartrazine, a synthetic food dye, in wistar rats. Int. J. Pharm. Pharm. Sci. 3(Suppl 3):159-169.
 
ICH (1997). Genotoxicity: A Standard Battery for Genotoxicity Testing of Pharmaceuticals S2B Step 4 version International Conference on Harmonization (ICH) of technical requirements for registration of Pharmaceuticals for human use.
 
Ishidate Jr M, Odashima S (1977). Chromosome tests with 134 compounds on Chinese hamster cells in vitro--a screening for chemical carcinogens. Mutat. Res. 48(3-4):337-353.
Crossref
 
Ishidate Jr M, Sofuni T, Yoshikawa K, Hayashi M, Nohmi T, Sawada M, Matsuoka A (1984). Primary mutagenicity screening of food additives currently used in Japan. Food Chem. Toxicol. 22(8):623-636.
Crossref
 
Jabeen HS, ur Rahman S, Mahmood S, Anwer S (2013). Genotoxicity assessment of amaranth and allura red using Saccharomyces cerevisiae. Bull. Environ. Contam. Toxicol. 90(1):22-26.
Crossref
 
JECFA (1982). 26th Report of the Joint FAO/WHO Expert Committee on Food Additives. Toxicological evaluation of certain food additives. WHO Food Additives Series, No. 17.
 
JECFA (1986). Toxicological evaluation of certain food additives and contaminants. 30th Report. WHO Food Additives Series.
 
Jeffrey AM, Williams GM (2000). Lack of DNA-damaging activity of five non-nutritive sweeteners in the rat hepatocyte/DNA repair assay. Food Chem. Toxicol. 38(4):335-338.
Crossref
 
Jung R, Cojocel C, Muller W, Bottger D, Luck E (1992). Evaluation of the genotoxic potential of sorbic acid and potassium sorbate. Food Chem. Toxicol. 30(1):1-7.
Crossref
 
Kamel MM, El-lethey HS (2011). The potential health hazard of tartrazine and levels of hyperactivity, anxiety-like symptoms, depression and anti-social behaviour in rats. J. Am. Sci. 7(6):1211-1218.
 
Kawaguchi S, Nakamura T, Yamamoto A, Honda G, Sasaki Y (2010). Is the comet assay a sensitive procedure for detecting genotoxicity? J. Nucleic Acids 2010:541050.
Crossref
 
Kawasaki H, Abe H (2012). Epigenetics in cancer and inflammation. Personal. Med. Universe 1:7-12.
 
Keshavarz MH, Gharagheizi F, Shokrolahi A, Zakinejad S (2012). Accurate prediction of the toxicity of benzoic acid compounds in mice via oral without using any computer codes. J. Hazard. Mater. 237–238:79-101.
Crossref
 
Kirkland K, Speit G (2008). Evaluation of the ability of a battery of three in vitro genotoxicity tests to discriminate rodent carcinogens and non-carcinogens III. Appropriate follow-up testing in vivo. Mutat. Res. 654: 114-132.
Crossref
 
Kirsch-Volders M, Decordier I, Elhajouji A, Plas G, Aardema M, Fenech M (2011). In vitro genotoxicity testing using the micronucleus assay in cell lines, human lymphocytes and 3D human skin models. Mutagenesis 26(1):177-184.
Crossref
 
Kristensen LS, Nielsen HM, Hansen LL (2009). cancer and epigenetics. Eur. J. Pharmacol. 625:131-142.
Crossref
 
Kumaravel TS, Jha AN (2006). Reliable Comet assay measurements for detecting DNA damage induced by ionising radiation and chemicals. Mutat Res. 605(1):7-16.
Crossref
 
Lakdawalla AA, Netrawali MS (1988a). Mutagenicity, comutagenicity, and antimutagenicity of erythrosine (FD and C red 3), a food dye, in the Ames/Salmonella assay. Mutat. Res. 204(2):131-139.
Crossref
 
Lakdawalla AA, Netrawali MS (1988b). Erythrosine, a permitted food dye, is mutagenic in the Bacillus subtilis multigene sporulation assay. Mutat. Res. 206(2):171-176.
Crossref
 
Lambert IB, Singer TM, Boucher SE, Douglas GR (2005). Detailed review of transgenic rodent mutation assays. Mutat. Res. 590(1):1-280.
Crossref
 
Leconinte P, Lesca P (1978). Absence of activity of amaranth (FD & C red no. 2) in the Salmonella/microsome mutagenicity test. Food Cosmet. Toxicol. 16(1): 89-90.
Crossref
 
Li Y, Wei H, Liu R (2014). A probe to study the toxic interaction of tartrazine with bovine hemoglobin at the molecular level. Luminescence 29(2):195-200.
Crossref
 
