African Journal of Microbiology Research
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Article Number - B665D5265077


Vol.11(25), pp. 1027-1034 , July 2017
DOI: 10.5897/AJMR2017.8586
ISSN: 1996-0808



Full Length Research Paper

Development of the fly ‘crop vessel’ bioassay for fly/microbial studies



Lufan Wang
  • Lufan Wang
  • Department of Food Sciences, College of Natural Sciences, University of Massachusetts, Amherst, MA. 01003, USA.
  • Google Scholar
John G. Stoffolano Jr.
  • John G. Stoffolano Jr.
  • College of Natural Sciences, Stockbridge School of Agriculture, University of Massachusetts, Amherst, MA. 01003, USA.
  • Google Scholar
Lynne Mclandsborough
  • Lynne Mclandsborough
  • Department of Food Sciences, College of Natural Sciences, University of Massachusetts, Amherst, MA. 01003, USA.
  • Google Scholar







 Received: 09 May 2017  Accepted: 29 June 2017  Published: 07 July 2017

Copyright © 2017 Author(s) retain the copyright of this article.
This article is published under the terms of the Creative Commons Attribution License 4.0


The dipteran crop has been implicated in many fly/microbe associations. In most cases, reports merely identify that the microbe(s) in question is(are) found inside the crop, but very few studies have focused on their long-term presence within the crop. Possibly, one of the main reasons for this is that flies usually regurgitate their crop contents, which makes it difficult to do long-term studies. Because of this, an in situ crop vessel bioassay was developed using adult Musca domestica as the crop donor and Escherichia coli are the microbe of choice. Procedures for crop filling, removal, sanitization and crop microbial contents over a 48 h incubation period were evaluated. Even though biofilm formation was suggested based on the microscopy, an increase in numbers was not observed, which possibly indicates there was no biofilm formed. This assay can be amended to fit any fly/microbe association in which one wants to study the microbe within the crop without its contents being regurgitated.

Key words: Musca domestica, diverticulated crop, biofilm, food safety, Escherichia coli.

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APA Wang, L., Stoffolano Jr., J. G., & Mclandsborough, L. (2017). Development of the fly ‘crop vessel’ bioassay for fly/microbial studies. African Journal of Microbiology Research, 11(25), 1027-1034.
Chicago Lufan Wang, John G. Stoffolano Jr. and Lynne Mclandsborough. "Development of the fly ‘crop vessel’ bioassay for fly/microbial studies." African Journal of Microbiology Research 11, no. 25 (2017): 1027-1034.
MLA Lufan Wang, et al. "Development of the fly ‘crop vessel’ bioassay for fly/microbial studies." African Journal of Microbiology Research 11.25 (2017): 1027-1034.
   
DOI 10.5897/AJMR2017.8586
URL http://www.academicjournals.org/journal/AJMR/article-abstract/B665D5265077

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