African Journal of
Microbiology Research

  • Abbreviation: Afr. J. Microbiol. Res.
  • Language: English
  • ISSN: 1996-0808
  • DOI: 10.5897/AJMR
  • Start Year: 2007
  • Published Articles: 4849

Full Length Research Paper

Assessment of microbiota in root canals with pulp necrosis by means of Gram test

Amaro de Mendonça Cavalcante
  • Amaro de Mendonça Cavalcante
  • Faculdade de Odontologia, Universidade Federal de Alagoas-57072-000-Maceió-AL, Brasil.
  • Google Scholar
Natalia Maria Luccas Tenório Soares
  • Natalia Maria Luccas Tenório Soares
  • Faculdade de Odontologia, Universidade Federal de Alagoas-57072-000-Maceió-AL, Brasil.
  • Google Scholar
Isabel Cristina Secandes Santos
  • Isabel Cristina Secandes Santos
  • Faculdade de Odontologia, Universidade Federal de Alagoas-57072-000-Maceió-AL, Brasil.
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Eulália Camelo Pessoa de Azevedo Ximenes
  • Eulália Camelo Pessoa de Azevedo Ximenes
  • Departamento de Antibióticos, Universidade Federal de Pernambuco-57040-521-Recife-PE, Brasil.
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Marcos Aurélio Bomfim da Silva
  • Marcos Aurélio Bomfim da Silva
  • Faculdade de Odontologia, Universidade Federal de Alagoas-57072-000-Maceió-AL, Brasil.
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Karlos Antonio Lisboa Ribeiro Junior
  • Karlos Antonio Lisboa Ribeiro Junior
  • Instituto de Química e Biotecnologia, Universidade Federal de Alagoas–57072-000-Maceió-AL, Brasil.
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  •  Received: 14 November 2017
  •  Accepted: 01 June 2018
  •  Published: 14 June 2018

Abstract

The aim of this study was to evaluate the type of microbiota present in root canals with pulp necrosis, with and without periapical lesion. Nineteen patients were selected for the study and 30 root canals were analysed in unirradicular and/or multi-radicular permanent teeth, asymptomatic, with pulp necrosis, with or without periapical lesion, and no communication between root canal and oral cavity. Absorbent paper cones were introduced in the canal for 20 s. Then, the contaminated paper cones were inoculated in a Brain Heart Infusion Agar-BHI culture medium and incubated in an oven for 48 h at 37°C. The data were analysed by non-parametric statistical method of the Cochran-test. The results revealed that there was no statistically significant difference between the amount of Gram-positive and negative bacteria in endodontic infections, indicating that the microbiota of these endodontic infections is mixed.

Key words: Microbiota, lipopolysaccharide, endodontics, root canals, pulp necrosis.