Lovell D, Pa OT (2008). Statistical issues in the use of the comet assay. Mutagenesis 23(3): 171-182.
Crossref
 
Maekawa A, MatsuokaC, Onodera H, TanigawaH, Furuta K, Kanno J, Ogiu T (1987). Lack of carcinogenicity of tartrazine (FD & C Yellow No. 5) in the F344 rat. Food Chem. Toxicol. 25(12): 891-896.
Crossref
 
Magnuson BA, Burdock GA, Doull J, Kroes RM, Marsh GM, Pariza MW, Williams GM (2007). Aspartame: a safety evaluation based on current use levels, regulations, and toxicological and epidemiological studies. CRC Crit. Rev. Toxicol 37(8):629-727.
Crossref
 
Mahadevan B, Snyder RD, Waters MD, Benz RD, Kemper RA, Tice RR (2011). Genetic toxicology in the 21st century: Reflections and future directions. Environ. Mol. Mutagen. 52(5): 339-354.
Crossref
 
Mamur S, Yüzbaşıoğlu D, Ünal F, Yılmaz S (2010). Does potassium sorbate induce genotoxic or mutagenic effects in lymphocytes? Toxicol. In vitro 24(3):790-794.
Crossref
 
Mannell WA, Grice HC (1964). Chronic toxicity of brilliant blue FCF, blue VRS, and green S in rats. J. Pharm. Pharmacol. 16(1):56-59.
Crossref
 
Mannell WA, Grice HC, Allmark MG (1962). Chronic Toxicity Studies on Food Colours: V. Observations on the Toxicity of Brilliant Blue FCF, Guinea Green B and Benzyl Violet 4B in Rats. J. Pharm. Pharmacol. 14(1):378-384.
Crossref
 
Matula TI, Downie RH (1984). Genetic toxicity of erythrosine in yeast. Mutat. Res. 138(2-3):153-156.
Crossref
 
Mehedi N, Ainad-Tabet S, Mokrane N, Addou S, Zaoui C, Kheroua O, Saidi D (2009). Reproductive Toxicology of Tartrazine (FD and C Yellow No. 5) in Swiss Albino Mice. Am. J. Pharmacol. Toxicol. 4(4):130-135.
Crossref
 
Mizutani T (2009). Toxicity of Xanthene Food Dyes by Inhibition of Human Drug-Metabolizing Enzymes in a Noncompetitive Manner. J. Environ. Public Health p 9.
Crossref
 
Moller P, Wallin H (2000). Genotoxic hazards of azo pigments and other colorants related to 1-phenylazo-2-hydroxynaphthalene. Mutat Res. 462:13-30.
Crossref
 
Mpountoukas P, Pantazaki A, Kostareli E, Christodoulou P, Kareli D, Poliliou S, Lialiaris T (2010). Cytogenetic evaluation and DNA interaction studies of the food colorants amaranth, erythrosine and tartrazine. Food Chem. Toxicol. 48(10):2934-2944.
Crossref
 
Mukherjee A, Chakrabarti J (1997). In vivo cytogenetic studies on mice exposed to acesulfame-K--a non-nutritive sweetener. Food Chem. Toxicol. 35(12):1177-1179.
Crossref
 
Mukhopadhyay M, Mukherjee A, Chakrabarti J (2000). In vivo cytogenetic studies on blends of aspartame and acesulfame-K. Food Chem. Toxicol. 38(1):75-77.
Crossref
 
Münzner R, Guigas C, Renner HW (1990). Re-examination of potassium sorbate and sodium sorbate for possible genotoxic potential. Food Chem. Toxicol. 28(6):397-401.
Crossref
 
Nakamura SI, Oda Y, Shimada T, Oki I, Sugimoto K (1987). SOS-inducing activity of chemical carcinogens and mutagens in Salmonella typhimurium TA1535/pSK1002: examination with 151 chemicals. Mutat. Res. 192(4):239-246.
Crossref
 
Njagi GD, Gopalan HN (1982). Cytogenetic effects of the food preservatives--sodium benzoate and sodium sulphite on Vicia faba root meristems. Mutat. Res. 102(3):213-219.
Crossref
 
OECD (1997). Test No. 471: Bacterial Reverse Mutation Test, OECD Guidelines for the Testing of Chemicals. Adopted 21/07/97. Available at: www.oecd.org/chemicalsafety/risk-assessment/1948418.pdf
 
OECDS/SIDS Initial Assessment Report (2001) Benzoates: Benzoic acid, Sodium Benzoate, Potassium Benzoate, Benzyl alcohol. Available at: www.inchem.org/documents/sids/sids/BENZOATES.pdf
 
