African Journal of Microbiology Research
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Article Number - B9B012766847


Vol.11(44), pp. 1600-1605 , November 2017
DOI: 10.5897/AJMR2017.8716
ISSN: 1996-0808



Full Length Research Paper

Extended-spectrum beta-lactamase- and carbapenemase-producing Enterobacteriaceae clinical isolates in a Senegalese teaching hospital: A cross sectional study



Makhtar Camara
  • Makhtar Camara
  • Laboratoire de Bactériologie-Virologie, Centre Hospitalier National Universitaire (CHNU) Aristide Le Dantec, Dakar, Sénégal.
  • Google Scholar
Mamadou Thierry Mane
  • Mamadou Thierry Mane
  • Laboratoire de Bactériologie-Virologie, Centre Hospitalier National Universitaire (CHNU) Aristide Le Dantec, Dakar, Sénégal.
  • Google Scholar
Awa Ba-Diallo
  • Awa Ba-Diallo
  • Laboratoire de Bactériologie-Virologie, Centre Hospitalier National Universitaire (CHNU) Aristide Le Dantec, Dakar, Sénégal.
  • Google Scholar
Assane Dieng
  • Assane Dieng
  • Laboratoire de Bactériologie-Virologie, Centre Hospitalier National Universitaire (CHNU) Aristide Le Dantec, Dakar, Sénégal.
  • Google Scholar
Halimatou Diop-Ndiaye
  • Halimatou Diop-Ndiaye
  • Laboratoire de Bactériologie-Virologie, Centre Hospitalier National Universitaire (CHNU) Aristide Le Dantec, Dakar, Sénégal.
  • Google Scholar
Farba Karam
  • Farba Karam
  • Laboratoire de Bactériologie-Virologie, Centre Hospitalier National Universitaire (CHNU) Aristide Le Dantec, Dakar, Sénégal.
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Seynabou Lo-Lo
  • Seynabou Lo-Lo
  • URF des Sciences de la Santé, Université Gaston Berger, Saint Louis, Sénégal.
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Habsa Diagne-Samb
  • Habsa Diagne-Samb
  • Laboratoire de Bactériologie-Virologie, Centre Hospitalier National Universitaire (CHNU) Aristide Le Dantec, Dakar, Sénégal.
  • Google Scholar
Safietou Ngom-Cisse
  • Safietou Ngom-Cisse
  • Laboratoire de Bactériologie-Virologie, Centre Hospitalier National Universitaire (CHNU) Aristide Le Dantec, Dakar, Sénégal.
  • Google Scholar
Coumba Toure-Kane
  • Coumba Toure-Kane
  • Institut de Recherche en Santé, de Surveillance Epidémiologique et de Formation (IRESSEF), Dakar, Sénégal.
  • Google Scholar
Aïssatou Gaye-Diallo
  • Aïssatou Gaye-Diallo
  • Laboratoire de Bactériologie-Virologie, Centre Hospitalier National Universitaire (CHNU) Aristide Le Dantec, Dakar, Sénégal.
  • Google Scholar
Souleymane Mboup
  • Souleymane Mboup
  • Laboratoire de Bactériologie-Virologie, Centre Hospitalier National Universitaire (CHNU) Aristide Le Dantec, Dakar, Sénégal.
  • Google Scholar
Cheikh Saad Bouh Boye
  • Cheikh Saad Bouh Boye
  • Laboratoire de Bactériologie-Virologie, Centre Hospitalier National Universitaire (CHNU) Aristide Le Dantec, Dakar, Sénégal.
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 Received: 23 September 2017  Accepted: 13 November 2017  Published: 28 November 2017

Copyright © 2017 Author(s) retain the copyright of this article.
This article is published under the terms of the Creative Commons Attribution License 4.0


Extended spectrum β-lactamase (ESBL) and carbapenemase-producing Enterobacteriaceae (CPE) have been increasingly reported worldwide. The objective of this study is to determine the prevalence of these multidrug-resistant strains in a major university teaching hospital in Dakar, Senegal. A total of 1205 Enterobacteriaceae stains were tested for ESBL and carbapenemase production. Antibiotics susceptibility test was performed with disk diffusion method. ESBL was detected using a double-disk synergy method. Carbapenemase production was detected with ertapenem 10 µg disk charge. The overall prevalence of ESBL- and carbapenemase-producing Enterobacteriaceae was 26.2 (316/1205) and 5.1% (62/1205), respectively. Interestingly, 3.8% of these pathogens were both ESBL-carbapenemase producers. Among the Enterobacteriaceae ESBL positive, Escherichia coli (45.2%, 143/316), Klebsiella pneumoniae (26.3%, 83/316), Enterobacter cloacae (12.7%, 40/316), and Proteus vulgaris (9.2%, 29/316) were the most prevalent. These strains were mainly isolated from urine (56.6%) and pus (22.7%) specimen. The most prevalent CPEs were E. coli (45.2%, 28/62), K. pneumoniae (27.4%, 17/62), and E. cloacae (16.1%, 10/62), particularly isolated from urine (58%) and pus (19.3%). The majority of these MDR strains were isolated from patients hospitalized in urology (32.4%), surgery (27.7%), internal medicine (18.5%), and intensive care units (10%). ESBL-producing Enterobacteriaceae remain highly susceptible to fosfomycin (94.1%), amikacin (92.5%) and ertapenem (88.6%), while carbapenemase producers were fully susceptible to amikacin (100%), and to a lesser extent, fosfomycin (66.7%) and colistin (60%). This study revealed increasing prevalence of ESBL- and carbapenemase-producing Enterobacteriaceae with limited therapeutic options, suggesting a need for continuous multi-drug resistant (MDR) surveillance patterns particularly in hospital settings.

Key words: Extended spectrum β-lactamase, carbapenemase, Enterobacteriaceae.

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APA Camara, M., Mane, M. T., Ba-Diallo, A., Dieng, A., Diop-Ndiaye, H., Karam, F., Lo-Lo, S., Diagne-Samb, H., Ngom-Cisse, S., Toure-Kane, C., Gaye-Diallo, A., Mboup, S., & Boye, C. S. B. (2017). Extended-spectrum beta-lactamase- and carbapenemase-producing Enterobacteriaceae clinical isolates in a Senegalese teaching hospital: A cross sectional study. African Journal of Microbiology Research, 11(44), 1600-1605.
Chicago Makhtar Camara, Mamadou Thierry Mane, Awa Ba-Diallo, Assane Dieng, Halimatou Diop-Ndiaye, Farba Karam, Seynabou Lo-Lo, Habsa Diagne-Samb, Safietou Ngom-Cisse, Coumba Toure-Kane, Aïssatou Gaye-Diallo, Souleymane Mboup, and Cheikh Saad Bouh Boye. "Extended-spectrum beta-lactamase- and carbapenemase-producing Enterobacteriaceae clinical isolates in a Senegalese teaching hospital: A cross sectional study." African Journal of Microbiology Research 11, no. 44 (2017): 1600-1605.
MLA Makhtar Camara, et al. "Extended-spectrum beta-lactamase- and carbapenemase-producing Enterobacteriaceae clinical isolates in a Senegalese teaching hospital: A cross sectional study." African Journal of Microbiology Research 11.44 (2017): 1600-1605.
   
DOI 10.5897/AJMR2017.8716
URL http://www.academicjournals.org/journal/AJMR/article-abstract/B9B012766847

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