Okada E, Fujiishi Y, Narumi K, Yasutake N, Ohyama W (2013). A four-day oral treatment regimen for simultaneous micronucleus analyses in the glandular stomach, colon, and bone marrow of rats. Mutat. Res. 758(1):87-94.
Crossref
 
Olive PL, Banáth JP (2006). The comet assay: a method to measure DNA damage in individual cells. Nat. Protoc. 1(1):23-29.
Crossref
 
Onyemaobi OI, Williams GO, Adekoya KO (2012). Cytogenetic Effects of two food preservatives, sodium metabisulphite and sodium benzoate on the root tips of Allium cepa Linn. Ife J. Sci. 14(1):155.
 
Poul M, Jarry G, Elhkim MO, Poul JM (2009). Lack of genotoxic effect of food dyes amaranth, sunset yellow and tartrazine and their metabolites in the gut micronucleus assay in mice. Food Chem. Toxicol. 47(2):443-448.
Crossref
 
Rafii F, Cerniglia C (1995). Reduction of azo dyes and nitroaromatic compounds by bacterial enzymes from the human intestinal tract. Environ. Health Perspect. 103(Suppl 5):17-19.
Crossref
 
Rall D (1975). Candidate drugs for carcinogenic property studies: procedures. J. Clin. Pharmacol. 15(1):1-4.
Crossref
 
Rogers CG, Boyes BG, Matula TI, Heroux-Metcalf C, Clayson DB (1988). A multiple end-point approach to evaluation of cytotoxicity and genotoxicity of erythrosine (FD and C Red No. 3) in a V79 hepatocyte-mediated mutation assay. Mutat. Res. 205(1-4):415-423.
Crossref
 
Sarasin A (2003). An overview of the mechanisms of mutagenesis and carcinogenesis. Mutat. Res. 544(2):99-106.
Crossref
 
Sasaki YF, Sekihash K, lzumiyama F, Nishidate E,Saga A,Ishida K, Tsuda S (2000). The Comet Assay with Multiple Mouse Organs: Comparison of Comet Assay Results andCarcinogenicity with 208 Chemicals selected fromthe IARC Monographs and U.S. NTPCarcinogenicity Database. Crit. Rev. Toxicol. 30(6):629-799.
Crossref
 
SCCP (2005). Scientific Committee on Consumer Products: Opinion on benzoic acid and sodium benzoate. sccp/0891/05. Available at: ec.europa.eu/health/ph_risk/committees/04_sccp/.../sccp_o_015.pdf‎
 
Schiffmann D, Schlatter J (1992). Genotoxicity and cell transformation studies with sorbates in Syrian hamster embryo fibroblasts. Food Chem. Toxicol. 30(8):669-672.
Crossref
 
Schlatter J, Wurgler F, Kranzlin R, Maier P, Holliger E, Graf U (1992). The potential genotoxicity of sorbates: effects on cell cycle in vitro in V79 cells and somatic mutations in Drosophila. Food Chem. Toxicol. 30(10):843-851.
Crossref
 
Serafimova R, Fuart-Gatnik M, Worth A (2010). Review of QSAR Models and Software for predicting carcinogenicity JRC Scientific and Technical Report. Luxembourg.
 
Shimada C, KanoK, Sasaki YF, Sato I, Tsuda S (2010). Differential colon DNA damage induced by azo food additives between rats and mice. J. Toxicol. Sci. 35(4):547-554.
Crossref
 
Sodemoto Y, Enomoto M(1980). Report of carcinogenesis bioassay of sodium benzoate in rats: absence of carcinogenicity of sodium benzoate in rats. J. Environ. Pathol. Toxicol. 4(1):87-95.
 
Speit G, Hartmann A (2006). The Comet Assay: A Sensitive Genotoxicity Test for the Detection of DNA Damage and Repair. In:
Crossref
 
Squire RA (1985). Histopathological evaluation of rat urinary bladders from the IRDC two-generation bioassay of sodium saccharin. Food Chem. Toxicol. 23(4-5):491-497.
Crossref
 
Sugimura T, Terada M, Yokota J, Hirohashi S, Wakabayashi K (1992). Multiple genetic alterations in human carcinogenesis. Environ. Health Perspect. 98:5-12.
Crossref
 
Tanaka T (2001). Reproductive and neurobehavioural toxicity study of erythrosine administered to mice in the diet. Food Chem. Toxicol. 39(5):447-454.
Crossref
 
Taylor JM, Weinberger MA, Friedman L (1980). Chronic toxicity and carcinogenicity to the urinary bladder of sodium saccharin in the in utero-exposed rat. Toxicol. Appl. Pharmacol. 54(1):57-75.
Crossref
 
Tice RR, Agurell E, Anderson D, Burlinson B, Hartmann A, Kobayashi H, Sasaki YF (2000). Single cell gel/comet assay: guidelines for in vitro and in vivo genetic toxicology testing. Environ. Mol. Mutagen. 35(3):206-221.
Crossref
 
Toth B (1984). Lack of Tumorigenicity of Sodium Benzoate in Mice. Toxicol. Sci. 4(3part1):494-496.
Crossref
 
Tsuda S, Murakami M, Matsusaka N, Kano K, Taniguchi K, Sasaki YF (2001). DNA Damage Induced by Red Food Dyes Orally Administered to Pregnant and Male Mice. Toxicol. Sci. 61(1):92-99.
Crossref
 
Vivekanandhi J, Devi CP, Jayaraman K, Raghavan L (2006). Effect of erythrosine on testicular function of mice. Toxicol. Int. 13(2):119-125.
 
Vorhees CV, Butcher RE, Brunner RL, Wootten V, Sobotka TJ (1983). A developmental toxicity and psychotoxicity evaluation of FD and C red dye# 3 (erythrosine) in rats. Arch. Toxicol. 53(4):253-264.
Crossref
 
Walker R (1990). Toxicology of sorbic acid and sorbates. Food Addit. Contam. 7(5):671-676.
Crossref
 
Weihrauch MR, Diehl V (2004). Artificial sweeteners--do they bear a carcinogenic risk? Ann. Oncol. 15(10):1460-1465.
Crossref
 
Wever J, Munzner R, Renner HW (1989). Testing of sunset yellow and orange II for genotoxicity in different laboratory animal species. Environ. Mol. Mutagen. 13(3):271-276.
Crossref
 
Whitehouse CR, Boullata J, McCauley LA (2008). The potential toxicity of artificial sweeteners. AAOHN J. 56(6):251-259; quiz 260-251.
 
Yilmaz S, Uenal F, Aksoy H, Yuezbasioglu D, Celik M (2008). Cytogenetic effects of citric acid and benzoic acid on Allium chromosomes. Fresenius Environ. Bull. 17(8 A):1029-1037.
 
Yılmaz S, Ünal F, Yüzbaşıoğlu D (2009). The in vitro genotoxicity of benzoic acid in human peripheral blood lymphocytes. Cytotechnology 60(1-3):55-61.
Crossref
 
You Z, Brezzell M, Das S, Espadas-Torre M, Hooberman B, Sinsheimer J (1993). Ortho-Substituent effects on the in vitro and in vivo genotoxicity of benzidine derivatives. Mutat. Genet. 319(1):19-30.
Crossref
 
Yu S, Kawaguchi S, Kamaya A, Ohshita M, Kabasawa K, Iwama K, Tsuda S (2002). The comet assay with 8 mouse organs results with 39 currently used food additives. Mutat. Res. 519:103-119.
Crossref
 
Zeiger E, Anderson B, HaworthS, Lawlor T, Mortelmans K (1988). Salmonella mutagenicity tests: IV. Results from the testing of 300 chemicals. Environ. Mol. Mutagen. 11(S12):1-18.
Crossref
 
Zengin N, Yüzbaşıoğlu D, Ünal F, Yılmaz S, Aksoy H (2011). The evaluation of the genotoxicity of two food preservatives: Sodium benzoate and potassium benzoate. Food Chem. Toxicol. 49(4):763-769.
Crossref
 
Zimmermann T, Kulla H, Leisinger T (1982). Properties of Purified Orange II Azoreductase, the Enzyme Initiating Azo Dye Degradation by Pseudomonas KF46. Eur. J. Biochem. 129(1):197-203.
Crossref
 
Zuno A, Marcon F, Leopardi P, Salvatore G, Carere A, Crebelli R (1994). An assessment of the in vivo clastogenicity of erythrosine. Food Chem. Toxicol. 32(2):159-163.
Crossref

 


APA Olusegun, E.T., & Olajire, A.A. (2015). Toxicity of food colours and additives: A review. African Journal of Pharmacy and Pharmacology, 9(36), 900-914.
Chicago Olusegun E. Thomas and Olajire A. Adegoke. "Toxicity of food colours and additives: A review." African Journal of Pharmacy and Pharmacology 9, no. 36 (2015): 900-914.
MLA Olusegun E. Thomas and Olajire A. Adegoke. "Toxicity of food colours and additives: A review." African Journal of Pharmacy and Pharmacology 9.36 (2015): 900-914.
   
DOI 10.5897/AJPP2015.4385
URL http://www.academicjournals.org/journal/AJPP/article-abstract/4B78EBE55367

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